Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,273,880 C→A 8.4% G163C (GGC→TGC)  PP_2004 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,273,8800CA 8.4% 412.5 / 21.3 131G163C (GGC→TGC) PP_2004AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (57/62);  new base A (6/5);  total (63/68)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

Only 100 of 136 total aligned reads displayed.

TGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGC  >  NC_002947/2273733‑2274027
                                                                                                                                                   |                                                                                                                                                   
tGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCg                                                                                                                                                    >  2:34652/1‑149 (MQ=255)
  gCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCgg                                                                                                                                                   <  2:276032/148‑1 (MQ=255)
  gCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGc                                                                                                                                                  <  4:192941/149‑1 (MQ=255)
  gCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCGGCACCCCGGTGCAGGc                                                                                                                                                  <  5:298762/149‑1 (MQ=255)
   ccAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCGGCACCCCGGTGCAGGcc                                                                                                                                                 <  5:177714/149‑1 (MQ=255)
      gTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGc                                                                                                                                              <  6:140746/149‑1 (MQ=255)
      gTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGc                                                                                                                                              >  6:321415/1‑149 (MQ=255)
       ttGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCt                                                                                                                                             >  7:313514/1‑149 (MQ=255)
          cAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCgg                                                                                                                                          >  2:69395/1‑149 (MQ=255)
          cAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCg                                                                                                                                           <  4:176911/148‑1 (MQ=255)
             ggTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGcc                                                                                                                                        >  8:199566/1‑148 (MQ=255)
               tAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAAc                                                                                                                                     <  3:352193/149‑1 (MQ=255)
                aGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACt                                                                                                                                    >  5:130057/1‑149 (MQ=255)
                  cgcgGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTgg                                                                                                                                  <  3:205758/149‑1 (MQ=255)
                  cgcgGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACAGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTgg                                                                                                                                  >  1:255656/1‑149 (MQ=255)
                   gcgGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTggg                                                                                                                                 <  7:191393/149‑1 (MQ=255)
                    cgGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGc                                                                                                                                              <  3:7467/135‑1 (MQ=255)
                    cgGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGc                                                                                                                                              >  4:7467/1‑135 (MQ=255)
                     ggCTCAGACGCAGTTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgcc                                                                                                                               <  1:266411/149‑1 (MQ=255)
                      gCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgcca                                                                                                                              <  5:22987/149‑1 (MQ=255)
                       cTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgccag                                                                                                                             >  3:77700/1‑149 (MQ=255)
                        tCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCGCAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgccagc                                                                                                                            >  3:65489/1‑149 (MQ=255)
                          aGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCTTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgccagc                                                                                                                            <  6:324983/147‑1 (MQ=255)
                           gACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGccagccag                                                                                                                         >  2:149023/1‑149 (MQ=255)
                             cGCATCTGATTGCCTTCGGAGGCGAACTGCACAGGATTGCTGCAAAGCGGTTGATAGAGCTCGGCATAGTAGTGCGCGGGAATCTCTATGTCCAGGCGTTCTTCCTGAACAGGGGTGCCTTCCAGCTGGGCGAACTGGGCGagacatcc                                                                                                                       <  8:4187/149‑1 (MQ=255)
                              gCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCg                                                                                                                      >  3:130767/1‑149 (MQ=255)
                                  cTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCca                                                                                                                  <  4:135060/149‑1 (MQ=255)
                                    gATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCcag                                                                                                                 >  4:56253/1‑148 (MQ=255)
                                       tGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgccagc                                                                                                                            <  2:4172/134‑1 (MQ=255)
                                        gCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGGTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGATCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGt                                                                                                             <  4:214378/148‑1 (MQ=255)
                                         ccGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGt                                                                                                             >  5:152713/1‑147 (MQ=255)
                                         ccGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTg                                                                                                            <  7:199566/148‑1 (MQ=255)
                                              ggcggcGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGa                                                                                                      <  7:15377/149‑1 (MQ=255)
                                               gcggcgAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGAc                                                                                                     <  1:9683/149‑1 (MQ=255)
                                                cggcgAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGAc                                                                                                     >  6:130370/1‑148 (MQ=255)
                                                 ggcgAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACca                                                                                                   >  5:108161/1‑149 (MQ=255)
                                                       cTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGa                                                                                                      <  4:107517/140‑1 (MQ=255)
                                                        tGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCg                                                                                            >  2:274630/1‑149 (MQ=255)
                                                        tGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCg                                                                                            <  6:152713/149‑1 (MQ=255)
                                                          cacaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgc                                                                                                                                >  7:97979/1‑111 (MQ=255)
                                                          cacaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGgc                                                                                                                                <  8:97979/111‑1 (MQ=255)
                                                           acaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                         <  1:69395/149‑1 (MQ=255)
                                                           acaGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                         <  8:313514/149‑1 (MQ=255)
                                                             aGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGt                                                                                                             >  1:104469/1‑127 (MQ=255)
                                                             aGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGt                                                                                                             <  2:104469/127‑1 (MQ=255)
                                                                   gctgcAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGtt                                                                                         >  5:137826/1‑141 (MQ=255)
                                                                   gctgcAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGt                                                                                 >  8:229869/1‑149 (MQ=255)
                                                                   gctgcAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGt                                                                                 <  4:82311/149‑1 (MQ=255)
                                                                    ctgcAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGtt                                                                                <  6:130057/149‑1 (MQ=255)
                                                                     tgcAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCGGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCg                                                                                            <  1:55288/136‑1 (MQ=255)
                                                                     tgcAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCcagccagc                                                                                                                        <  6:203860/108‑1 (MQ=255)
                                                                       cAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTg                                                                               >  7:9947/1‑147 (MQ=255)
                                                                        aaaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTg                                                                                        >  5:59276/1‑137 (MQ=255)
                                                                        aaaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTg                                                                                        <  6:59276/137‑1 (MQ=255)
                                                                        aaaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAg                                                                            >  6:100914/1‑149 (MQ=255)
                                                                         aaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTa                                                                             >  4:244957/1‑147 (MQ=255)
                                                                         aaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTa                                                                             <  3:244957/147‑1 (MQ=255)
                                                                         aaGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAgg                                                                           >  4:94660/1‑149 (MQ=255)
                                                                           gCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAgggg                                                                         <  1:149023/149‑1 (MQ=255)
                                                                                  aTAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATg                                                                   <  5:65799/148‑1 (MQ=255)
                                                                                     gcgcgcGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGaa                                                                <  1:34652/148‑1 (MQ=255)
                                                                                      cgcgcgGGATAGGAGGGCGCGGCAAAGTCGATCTCCAGGCGTTCGGCCTGCACGGGGGTGCCGGCCAGCTGGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTTGTCGTTGTAGTGGCTGATGGAATa                                                              <  1:54361/149‑1 (MQ=255)
                                                                                       gcgcgGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGGTGTAGTCGTTGTAGGGGCTGATGGaa                                                                >  4:8632/1‑146 (MQ=255)
                                                                                                aggaggGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAg                                                                                              >  7:4219/1‑107 (MQ=255)
                                                                                                aggaggGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCg                                                                                            <  6:121703/109‑1 (MQ=255)
                                                                                                  gaggGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGc                                                    >  4:53628/1‑147 (MQ=255)
                                                                                                            cAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGCCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGAAGTCGTAGTAGGGGCTGAAGGAATAGAAgc                                                         >  2:24576/1‑132 (MQ=255)
                                                                                                             aaaCTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTggg                                       >  7:271038/1‑149 (MQ=255)
                                                                                                               aCTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGAc                                                                                                     >  6:274523/1‑85 (MQ=255)
                                                                                                               aCTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGAc                                                                                                     <  5:274523/85‑1 (MQ=255)
                                                                                                                 tCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTggg                                       >  4:163279/1‑145 (MQ=255)
                                                                                                                 tCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTggg                                       <  3:163279/145‑1 (MQ=255)
                                                                                                                   gATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATc                                   <  3:94660/147‑1 (MQ=255)
                                                                                                                    aTCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGa                                                                                                           <  3:88523/74‑1 (MQ=255)
                                                                                                                      ctcCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTcc                               <  4:130767/148‑1 (MQ=255)
                                                                                                                          aGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCa                            >  3:297782/1‑147 (MQ=255)
                                                                                                                              gTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCgg                      <  8:231457/149‑1 (MQ=255)
                                                                                                                                 cGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGa                    <  7:229869/148‑1 (MQ=255)
                                                                                                                                  ggCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGa                    <  1:279020/147‑1 (MQ=255)
                                                                                                                                  ggCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAAt                  >  5:256025/1‑149 (MQ=255)
                                                                                                                                  ggCCGGCACCCCGGTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTggg                                       >  8:283328/1‑128 (MQ=255)
                                                                                                                                  ggCCGGCACCCCGGTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTggg                                       <  7:283328/128‑1 (MQ=255)
                                                                                                                                  ggCCGGCACCCCGGTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAAt                  >  6:16477/1‑149 (MQ=255)
                                                                                                                                   gCCGGCACCCCGGTGCAGGCCAGCTCGGCCTACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATg                 >  1:31912/1‑149 (MQ=255)
                                                                                                                                   gCCGGCACCCCGGTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTc                                >  8:87176/1‑134 (MQ=255)
                                                                                                                                     cTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCg                       >  6:233407/1‑141 (MQ=255)
                                                                                                                                     cTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCg                       <  5:233407/141‑1 (MQ=255)
                                                                                                                                     cTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGc               >  2:64860/1‑149 (MQ=255)
                                                                                                                                      tGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGcc              >  3:188873/1‑149 (MQ=255)
                                                                                                                                      tGCACCGGGGTGCCGACCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGcc              >  3:305419/1‑149 (MQ=255)
                                                                                                                                         aCCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTgg                                        >  3:248945/1‑120 (MQ=255)
                                                                                                                                         aCCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTgg                                        <  4:248945/120‑1 (MQ=255)
                                                                                                                                           cGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCt                              >  8:168664/1‑128 (MQ=255)
                                                                                                                                           cGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCt                              <  7:168664/128‑1 (MQ=255)
                                                                                                                                               gTGCAGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGtt     <  2:31912/149‑1 (MQ=255)
                                                                                                                                                  ccGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGc  >  7:279087/1‑149 (MQ=255)
                                                                                                                                                   cGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcgc                                                       <  1:262877/95‑1 (MQ=255)
                                                                                                                                                   cGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAgcgc                                                       >  2:262877/1‑95 (MQ=255)
                                                                                                                                                   cGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCg                       >  1:238107/1‑127 (MQ=255)
                                                                                                                                                   cGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCg                       <  2:238107/127‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGCCAGTTGCAAGGTAGCGCGGCTCAGACGCAGCTGATTGCCGTCGGCGGCGAACTGCACAGGCGTGCTGCAAAGCGGTTGATAGCGCGCGGCATAGGAGGGCGCGGCAAACTCGATCTCCAGGCGTTCGGCCTGCACCGGGGTGCCGGCCAGCTCGGCCAACTGGGCCAGCCAGCCGGCCAGCAGTGAGTCGACCACAAAGCGGTTGTAGTCGTTGTAGGGGCTGATGGAATAGAAGCGCAGCCAGGCGCCCTGGGCATCTTCCTCAAAGCGGGAATGGCCGCGGTAGTTGGC  >  NC_002947/2273733‑2274027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑