Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,147,210 G→A 5.8% intergenic (+83/+34) murB → / ← rne UDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,147,2100GA 5.8% 265.1 / 3.4 87intergenic (+83/+34)murB/rneUDP‑N‑acetylenolpyruvoylglucosamine reductase/ribonuclease E
Reads supporting (aligned to +/- strand):  ref base G (48/33);  new base A (3/2);  total (51/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.51e-01

GGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACG  >  NC_002947/2147073‑2147357
                                                                                                                                         |                                                                                                                                                   
ggATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCgggg                                                                                                                                          <  7:108321/149‑1 (MQ=255)
  atatCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCt                                                                                                                                        <  3:254147/149‑1 (MQ=255)
    atCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgggg                                                                                                                                          <  1:179334/145‑1 (MQ=255)
    atCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgggg                                                                                                                                          >  2:179334/1‑145 (MQ=255)
       ttCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCttttt                                                                                                                                    >  5:346394/1‑148 (MQ=255)
       ttCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCttttt                                                                                                                                    >  2:13933/1‑148 (MQ=255)
         cGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCttttt                                                                                                                                    <  2:342314/146‑1 (MQ=255)
          gAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCa                                                                                                                                                   <  2:346509/130‑1 (MQ=255)
            gCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttcatt                                                                                                                                >  1:167713/1‑147 (MQ=255)
                  tGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCttt                                                                                                                         <  6:346394/148‑1 (MQ=255)
                  tGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTCCACTGGCGGGGCTTTTTCATTCAGCttt                                                                                                                         <  1:199546/148‑1 (MQ=255)
                    gggTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCtttt                                                                                                                                     >  5:189536/1‑134 (MQ=255)
                    gggTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCtttt                                                                                                                                     <  6:189536/134‑1 (MQ=255)
                    gggTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGc                                                                                                                      >  8:59389/1‑149 (MQ=255)
                      gTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCt                                                                                                                     >  2:200682/1‑148 (MQ=255)
                           gCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGc                                                                                                               >  8:331198/1‑149 (MQ=255)
                                     gAGCCTAATCTTTACTGATTTGCCGGCTGTGTACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAgg                                                                                                     >  8:275405/1‑149 (MQ=255)
                                     gAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttcatt                                                                                                                                >  2:213733/1‑122 (MQ=255)
                                     gAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttcatt                                                                                                                                <  1:213733/122‑1 (MQ=255)
                                     gAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAg                                                                                                      <  2:181331/148‑1 (MQ=255)
                                       gCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGtt                                                                                                   >  6:292154/1‑149 (MQ=255)
                                         cTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATc                                                                                                                  <  7:199409/132‑1 (MQ=255)
                                         cTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATc                                                                                                                  >  8:199409/1‑132 (MQ=255)
                                          tAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgt                                                                                                >  8:209655/1‑149 (MQ=255)
                                          tAATCTTTACTGATTTACCGGCGGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATAAGGTTtgt                                                                                                >  7:226392/1‑149 (MQ=255)
                                           aaTCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgtg                                                                                               >  3:27358/1‑149 (MQ=255)
                                              cTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCt                                                                                             >  4:112049/1‑148 (MQ=255)
                                                     gATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAAGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGtgatg                                                                                                        >  7:292005/1‑130 (MQ=255)
                                                     gATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAAGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGtgatg                                                                                                        <  8:292005/130‑1 (MQ=255)
                                                      aTTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATcggcg                                                                                     <  1:13933/148‑1 (MQ=255)
                                                      aTTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATcggcg                                                                                     <  2:167713/148‑1 (MQ=255)
                                                          gCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTc                                                                                >  3:147704/1‑149 (MQ=255)
                                                           ccGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttc                                                                                                                                   >  8:182828/1‑97 (MQ=255)
                                                           ccGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTttc                                                                                                                                   <  7:182828/97‑1 (MQ=255)
                                                           ccGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTc                                                                                >  6:32574/1‑148 (MQ=255)
                                                           ccGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTc                                                                                >  5:358227/1‑148 (MQ=255)
                                                              gCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgtg                                                                                               >  6:148160/1‑130 (MQ=255)
                                                              gCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTtgtg                                                                                               <  5:148160/130‑1 (MQ=255)
                                                                  tgAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCt                                                                         <  4:27358/148‑1 (MQ=255)
                                                                  tgAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTg                                                                        <  7:59389/149‑1 (MQ=255)
                                                                       ccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTcc                                                                   >  1:121063/1‑149 (MQ=255)
                                                                       ccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTcc                                                                   >  7:264233/1‑149 (MQ=255)
                                                                       ccccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTcc                                                                   >  4:7826/1‑149 (MQ=255)
                                                                        cccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCt                                                                  <  5:292154/149‑1 (MQ=255)
                                                                         ccGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTg                                                                 >  5:223613/1‑149 (MQ=255)
                                                                          cGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTgg                                                                <  7:209655/149‑1 (MQ=255)
                                                                            tAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTcc                                                                   <  1:252513/144‑1 (MQ=255)
                                                                            tAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTcc                                                                   >  2:252513/1‑144 (MQ=255)
                                                                            tAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGa                                                              <  6:358227/149‑1 (MQ=255)
                                                                               gAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTAATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCATGGCTGGTTCCTGGGAAGc                                                           >  3:86994/1‑149 (MQ=255)
                                                                                aGCGGGTTTACCCGCTAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTg                                                                        <  2:156183/135‑1 (MQ=255)
                                                                                aGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTg                                                                        >  1:156183/1‑135 (MQ=255)
                                                                                aGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCt                                                          >  3:296680/1‑149 (MQ=255)
                                                                                aGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACAAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTGTTTCATTCAGCTTTGGCTATCAGCAGTGATGAGGTTTGTGCTCATAGGCGGCTTCCAGGGCTGGTTCCTGGGAAg                                                            >  5:238524/1‑147 (MQ=255)
                                                                                  cGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgc                                                        <  3:112049/149‑1 (MQ=255)
                                                                                  cGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTg                                                         >  3:346230/1‑148 (MQ=255)
                                                                                     gTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcagc                                                     <  2:121063/149‑1 (MQ=255)
                                                                                           ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAg                                                                             <  6:242104/119‑1 (MQ=255)
                                                                                           ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAg                                                                             >  5:242104/1‑119 (MQ=255)
                                                                                           ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                               <  8:264233/149‑1 (MQ=255)
                                                                                           ccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                               <  7:331198/149‑1 (MQ=255)
                                                                                             gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                             >  7:26447/1‑149 (MQ=255)
                                                                                             gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgg                                                                                                                                            <  4:107371/54‑1 (MQ=18)
                                                                                             gcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTGCAAACTGGCgg                                                                                                                                            >  3:107371/1‑54 (MQ=18)
                                                                                              cgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtg                                            >  3:222080/1‑149 (MQ=255)
                                                                                               gAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtgt                                           <  7:275405/149‑1 (MQ=255)
                                                                                                aaGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtgtg                                          >  2:13361/1‑149 (MQ=255)
                                                                                                 agagGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTAGGAAGCTGCTGCAGCTTcccag                                           <  1:211185/147‑4 (MQ=255)
                                                                                                 agagGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTAGGAAGCTGCTGCAGCTTcccag                                           >  2:211185/1‑144 (MQ=255)
                                                                                                   agGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCg                                       >  2:33759/1‑149 (MQ=255)
                                                                                                       ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTggg                                                               <  2:313262/121‑1 (MQ=255)
                                                                                                       ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTggg                                                               >  1:313262/1‑121 (MQ=255)
                                                                                                       ccGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCttt                                   <  4:222080/149‑1 (MQ=255)
                                                                                                        cGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTg                                  >  5:350152/1‑149 (MQ=255)
                                                                                                            aGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgca                               <  6:223613/148‑1 (MQ=255)
                                                                                                              gCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcagca                            >  4:190473/1‑149 (MQ=255)
                                                                                                              gCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCATTTTCATACAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTACAGTGCCGCTTTGgcag                              >  2:214236/1‑147 (MQ=255)
                                                                                                                   ccAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTc                       >  5:369712/1‑149 (MQ=255)
                                                                                                                    cAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                      >  7:264618/1‑149 (MQ=255)
                                                                                                                    cAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                      >  5:154385/1‑149 (MQ=255)
                                                                                                                     aaaaaaaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCgg                     >  6:230915/1‑149 (MQ=255)
                                                                                                                       aaaaaGCCCCGCCAGTTTGCACTGGCGGGGCTTTATCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGcc                   >  5:80435/1‑149 (MQ=255)
                                                                                                                         aaaGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTc                 >  6:54314/1‑149 (MQ=255)
                                                                                                                            gCCCCGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCg              >  1:266898/1‑149 (MQ=255)
                                                                                                                              cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTc                       >  1:264856/1‑138 (MQ=255)
                                                                                                                              cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTc                       <  2:264856/138‑1 (MQ=255)
                                                                                                                                cGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTg          <  5:32574/149‑1 (MQ=255)
                                                                                                                                         gCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACg  >  6:223233/1‑148 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
GGATATCTTCGAGCGGTTTGGGGTTGAGCTGGAGATGGAGCCTAATCTTTACTGATTTGCCGGCTGTGAACCCCCGTAGGAGCGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGCCGTGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACG  >  NC_002947/2147073‑2147357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑