| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,241,568 | G→C | 6.8% | noncoding (35/76 nt) | PP_t25 → | tRNA‑Ala |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,241,568 | 0 | G | C | 6.8% | 187.7 / 3.8 | 59 | noncoding (35/76 nt) | PP_t25 | tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base G (28/27); new base C (2/2); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01 | |||||||||||
AAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCC > NC_002947/2241421‑2241714 | aaaaTTAGTTTGACAGCCCGGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatgg < 5:257183/148‑1 (MQ=255) gTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCag < 7:50445/148‑1 (MQ=255) tttGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCagga < 8:97016/149‑1 (MQ=255) tttGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGCCATGCaggc < 6:75445/149‑2 (MQ=255) gACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGt > 8:301128/1‑149 (MQ=255) gACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGt > 8:296231/1‑149 (MQ=255) cAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCa < 1:272390/149‑1 (MQ=255) cccTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCg > 7:60769/1‑149 (MQ=255) cccTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCg < 1:260357/149‑1 (MQ=255) cccTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGAATGCAGGAGGTCAGCg > 1:101668/1‑149 (MQ=255) ccTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCgg > 3:111722/1‑149 (MQ=255) gCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc > 2:190919/1‑149 (MQ=255) cGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATccc < 2:192053/149‑1 (MQ=255) ttAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCt > 1:178998/1‑147 (MQ=255) gcgcccGGCCACAGCGATCAACGAAAACGTACCGCCAGGCACCCAGCCAGCGATTCTGTTCAAAGCGACATAGTGGCTCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac < 4:35171/145‑1 (MQ=21) cgcCCCGTCCACAGCGATGTACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac < 1:143893/148‑1 (MQ=255) gcCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacc > 3:271242/1‑148 (MQ=255) gcCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacc > 3:297951/1‑148 (MQ=255) ccccGTCCACAGCGGTGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGt > 7:305077/1‑119 (MQ=255) ccccGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGt < 8:305077/119‑1 (MQ=255) cGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAAt > 5:201365/1‑148 (MQ=255) aGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac > 7:43595/1‑136 (MQ=255) aGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac < 8:43595/136‑1 (MQ=255) cGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCCCTTAGCTCCACCAAATTCCGCTTagc < 1:190919/149‑1 (MQ=255) aTGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTagcag > 2:233634/1‑149 (MQ=255) gAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTagcagc > 2:255896/1‑148 (MQ=255) acgaaaacgaaaCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTaa < 8:167231/149‑1 (MQ=255) cgaaaacgaaaCGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAg > 2:169739/1‑149 (MQ=255) cgaaacgCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAg < 7:83232/97‑1 (MQ=255) cgaaacgCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAg > 8:83232/1‑97 (MQ=255) gaaacgCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTag > 4:272572/1‑134 (MQ=255) gaaacgCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTag < 3:272572/134‑1 (MQ=255) gaaacgCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGAt > 8:1627/1‑148 (MQ=255) acgCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGc < 4:111722/149‑1 (MQ=255) gCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGc > 3:126024/1‑147 (MQ=255) ccAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGc > 2:93223/1‑146 (MQ=255) ggCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGAcc > 2:340767/1‑149 (MQ=255) cACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCgg < 4:271242/149‑1 (MQ=255) agccagcGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCa < 1:93223/149‑1 (MQ=255) agcGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCaa > 5:13367/1‑149 (MQ=255) aTTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCgg < 6:120429/149‑1 (MQ=255) cAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCg < 3:6119/102‑1 (MQ=255) cAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCg > 4:6119/1‑102 (MQ=255) aaaGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAg < 7:1627/149‑1 (MQ=255) aGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGt < 1:255896/149‑1 (MQ=255) aCATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTaag > 3:109048/1‑148 (MQ=255) tgGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTc < 8:60769/149‑1 (MQ=255) gggCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGAAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGt > 6:119578/1‑149 (MQ=255) tcccagctgagctgGGAGAGCGCCTGCATGGCATGCAGGATGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCaa < 5:25682/100‑1 (MQ=255) tcccagctgagctgGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCaa > 6:25682/5‑104 (MQ=255) tataGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTcccc > 2:14883/1‑149 (MQ=255) aGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTATTGAAGGTAAGAAGGTTCGTCCCCTTc < 1:14883/149‑1 (MQ=255) gCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTc < 4:297951/148‑1 (MQ=255) gCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAg < 4:141247/149‑1 (MQ=255) agagCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGcc > 7:180347/1‑148 (MQ=255) agCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAg < 6:201365/149‑1 (MQ=255) ctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTagc < 1:235176/63‑1 (MQ=255) ctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTagc > 2:235176/3‑65 (MQ=255) tccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGa > 7:41053/2‑148 (MQ=255) cTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGacac < 1:169739/149‑1 (MQ=255) tggcatgCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCGccc < 1:340767/149‑1 (MQ=255) | AAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATATGCGCCCCGTCCACAGCGATGAACGAAAACGAAACGCCAGGCACCCAGCCAGCGATTCAGTTCAAAGCGACATTGTGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAAATTCCGCTTAGCAGCTAAGGCTGATAAGCAAGACCGGGTCCAGCAACCGGGTCTTGAAGGTAAGAAGGTTCGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCC > NC_002947/2241421‑2241714 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |