Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,332	C→A	7.6%	R17R (CGG→CGT)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,332	0	C	A	7.6%	203.3 / 5.8	66	R17R (CGG→CGT)	trmE	GTPase
Reads supporting (aligned to +/- strand):  ref base C (36/25);  new base A (2/3);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

AGAGCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAG  >  NC_002947/6186‑6478                                                                                                                                                    |                                                                                                                                                   agagCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCg                                                                                                                                                  <  3:131457/149‑1 (MQ=255)   agCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCAGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGc                                                                                                                                                >  1:283139/1‑149 (MQ=255)     cAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGccc                                                                                                                                              >  1:150373/1‑149 (MQ=255)           cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGcc                                                                                                                                        >  8:3786/1‑149 (MQ=255)                      aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                             <  6:148016/149‑3 (MQ=255)                       ccAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtgg                                                                                                                            <  8:182581/149‑4 (MQ=255)                        cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                           >  4:301441/1‑149 (MQ=255)                        cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                           <  7:329004/149‑1 (MQ=255)                        cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGGCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGCGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                           >  8:58336/1‑149 (MQ=255)                             cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGAt                                                                                                                       <  1:89040/149‑1 (MQ=255)                                 aTCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGtt                                                                                                                   >  6:67205/1‑148 (MQ=255)                                 aTCGTCGCGGAATGGACCGTAATGGGAATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCTGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGtt                                                                                                                   >  8:155248/1‑148 (MQ=255)                                  tcgtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTc                                                                                                                 <  7:3786/149‑1 (MQ=255)                                                aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc                                                                                                    <  8:143173/148‑1 (MQ=255)                                                aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCa                                                                                                   >  4:352612/1‑149 (MQ=255)                                                    tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGAGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATgat                                                                                               >  6:360836/1‑147 (MQ=255)                                                        gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                             <  6:334034/115‑3 (MQ=38)                                                        gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                             >  5:334034/1‑113 (MQ=38)                                                         ggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                          <  3:352612/149‑1 (MQ=255)                                                            aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                       >  7:300213/1‑149 (MQ=255)                                                            aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                       >  8:86748/1‑149 (MQ=255)                                                               ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                              <  6:327852/139‑1 (MQ=255)                                                               ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                              >  5:327852/1‑139 (MQ=255)                                                                cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGGCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGGCGTGGCGATTGCGGCGATGGTTTCACGGACAGTGGTCATGCTCGAAAGCCTCTa                                                                                   <  5:360836/149‑1 (MQ=255)                                                                 gcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                  >  4:334401/1‑149 (MQ=255)                                                                       tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaa                                                                             >  5:345328/1‑148 (MQ=255)                                                                           ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                         >  8:34920/1‑148 (MQ=255)                                                                             tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                      <  7:86748/149‑1 (MQ=255)                                                                                 gCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                  >  7:311462/1‑149 (MQ=255)                                                                                 gCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                  <  7:155248/149‑1 (MQ=255)                                                                                   cGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                   >  3:131079/1‑146 (MQ=255)                                                                                      tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCa                                                              >  5:205462/1‑148 (MQ=255)                                                                                           aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                         <  3:334401/148‑1 (MQ=255)                                                                                            aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                       >  2:334053/1‑149 (MQ=255)                                                                                                gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                   >  6:224936/1‑149 (MQ=255)                                                                                                gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                   >  2:131220/1‑149 (MQ=255)                                                                                                gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                   >  5:81461/1‑149 (MQ=255)                                                                                                gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCAAGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                   >  8:277016/1‑149 (MQ=255)                                                                                                     ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                          >  7:64888/1‑105 (MQ=255)                                                                                                     ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                          <  8:64888/105‑1 (MQ=255)                                                                                                     ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAgg                                              <  5:67205/149‑1 (MQ=255)                                                                                                         ggCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAgggg                                            <  6:345328/147‑1 (MQ=255)                                                                                                           cAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                >  2:62537/1‑109 (MQ=255)                                                                                                           cAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                <  1:62537/109‑1 (MQ=255)                                                                                                             gCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGttt                                      <  4:310833/149‑1 (MQ=255)                                                                                                                    ggCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTc                               <  6:205462/149‑1 (MQ=255)                                                                                                                               ccTGACAATTCCCACACCGCCCCGGCCCTGGGCAGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTAATGCTCGAAAGCCTCTACGACAAAAACGACAGAAAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTAACAGGATAGc                     >  2:71735/1‑149 (MQ=255)                                                                                                                                cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                     >  3:227367/1‑68 (MQ=255)                                                                                                                                cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                     <  4:227367/68‑1 (MQ=255)                                                                                                                                cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCg                    >  1:45881/1‑149 (MQ=255)                                                                                                                                  gACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGAGGTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACAAGGGGGCGTTTATTATTCACAGGCTAGCGTa                  >  4:237769/1‑149 (MQ=255)                                                                                                                                   aCAATTCCCACACCGCCCCGGCCCTGGGCGGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTa                  <  2:283139/148‑1 (MQ=255)                                                                                                                                    cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAtt                <  5:224936/149‑1 (MQ=255)                                                                                                                                    cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAtt                <  2:76934/149‑1 (MQ=255)                                                                                                                                        tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTcaca                            >  2:292739/1‑133 (MQ=255)                                                                                                                                        tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGCCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTcaca                            <  1:292739/133‑1 (MQ=255)                                                                                                                                          ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg          >  6:107083/1‑149 (MQ=255)                                                                                                                                          ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg          >  6:258032/1‑149 (MQ=255)                                                                                                                                           cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                     <  1:57929/121‑1 (MQ=255)                                                                                                                                           cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                     >  2:57929/1‑121 (MQ=255)                                                                                                                                           cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCCCAGGCTAGCGTATTAGCCCGt         >  8:67483/1‑149 (MQ=255)                                                                                                                                            ccatcGCCACGGCCCAGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt         >  7:90100/5‑148 (MQ=255)                                                                                                                                            acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAACAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc        <  3:301441/149‑1 (MQ=255)                                                                                                                                            acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCCCTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt         >  3:222117/1‑148 (MQ=255)                                                                                                                                              acCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCaa      <  2:150373/149‑1 (MQ=255)                                                                                                                                                cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag     >  4:59370/1‑148 (MQ=255)                                                                                                                                                  ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag  >  5:234092/1‑149 (MQ=255)                                                                                                                                                  ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag  >  2:52805/1‑149 (MQ=255)                                                                                                                                                   cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag  >  2:186182/1‑148 (MQ=255)                                                                                                                                                   cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag  <  1:186182/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AGAGCAATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAG  >  NC_002947/6186‑6478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑