Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,149,451 C→G 10.0% E357Q (GAA→CAA)  rne ← ribonuclease E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,149,4510CG10.0% 176.1 / 9.3 60E357Q (GAA→CAA) rneribonuclease E
Reads supporting (aligned to +/- strand):  ref base C (25/28);  new base G (3/3);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

CCGGAGCAGCGTGGGCAGACGATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTT  >  NC_002947/2149303‑2149598
                                                                                                                                                    |                                                                                                                                                   
ccggAGCAGCGTGGGCAGACGATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTc                                                                                                                                                     <  2:308325/149‑1 (MQ=255)
  ggAGCAGCGTGGGCAGACGATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCaa                                                                                                                                                   <  1:287888/149‑1 (MQ=255)
             ggCAGACGATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTgg                                                                                                                                        <  3:118399/149‑1 (MQ=255)
                aGACGATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGttc                                                                                                                                     <  7:206642/149‑1 (MQ=255)
                aGACGATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGttc                                                                                                                                     <  7:155359/149‑1 (MQ=255)
                    gATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCg                                                                                                                                 >  3:288209/1‑149 (MQ=255)
                        ccGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTGTTGAAGAgcgcgtgcgtga                                                                                                                                     >  1:271206/1‑134 (MQ=255)
                        ccGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTGTTGAAGAgcgcgtgcgtga                                                                                                                                     <  2:271206/141‑8 (MQ=255)
                          gctgctTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCgggg                                                                                                                           <  4:288209/149‑1 (MQ=255)
                              tctgcGCCCATCGCTGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCAt                                                                                                                       >  8:196224/5‑149 (MQ=255)
                              ctTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTc                                                                                                                         <  3:198605/147‑1 (MQ=255)
                               tttCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATg                                                                                                                      >  4:206416/1‑149 (MQ=255)
                               tttCGCCCAGCGATGGGCGCAGACGCGGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATGCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATg                                                                                                                      >  2:203959/1‑149 (MQ=255)
                                 tCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGt                                                                                                                     <  3:206416/148‑1 (MQ=255)
                                 tCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGt                                                                                                                     <  4:16725/148‑1 (MQ=255)
                                   gCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGGGCAATAAATATCCAAACATTAACGCACGCGCTCTTAAACAGCGCGCTGGGTCTTAGCCCGGGTCATGTCGa                                                                                                                  >  1:115158/1‑149 (MQ=255)
                                   gCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGa                                                                                                                  >  2:200214/1‑149 (MQ=255)
                                       aGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGaa                                                                                                              <  4:162423/149‑1 (MQ=255)
                                            tGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGa                                                                                                         <  8:237479/149‑1 (MQ=255)
                                               gcgcAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGa                                                                                                      >  1:3125/1‑149 (MQ=255)
                                               gcgcAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTGCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAGGTCGATGa                                                                                                      >  8:226220/1‑149 (MQ=255)
                                                    gACGCTGACGGGACATATCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATc                                                                                                 <  2:36685/149‑1 (MQ=255)
                                                      cGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAg                                                                                               <  2:215629/149‑1 (MQ=255)
                                                                aCATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATg                                                                                     <  4:155914/149‑1 (MQ=255)
                                                                     tCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCAc                                                                                 <  1:200214/148‑1 (MQ=255)
                                                                                gAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCt                                                                     >  6:71108/1‑149 (MQ=255)
                                                                                gAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCt                                                                     >  4:4515/1‑149 (MQ=255)
                                                                                     gcgAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTggcgg                                                                <  7:196224/149‑1 (MQ=255)
                                                                                         gaTGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGa                                                            >  3:187948/1‑149 (MQ=255)
                                                                                           tGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGAtt                                                          <  5:71108/149‑1 (MQ=255)
                                                                                              ggCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGgccgcc                                                                                         >  8:157456/1‑115 (MQ=255)
                                                                                              ggCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGgccgcc                                                                                         <  7:157456/115‑1 (MQ=255)
                                                                                                ccAACCTGCACGCGGGCGCGGTCGGCTTCCAGTCATACACGCACGCGCTCTTCAACAGCGAGCTGGTTCTTCGAAGGCGTCATGAGGATGAAGTCGATGAATATCAGGACGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATATCCTc                                                     >  4:211391/1‑149 (MQ=255)
                                                                                                 cAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCg                                                    <  2:3125/149‑1 (MQ=255)
                                                                                                   aCCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGc                                                  >  1:58323/1‑149 (MQ=255)
                                                                                                      tGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCg                                                    <  3:235362/144‑1 (MQ=255)
                                                                                                      tGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCg                                                    >  4:235362/1‑144 (MQ=255)
                                                                                                       gCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCt                                              >  4:114695/1‑149 (MQ=255)
                                                                                                              ggCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGt                                       <  2:115158/149‑1 (MQ=255)
                                                                                                                cgcgGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTg                                     >  7:20791/1‑149 (MQ=255)
                                                                                                                cgcgGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTg                                     <  3:211391/149‑1 (MQ=255)
                                                                                                                    gTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCt                                 >  2:98112/1‑149 (MQ=255)
                                                                                                                     tCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTg                                <  4:187948/149‑1 (MQ=255)
                                                                                                                       ggCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTg                                >  2:10350/1‑147 (MQ=255)
                                                                                                                          ttCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAggg                           >  7:163113/1‑149 (MQ=255)
                                                                                                                              aGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGt                       >  7:161922/1‑149 (MQ=255)
                                                                                                                              aGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGt                       <  3:114695/149‑1 (MQ=255)
                                                                                                                                   ttCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTttctt                  <  8:163113/149‑1 (MQ=255)
                                                                                                                                       cgcacgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGAt              >  8:58116/1‑149 (MQ=255)
                                                                                                                                       ccagcgcgCTGTTGAAGAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTg                                     >  7:192564/5‑126 (MQ=38)
                                                                                                                                       ccagcgcgCTGTTGAAGAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTg                                     <  8:192564/122‑1 (MQ=38)
                                                                                                                                         agcgcgCTGTTGAAGAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGc                               >  4:261041/3‑130 (MQ=255)
                                                                                                                                         agcgcgCTGTTGAAGAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGc                               <  3:261041/128‑1 (MQ=255)
                                                                                                                                          acgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTggcgg                                                                <  1:86269/96‑1 (MQ=255)
                                                                                                                                          acgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTggcgg                                                                <  1:81426/96‑1 (MQ=255)
                                                                                                                                          acgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTggcgg                                                                >  2:81426/1‑96 (MQ=255)
                                                                                                                                          acgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTggcgg                                                                >  2:86269/1‑96 (MQ=255)
                                                                                                                                          acgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc           >  4:201880/1‑149 (MQ=255)
                                                                                                                                          acgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc           <  3:4515/149‑1 (MQ=255)
                                                                                                                                                   tCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTttcttc                 <  2:322336/134‑1 (MQ=255)
                                                                                                                                                   tCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTttcttc                 >  1:322336/1‑134 (MQ=255)
                                                                                                                                                    cAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGCCGATGAGTCCGCGGATGTCTCGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCTGGGCGGTTTCTTCGATGTCGCTGCCttt  <  1:203959/148‑1 (MQ=255)
                                                                                                                                                    cAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGc                               <  4:17463/119‑1 (MQ=255)
                                                                                                                                                    cAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGc                               >  3:17463/1‑119 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCGGAGCAGCGTGGGCAGACGATGCCGCTGCTTTCGCCCAGCGATGGGCGCAGACGCTGACGGGACATTTCCAGCAGGCCGAAGCGCGAGATGCGGCCAACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTT  >  NC_002947/2149303‑2149598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑