Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,144,381 G→C 9.4% V244L (GTG→CTG)  lpxK → tetraacyldisaccharide 4'‑kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,144,3810GC 9.4% 154.1 / 2.5 52V244L (GTG→CTG) lpxKtetraacyldisaccharide 4'‑kinase
Reads supporting (aligned to +/- strand):  ref base G (27/20);  new base C (2/3);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

CTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCATGACC  >  NC_002947/2144238‑2144524
                                                                                                                                               |                                                                                                                                               
cTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGcc                                                                                                                                            <  5:325626/149‑1 (MQ=255)
 tGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCg                                                                                                                                           >  1:266433/1‑149 (MQ=255)
     gcgGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCggcgg                                                                                                                                       <  1:135411/149‑4 (MQ=255)
       ggTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCg                                                                                                                                     <  5:75846/149‑1 (MQ=255)
           cTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCaa                                                                                                                                 >  7:336792/1‑149 (MQ=255)
                 aaCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGc                                                                                                                            >  5:152360/1‑148 (MQ=255)
                 aaCGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGc                                                                                                                            >  3:304528/1‑148 (MQ=255)
                            cGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCggcgggg                                                                                                                                     >  4:224212/1‑123 (MQ=39)
                            cGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCACCGCATGCAGGCGCTGGCCggcgggg                                                                                                                                     <  3:224212/128‑6 (MQ=39)
                             gAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCaa                                                                                                               >  4:82847/1‑149 (MQ=255)
                                        cGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTgg                                                                                                    <  8:336792/149‑1 (MQ=255)
                                         gATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTggg                                                                                                   <  3:243744/149‑1 (MQ=255)
                                                   gtttcTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTgg                                                                                         <  3:82847/149‑1 (MQ=255)
                                                              cAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGcc                                                                              >  5:109468/1‑149 (MQ=255)
                                                              cAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGc                                                                               <  4:304528/148‑1 (MQ=255)
                                                                     cGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATccc                                                                       <  6:109468/149‑1 (MQ=255)
                                                                        cccTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACAACCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCATc                                                                    >  7:86675/1‑149 (MQ=255)
                                                                          cTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGc                                                                  >  6:261544/1‑149 (MQ=255)
                                                                          cTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGc                                                                  >  8:132712/1‑149 (MQ=255)
                                                                              tCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTggg                                                                                                   <  8:79285/112‑1 (MQ=255)
                                                                              tCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTggg                                                                                                   >  7:79285/1‑112 (MQ=255)
                                                                                         aCTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGtt                                                   >  4:15628/1‑149 (MQ=255)
                                                                                           tGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGtt                                                   >  5:370501/1‑147 (MQ=255)
                                                                                           tGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGtt                                                   >  2:157313/1‑147 (MQ=255)
                                                                                                         cgcTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTg                                   <  7:297929/149‑1 (MQ=255)
                                                                                                         cgcTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTg                                   <  4:78859/149‑1 (MQ=255)
                                                                                                         ccatctaCCCTTTCCCCGCCGGCCAGCGCCTGCAGGCGGGGGCCGGTATCCGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGCCCATGCGCAGTTCAGTGCCCGGAGCCTg                                   <  4:230004/143‑1 (MQ=255)
                                                                                                          gcTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACg                                                                                                                        <  2:301466/63‑1 (MQ=255)
                                                                                                          gcTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACg                                                                                                                        >  1:301466/1‑63 (MQ=255)
                                                                                                            ttgACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGcc                                <  2:159427/147‑1 (MQ=255)
                                                                                                            tCGACCATTTCC‑‑‑CCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCtt                              >  7:167088/1‑148 (MQ=255)
                                                                                                              gACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCt                               >  4:108517/1‑148 (MQ=255)
                                                                                                                ccATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTg                                   >  3:230004/1‑142 (MQ=255)
                                                                                                                 cATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAg                           <  1:142755/149‑1 (MQ=255)
                                                                                                                      ccccGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGc                      >  8:181084/1‑149 (MQ=255)
                                                                                                                       gataccgGCCACCGCATGCAGGCGCTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGcc                     >  2:295767/5‑149 (MQ=255)
                                                                                                                       cccGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGc                      <  8:86675/148‑1 (MQ=255)
                                                                                                                           ccgGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTg                 <  6:370501/149‑1 (MQ=255)
                                                                                                                               ccAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGc             >  1:295503/1‑149 (MQ=255)
                                                                                                                                cAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCt            >  3:147070/1‑149 (MQ=255)
                                                                                                                                 accgcATGCAGGCGCTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTg           <  1:295767/147‑1 (MQ=255)
                                                                                                                                 aGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTg           >  1:312988/1‑149 (MQ=255)
                                                                                                                                  gcgcCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTgg          <  7:132712/149‑1 (MQ=255)
                                                                                                                                    gcCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTc        <  2:295503/149‑1 (MQ=255)
                                                                                                                                     ccTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTCCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCg              <  7:240592/142‑1 (MQ=255)
                                                                                                                                     ccTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTCCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCg              >  8:240592/1‑142 (MQ=255)
                                                                                                                                       tGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCAt      >  6:173406/1‑148 (MQ=255)
                                                                                                                                        gCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCATGa    >  8:93381/1‑149 (MQ=255)
                                                                                                                                         cATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGATCATGa    <  5:261544/148‑1 (MQ=255)
                                                                                                                                          aTGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCATGAcc  >  4:122776/1‑149 (MQ=255)
                                                                                                                                           tGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCATGAcc  >  5:149900/1‑148 (MQ=255)
                                                                                                                                            gCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACGATGCGCAGTTCGGTGCACGGCGCCTGGCCTTCAGCGGGGCACTGCCGAGGGTGATGGcc  >  8:244148/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                               
CTGACGCGGTGCTGTTCAACGGCGCCAGCGAAGACCGCACCGATGGTTTCGGTTTCTGCCTGCAGCCGTCGGCCCTGGTCAACGTGCGCACTGGCGAGCGCCGTGCGCTCGACCATTTCCCCGCCGGCCAGCGCCTGCATGCGGTGGCCGGTATCGGCAACCCGCAACGTTTCTTCAACACCCTGCTGGGGCTAAACTGGCAGCCGGTGCCGCATCCCTTCGCCGACCATGCGCAGTTCAGTGCCCGGAGCCTGGCCTTCAGCCCGCCACTGCCGCTGGTCATGACC  >  NC_002947/2144238‑2144524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑