Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,294,021	T→A	6.0%	L6M (TTG→ATG)	PP_2020 →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,294,021	0	T	A	6.0%	254.3 / 5.0	83	L6M (TTG→ATG)	PP_2020	lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (36/42);  new base A (3/2);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

GGCCTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACT  >  NC_002947/2293874‑2294164                                                                                                                                                    |                                                                                                                                                ggCCTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCAtt                                                                                                                                                <  2:269366/149‑1 (MQ=255)   ccTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGc                                                                                                                                              <  1:87648/149‑1 (MQ=255)    cTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCg                                                                                                                                             <  5:297157/149‑1 (MQ=255)       aTGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCgg                                                                                                                                            <  1:4619/147‑1 (MQ=255)        tGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGccc                                                                                                                                         >  5:294194/1‑149 (MQ=255)           tGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGcccccc                                                                                                                                      >  8:229194/1‑149 (MQ=255)           tGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGcccccc                                                                                                                                      >  4:171366/1‑149 (MQ=255)           tGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCAATGCGGccccct                                                                                                                                      <  2:352226/149‑2 (MQ=255)             tccTTCATGCTGCTTTGTAACTTGTTTGGTGCGCTGTGGCTGCTACGGGGATTGGCGCGGTTCCTGATCACGCCTGAGAAGTTGGTGGGCATGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCgg                                                                                                                                    <  4:47627/148‑1 (MQ=255)             aCCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGcccccc                                                                                                                                      <  4:347378/147‑1 (MQ=255)             aCCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCgg                                                                                                                                    >  8:37971/1‑149 (MQ=255)             aCCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCAATGCGGccccct                                                                                                                                      <  2:243458/147‑2 (MQ=255)             aCCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGGCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCgg                                                                                                                                    <  5:337898/149‑1 (MQ=255)                  cATGCTGCTTTGTAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTta                                                                                                                                 <  7:197847/147‑1 (MQ=255)                      ctgctTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGc                                                                                                                           <  7:229194/149‑1 (MQ=255)                          tttGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGc                                                                                                                       >  7:24469/1‑149 (MQ=255)                          tttGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGc                                                                                                                       >  5:78992/1‑149 (MQ=255)                              gAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCa                                                                                                                    <  2:281993/148‑1 (MQ=255)                               aaCATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCAtt                                                                                                                  >  5:31916/1‑149 (MQ=255)                                aaaTGTTTGGTGCGCTGTGGCTGGTACCGGAATTTGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCAtt                                                                                                                  <  4:65524/146‑1 (MQ=255)                                 cATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTc                                                                                                                <  6:31916/149‑1 (MQ=255)                                  aTGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCa                                                                                                               <  8:3411/149‑1 (MQ=255)                                  aTGTTTGGTCCTCTGTGGCTGCTACCGGCATTGTTGCGGTGACTGATCCAGCCTGAGAAGATGGTGGGAAAGGTGTGTGGGTCCAGATTCGCCCATGGATTGGAGTGTGCCGCATTGGGTCCCCCCGGTTATACTTCCAGCTCATTTCa                                                                                                               <  4:38358/149‑1 (MQ=255)                                    gTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCa                                                                                                                         >  5:328925/1‑137 (MQ=255)                                    gTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCa                                                                                                                         <  6:328925/137‑1 (MQ=255)                                    gTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCAttt                                                                                                                 >  8:43374/1‑145 (MQ=255)                                      ttGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGAt                                                                                                            <  5:36424/148‑1 (MQ=255)                                       tGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATg                                                                                                           <  8:24469/148‑1 (MQ=255)                                        ggTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATgg                                                                                                          <  2:253463/148‑1 (MQ=255)                                        ggTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATgg                                                                                                          <  7:18262/148‑1 (MQ=255)                                        ggTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATgg                                                                                                          <  7:24936/148‑1 (MQ=255)                                         gTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGt                                                                                                        <  6:294194/149‑1 (MQ=255)                                                   gctgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCg                                                                                                                                             <  6:273963/102‑1 (MQ=255)                                                     tgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTcc                                                                                            >  1:68337/1‑149 (MQ=255)                                                     tgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTcc                                                                                            >  8:334288/1‑149 (MQ=255)                                                     tgctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTcc                                                                                            >  8:83666/1‑149 (MQ=255)                                                      gctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCGAGCTCATTTCAGATgg                                                                                                          >  2:252666/1‑134 (MQ=255)                                                      gctACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGCCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATgg                                                                                                          <  1:252666/134‑1 (MQ=255)                                                           cGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTc                                                                                      >  1:22567/1‑149 (MQ=255)                                                           cGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTc                                                                                      <  6:78992/149‑1 (MQ=255)                                                           cGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTc                                                                                      >  3:196258/1‑149 (MQ=255)                                                            ggCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTta                                                                                                                                 >  1:110006/1‑105 (MQ=255)                                                            ggCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTta                                                                                                                                 <  2:110006/105‑1 (MQ=255)                                                             gCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGt                                                                                                        >  2:36073/1‑129 (MQ=255)                                                             gCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGt                                                                                                        <  1:36073/129‑1 (MQ=255)                                                                ttGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTAc                                                                                 <  7:43374/149‑1 (MQ=255)                                                                ttGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTAc                                                                                 <  8:37215/149‑1 (MQ=255)                                                                       ggTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTg                                                                          <  7:37971/149‑1 (MQ=255)                                                                             tGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTcc                                                                    >  8:92034/1‑149 (MQ=255)                                                                              gATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCATGCAGTCCt                                                                   >  4:229221/1‑149 (MQ=255)                                                                               aTCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCTTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTg                                                                  >  5:77096/1‑149 (MQ=255)                                                                                tCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCAttt                                                                                                                 >  3:101185/1‑101 (MQ=255)                                                                                tCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCAttt                                                                                                                 <  4:101185/101‑1 (MQ=255)                                                                                     ccTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTTCCGCCCTGCAGTCCTGAGctat                                                            >  7:89980/1‑149 (MQ=255)                                                                                        gagaAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTg                                                                  <  1:334978/140‑1 (MQ=255)                                                                                            agaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCtttt                                                      >  3:299815/1‑148 (MQ=255)                                                                                             gaTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGa                                                    <  3:171366/149‑1 (MQ=255)                                                                                              aTGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGAt                                                   <  2:68337/149‑1 (MQ=255)                                                                                                  tggGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGAc                                               <  4:196258/149‑1 (MQ=255)                                                                                                     gCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGc                                            >  4:76408/1‑149 (MQ=255)                                                                                                          gaggGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTg                                                                  <  2:5192/122‑1 (MQ=255)                                                                                                          gaggGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTg                                                                  >  1:5192/1‑122 (MQ=255)                                                                                                                   tCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCACGGTTATACTGCCAGCGCATTTGAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGa                              >  1:344547/1‑149 (MQ=255)                                                                                                                    cGATCTTCCCCCATTGATGGGAGGGGGCCGCATGGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGAt                             <  7:285861/149‑1 (MQ=255)                                                                                                                     gATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGAtt                            >  3:93536/1‑149 (MQ=255)                                                                                                                        cTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCa                         >  3:260360/1‑149 (MQ=255)                                                                                                                         ttCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACACCTATGTCCCCCCTCGCTACCGCCCTGCAGACCTCCGATATGCTTTTGATCGACCGCCTGAACGCCTTCGAATTCa                         >  7:189363/1‑148 (MQ=255)                                                                                                                           cGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCa                         <  3:172875/146‑1 (MQ=255)                                                                                                                            gCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGt                     <  7:334288/149‑1 (MQ=255)                                                                                                                             cccATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGtt                    <  7:83666/149‑1 (MQ=255)                                                                                                                              ccATTGATTGGTGGGGGCCGCATTGCGGCCCCCCGGTTATACTGc                                                                                                                           <  8:197247/45‑1 (MQ=255)                                                                                                                              ccATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTtg                   <  4:177591/149‑1 (MQ=255)                                                                                                                              ccATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTtg                   <  4:299815/149‑1 (MQ=255)                                                                                                                                attgattgGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTtgat                 >  2:89163/1‑149 (MQ=255)                                                                                                                                       ccggggggCCGCAATGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCg          >  4:58769/4‑149 (MQ=255)                                                                                                                                        gAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCGGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCgg         >  1:189537/1‑149 (MQ=255)                                                                                                                                        cggggggCCGCAATGCGGCCCCCTGGTTATACTGCCAGCTCATTTGAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCgg         >  4:254710/3‑149 (MQ=255)                                                                                                                                        cggggggCCGCAATGCGGCCCCCTGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATAGGCTTTTGATCGACGGCCTGCCCGCCGTCGATTTCCCCGTTGAGGAAGCCgg         >  4:259386/3‑149 (MQ=255)                                                                                                                                         aGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGc        >  1:5989/1‑149 (MQ=255)                                                                                                                                         aGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGc        >  8:91963/1‑149 (MQ=255)                                                                                                                                             ggCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCT‑CACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCAc   >  3:114711/1‑149 (MQ=255)                                                                                                                                               ccGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCCATATGCTTTTGATCGACGGCCTGCACGCCGTCGATTTCGCCGTTGATGACTCCGGCCTCACt  >  4:4991/1‑149 (MQ=255)                                                                                                                                                cGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCa                         <  3:225108/125‑1 (MQ=255)                                                                                                                                                cGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCa                         >  4:225108/1‑125 (MQ=255)                                                                                                                                                    ttGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCa                                                                        <  2:266275/74‑1 (MQ=255)                                                                                                                                                    ttGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCa                                                                        >  1:266275/1‑74 (MQ=255)                                                                                                                                                    |                                                                                                                                                GGCCTGATGCTGACCTTCATGCTGCTTTGGAACATGTTTGGTGCGCTGTGGCTGCTACCGGCATTGGCGCGGTTCCTGATCAAGCCTGAGAAGATGGTGGGCAAGGAGGGTGGGTCGATCTTCGCCCATTGATTGGAGGGGGCCGCATTGCGGCCCCCCGGTTATACTGCCAGCTCATTTCAGATGGGTACCCCTATGTCCCCCCTCGCTACCGCCCTGCAGTCCTGCGATATGCTTTTGATCGACGGCCTGCACGCCTTCGATTTCACCGTTGATGAAGCCGGCCTCACT  >  NC_002947/2293874‑2294164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑