Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,231,466 T→C 8.9% L228P (CTG→CCG)  PP_1969 → molybdenum cofactor biosynthesis protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,231,4660TC 8.9% 121.0 / 3.0 45L228P (CTG→CCG) PP_1969molybdenum cofactor biosynthesis protein A
Reads supporting (aligned to +/- strand):  ref base T (20/21);  new base C (2/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTACTGCCCCTGCTGGATTACTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCTGCTCGCGGTTGCGCCT  >  NC_002947/2231328‑2231610
                                                                                                                                           |                                                                                                                                                
aGGTACTGCCCCTGCTGGATTACTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCggcggc                                                                                                                                        <  7:183732/149‑1 (MQ=255)
        ccccTGCTGGATTACTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGc                                                                                                                                >  4:151588/1‑149 (MQ=255)
        ccccTGCTGGATTACTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGc                                                                                                                                >  3:289996/1‑149 (MQ=255)
                 gATTACTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGccc                                                                                                                        <  1:356375/148‑1 (MQ=255)
                     aCTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGt                                                                                                                    <  1:96602/148‑1 (MQ=255)
                      cTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCAGCCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGt                                                                                                                    >  1:94229/1‑148 (MQ=255)
                       tGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGaa                                                                                                                 >  1:359561/1‑149 (MQ=255)
                         ccTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAAt                                                                                                                <  3:151588/148‑1 (MQ=255)
                         ccTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCCGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATg                                                                                                               >  5:137112/1‑149 (MQ=255)
                         ccTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCCGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATg                                                                                                               >  1:303346/1‑149 (MQ=255)
                         ccTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCCCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATg                                                                                                               >  3:293208/1‑149 (MQ=255)
                                 gcgGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTg                                                                                                       >  3:1731/1‑149 (MQ=255)
                                     cTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCC‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCacc                                                                                                                                    >  8:85351/1‑114 (MQ=255)
                                         gAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCAc                                                                                               <  6:157838/149‑1 (MQ=255)
                                         gAGCTGCGCTTCATCGAACTGATGCGCATCGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCa                                                                                                <  4:314009/148‑1 (MQ=255)
                                           gCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTg                                                                                             <  2:222888/149‑1 (MQ=255)
                                               cgcTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCa                                                                                          <  2:359561/148‑1 (MQ=255)
                                                     aTCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATACGTTa                                                                                   <  4:289996/149‑1 (MQ=255)
                                                         aaCTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACcac                                                                               <  4:1731/149‑1 (MQ=255)
                                                                   cATGGGCCA‑CCCCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCa                                                                     <  4:293208/149‑1 (MQ=255)
                                                                       ggCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAggg                                                                  <  2:94229/148‑1 (MQ=255)
                                                                         ccA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCCGCCTGCCTACGCCCAGGTGAATGCGCCGCTGGCCGCCCCTGCCATGCGTTACCACATCCCCGGCAGGGGc                                                                >  2:215186/1‑148 (MQ=255)
                                                                           a‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTAcc                                                                                 >  8:260087/1‑129 (MQ=255)
                                                                           a‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTAcc                                                                                 <  7:260087/129‑1 (MQ=255)
                                                                               gcgcccACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCCGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTc                                                          <  2:303346/144‑1 (MQ=255)
                                                                                 tctcACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCCGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTc                                                          <  6:137112/147‑1 (MQ=255)
                                                                                   tcACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAggg                                                                  <  7:301917/137‑1 (MQ=255)
                                                                                   tcACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAggg                                                                  >  8:301917/1‑137 (MQ=255)
                                                                                     aCGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGt                                                     >  2:132186/1‑148 (MQ=255)
                                                                                        accGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATgc                                                                                                              <  7:187727/86‑1 (MQ=255)
                                                                                        aTCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATgc                                                                                                              >  8:187727/1‑88 (MQ=255)
                                                                                           gCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGcc                                              <  1:132186/149‑1 (MQ=255)
                                                                                                   ttCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAg                                      >  5:337971/1‑149 (MQ=255)
                                                                                                           gcagTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGt                              >  1:114171/1‑149 (MQ=255)
                                                                                                                  gTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCg                       >  1:82679/1‑149 (MQ=255)
                                                                                                                    aGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTa                     >  1:177644/1‑149 (MQ=255)
                                                                                                                     ggCCGCGACCAGCTGCGTGCGCTGCTCGGCGGCCAGCATGCCTACGCCCCGGTGAAGGCGCCGCTGGACGCCACTGCCCTGCGTTACCACCTCCCCGGCCGGGGCCACTTCGGCGTCATTGCCAACGAACGCGTGCCGTTTTGCCGTAc                    <  1:215186/149‑1 (MQ=255)
                                                                                                                       ccTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCt                  >  6:256698/1‑149 (MQ=255)
                                                                                                                       ccTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCt                  >  3:339022/1‑149 (MQ=255)
                                                                                                                                      tGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCAtt                                                 >  1:147523/1‑103 (MQ=255)
                                                                                                                                      tGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCAtt                                                 <  2:147523/103‑1 (MQ=255)
                                                                                                                                       gcgcTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCTGCTCGCGGTTgcgc    <  6:337971/147‑1 (MQ=255)
                                                                                                                                       gcgcTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCTGCTCGCGGTTGCGCCt  >  3:5259/1‑149 (MQ=255)
                                                                                                                                        cgcTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCTGCTCGCGGTTGCGCCt  <  2:178699/148‑1 (MQ=255)
                                                                                                                                        cgcTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCTGCTCGCGGTTGCGCCt  <  2:177644/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
AGGTACTGCCCCTGCTGGATTACTGCCTCGCGCGCGGCTTCGAGCTGCGCTTCATCGAACTGATGCGCATGGGCCA‑CCTCTCTCACGATCGCAACGCCTTCCTCCAGCAGTTTGTAGGCCTCGACCAGCTGCTTGCGCTGATCGGCGGCCAGCATGCCTACGCCCAGGTGAATGCGCCGCTGGACGCCACTGCCATGCGTTACCACATCCCCGGCAGGGGCCACTTCGGCGTCATTGCCAACGAAAGCGTGCCGTTTTGCCGTACCTGCTCGCGGTTGCGCCT  >  NC_002947/2231328‑2231610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑