| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,165,573 | T→C | 6.9% | intergenic (‑36/+32) | PP_1920 ← / ← PP_5483 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,165,573 | 0 | T | C | 6.9% | 164.6 / 2.1 | 58 | intergenic (‑36/+32) | PP_1920/PP_5483 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (27/27); new base C (2/2); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
CGGATACGGATAACCCGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGCCTGATTTCTTAGCTCGCG > NC_002947/2165431‑2165721 | cggatacggataACCCGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGgtca < 8:323471/149‑1 (MQ=255) atacggataACCCGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGgtcagtc < 2:256482/149‑1 (MQ=255) cccGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGAc > 8:88296/1‑148 (MQ=255) ccGGTCTTTTTTGCGAAAATACCACCGTCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCt < 4:274714/142‑1 (MQ=255) ccGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCt > 3:274714/1‑142 (MQ=255) ccGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTgag > 6:168563/1‑145 (MQ=255) ccGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGGCATCTgag < 5:168563/145‑1 (MQ=255) ggTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCAACTGAGACtata < 6:318400/149‑1 (MQ=255) ctttttGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAg > 6:351767/2‑149 (MQ=255) aaaaTACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTgaga < 7:295029/131‑1 (MQ=255) aaaaTACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTgaga > 8:295029/1‑131 (MQ=255) tACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAg > 1:220828/1‑149 (MQ=255) gtagtTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGc < 3:301883/149‑1 (MQ=255) gtTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGTTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTg > 2:216511/1‑148 (MQ=255) ttttttGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCACCGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTgg > 5:339307/1‑148 (MQ=255) tcGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAg > 6:171733/1‑149 (MQ=255) tcGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAg > 1:117346/1‑149 (MQ=255) cGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATg > 3:203506/1‑142 (MQ=255) cGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATg < 4:203506/142‑1 (MQ=255) cGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTACCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGc > 2:168328/1‑149 (MQ=255) cGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTACCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGc > 2:162856/1‑149 (MQ=255) gTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGc < 7:88296/148‑1 (MQ=255) gTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCg > 2:211077/1‑149 (MQ=255) atTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGctct > 3:260978/1‑106 (MQ=255) atTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGctct < 4:260978/106‑1 (MQ=255) aTCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATTGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGAt > 1:112462/1‑143 (MQ=255) aTCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGAt < 2:112462/143‑1 (MQ=255) tGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGa < 7:71379/133‑1 (MQ=255) tGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGa > 8:71379/1‑133 (MQ=255) tGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCt > 8:145250/1‑148 (MQ=255) aGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTACCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATc < 1:168328/145‑1 (MQ=255) aGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTACCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATc < 1:162856/145‑1 (MQ=255) gCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAg < 2:220828/149‑1 (MQ=255) cGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCTGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGgtgt < 5:171733/149‑1 (MQ=255) ccATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGtaata > 8:27183/1‑147 (MQ=255) cATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTa < 8:77720/111‑1 (MQ=255) cATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTa > 7:77720/1‑111 (MQ=255) tAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTTGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAaagaa < 1:216511/149‑1 (MQ=255) tAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAaagaa < 5:351767/149‑1 (MQ=255) aTTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATgcgc > 5:56534/1‑125 (MQ=255) aTTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATgcgc < 6:56534/125‑1 (MQ=255) tttCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCt > 4:282561/1‑149 (MQ=255) cccTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTga < 1:211077/148‑1 (MQ=255) gCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGt < 7:27183/148‑1 (MQ=255) gCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGt > 5:274384/1‑148 (MQ=255) cTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTg < 2:117346/148‑1 (MQ=255) tGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCg < 7:75228/91‑1 (MQ=255) tGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCg > 8:75228/1‑91 (MQ=255) tAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCt > 3:306466/1‑149 (MQ=255) ggTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGc < 6:339307/149‑1 (MQ=255) ggTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGc < 3:261772/149‑1 (MQ=255) gtgtCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGt < 1:156065/138‑1 (MQ=255) gtgtCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGt > 2:156065/1‑138 (MQ=255) tttCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGCCTGATTTCt > 3:179755/1‑149 (MQ=255) tCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGCCTGATTTCt < 7:145250/147‑1 (MQ=255) gCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGgtgt > 2:92084/1‑108 (MQ=255) gCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGgtgt < 1:92084/108‑1 (MQ=255) cTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGCCTGATTTCt > 3:42571/1‑143 (MQ=255) tCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGCCTGATTTCTTAGCTcgcg < 4:306466/149‑1 (MQ=255) | CGGATACGGATAACCCGGTCTTTTTTGCGAAAATACCACCGGCCATAGTAGTTTTTTGTCTCGTAAATTGGGTATCCACATATTGTTATCTGAGCAACGCTTCCCATAGATTTCCCTTGCTGTAGGTTGTGTCTTTCCGCTATCGGTCAGTCATCTGAGACTATAGGAGCGATGTTAAATAGGCTCTGAAATTAGCTGGCATGCTCTAGCGGCTAGCCGAAGATAGCGATGCGCATCTGAAGGTGTAATAAAGAAGTCTGAGGAGTGAGCTGCCTGATTTCTTAGCTCGCG > NC_002947/2165431‑2165721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |