BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,308,753	T→C	5.7%	Q106R (CAA→CGA)	PP_2031 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,308,753	0	T	C	5.7%	312.7 / 6.0	104	Q106R (CAA→CGA)	PP_2031	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (43/55); new base C (3/3); total (46/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 107 total aligned reads displayed.

TCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACGCGCACCTGGTCGAAGTC  >  NC_002947/2308605‑2308898

tCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGtt                                                                                                                                                    <  3:97214/149‑1 (MQ=255)

  ggggCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTg                                                                                                                                                   <  4:152486/148‑1 (MQ=255)

  ggggCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGCCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTg                                                                                                                                                   <  5:310273/148‑1 (MQ=255)

     gCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCtt                                                                                                                                               <  7:245016/149‑1 (MQ=255)

     gCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCtt                                                                                                                                               <  6:67411/149‑1 (MQ=255)

       ttGATTTCGTGGTGTTCGATGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTg                                                                                                                                             >  2:144693/1‑149 (MQ=255)

         gATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTg                                                                                                                                             <  2:344194/147‑1 (MQ=255)

         gATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGc                                                                                                                                            <  7:94825/148‑1 (MQ=255)

         gATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCt                                                                                                                                           >  1:152846/1‑149 (MQ=255)

           tttCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGc                                                                                                                                                 <  7:16065/141‑1 (MQ=255)

           tttCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGc                                                                                                                                                 >  8:16065/1‑141 (MQ=255)

           tttCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTcg                                                                                                                                         >  4:219021/1‑149 (MQ=255)

              cGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTcgac                                                                                                                                       <  4:15197/148‑1 (MQ=255)

               gtggtgTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACACCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTcgac‑ga                                                                                                                                    <  2:152846/149‑1 (MQ=255)

                  gtgtTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCAACGGCTTTTCttcg                                                                                                                                        <  3:78526/143‑4 (MQ=255)

                  gtgtTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCAACGGCTTTTCtacg                                                                                                                                        >  4:78526/1‑140 (MQ=255)

                   tgtTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGAt                                                                                                                                >  5:156230/1‑149 (MQ=255)

                    gtTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGAt                                                                                                                                >  2:328866/1‑148 (MQ=255)

                       cGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGaa                                                                                                                             <  1:328866/148‑1 (MQ=255)

                       cGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATAAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATg                                                                                                                               >  1:140400/1‑146 (MQ=255)

                       cGTTGTAGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATg                                                                                                                               >  7:227461/1‑146 (MQ=255)

                        gTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCGCGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGa                                                                                                                                 >  3:318857/1‑143 (MQ=255)

                        gTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCGCGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGa                                                                                                                                 <  4:318857/143‑1 (MQ=255)

                        gTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATg                                                                                                                               >  5:348119/1‑145 (MQ=255)

                           gTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGtt                                                                                                                                                    >  8:308527/1‑122 (MQ=255)

                           gTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGtt                                                                                                                                                    <  7:308527/122‑1 (MQ=255)

                             cGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGc                                                                                                                      <  2:329298/149‑1 (MQ=255)

                              gCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGcc                                                                                                                     >  7:293135/1‑149 (MQ=255)

                                cGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCAGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCg                                                                                                                    >  8:127534/1‑148 (MQ=255)

                                 gAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCt                                                                                                                  <  5:213831/149‑1 (MQ=255)

                                  aaCACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTc                                                                                                                 <  5:69432/149‑1 (MQ=255)

                                    cacCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTcgc                                                                                                               <  8:243683/149‑1 (MQ=255)

                                          gccgccGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGttcat                                                                                                         >  2:353568/1‑147 (MQ=255)

                                            cgccgATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCg                                                                                                       >  2:249387/1‑149 (MQ=255)

                                            cgccgATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCg                                                                                                       <  1:140562/149‑1 (MQ=255)

                                              ccgATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAGGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTg                                                                                                     >  1:136549/1‑149 (MQ=255)

                                               cgATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTgg                                                                                                    <  7:127534/149‑1 (MQ=255)

                                               cgATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTgg                                                                                                    <  1:144693/149‑1 (MQ=255)

                                                 aTGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGACGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGaa                                                                                                  <  2:136549/149‑1 (MQ=255)

                                                     ggTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGc                                                                                              <  4:168738/149‑1 (MQ=255)

                                                        cGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTg                                                                                           <  7:169442/149‑1 (MQ=255)

                                                         gTTGTGGCCGATCGCTTCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTg                                                                                           <  8:227461/148‑1 (MQ=255)

                                                            gtgGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGgtgt                                                                                       >  5:93298/1‑149 (MQ=255)

                                                            gtgGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGgtgt                                                                                       >  6:8788/1‑149 (MQ=255)

                                                             tgGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGTAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGtgtg                                                                                      <  8:106833/149‑1 (MQ=255)

                                                             tgGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGtgtg                                                                                      <  6:156230/149‑1 (MQ=255)

                                                             tgGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGtgtg                                                                                      <  5:215727/149‑1 (MQ=255)

                                                              ggCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCAACGGCTTTTCttc                                                                                                                                         <  3:319599/98‑3 (MQ=255)

                                                              ggCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCAACGGCTTTTCttc                                                                                                                                         >  4:319599/1‑96 (MQ=255)

                                                               gCCCTGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGTGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGc                                                                                     <  8:128779/148‑1 (MQ=255)

                                                               gCCCAGCGCTGCCCAGCCGCACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCg                                                                                    >  2:213232/1‑149 (MQ=255)

                                                               gCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCg                                                                                    >  4:44336/1‑149 (MQ=255)

                                                                  cAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAgg                                                                                                                                  <  7:258207/100‑1 (MQ=255)

                                                                  cAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAgg                                                                                                                                  >  8:258207/1‑100 (MQ=255)

                                                                    gcgcTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTcc                                                                               <  5:202358/149‑1 (MQ=255)

                                                                            cAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTAcg                                                                       >  4:269805/1‑149 (MQ=255)

                                                                              gCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTAcgcg                                                                     >  8:14761/1‑149 (MQ=255)

                                                                               ccGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCTAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACgcgc                                                                    <  5:119392/149‑1 (MQ=255)

                                                                                cGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCa                                                                   <  6:348119/149‑1 (MQ=255)

                                                                                cGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGGGTGCGGCTCCTGGGTACGCGCa                                                                   <  1:353568/149‑1 (MQ=255)

                                                                                  aaCAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGt                                                                 >  8:232715/1‑149 (MQ=255)

                                                                                    cAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGa                                                               <  8:1122/149‑1 (MQ=255)

                                                                                    cAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGCCTCCTGGGTACGCGCAGTGa                                                               >  8:68643/1‑149 (MQ=255)

                                                                                     aGCGCCGCAGCCTGGGCCTCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGac                                                              <  6:311803/149‑1 (MQ=255)

                                                                                         ccgcAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTg                                                                                           >  2:230078/1‑116 (MQ=255)

                                                                                         ccgcAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTg                                                                                           <  1:230078/116‑1 (MQ=255)

                                                                                          cgcAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGc                                                          >  2:284481/1‑148 (MQ=255)

                                                                                             aGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCa                                                        >  5:363145/1‑147 (MQ=255)

                                                                                             aGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCa                                                        <  6:363145/147‑1 (MQ=255)

                                                                                              gCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTc                                                     <  3:269805/149‑1 (MQ=255)

                                                                                              gCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTc                                                     <  7:232715/149‑1 (MQ=255)

                                                                                               ccTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTg                                                                                           >  4:27897/1‑110 (MQ=255)

                                                                                               ccTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTg                                                                                           <  3:27897/110‑1 (MQ=255)

                                                                                                  gggCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGc                                                                                     <  5:70560/113‑1 (MQ=255)

                                                                                                     ccgccAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCCTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCAAGTCGATGTTg                                              >  5:91929/1‑149 (MQ=255)

                                                                                                      cgccAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTCGGCTTTGCTCGACATAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTg                                              >  1:102687/1‑149 (MQ=255)

                                                                                                       gccAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTgcg                                            <  3:219021/149‑1 (MQ=255)

                                                                                                        ccAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTgcgc                                           <  7:291881/149‑1 (MQ=255)

                                                                                                         cAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGca                                          <  8:293135/149‑1 (MQ=255)

                                                                                                             aCGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCAcatc                                      <  1:284481/149‑1 (MQ=255)

                                                                                                             aCGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCAcatc                                      <  3:44336/149‑1 (MQ=255)

                                                                                                               ggACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCAcatcat                                    >  2:73782/1‑149 (MQ=255)

                                                                                                                 aCTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGt                                                                 <  4:240178/118‑1 (MQ=255)

                                                                                                                 aCTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGt                                                                 >  3:240178/1‑118 (MQ=255)

                                                                                                                     gAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCgg                              >  3:159753/1‑149 (MQ=255)

                                                                                                                        cATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCt                           <  6:93298/149‑1 (MQ=255)

                                                                                                                         aTCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTg                          >  6:23994/1‑149 (MQ=255)

                                                                                                                             gCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCgg                                                                                                                       <  8:258926/52‑1 (MQ=255)

                                                                                                                             gCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCgg                                                                                                                       >  7:258926/1‑52 (MQ=255)

                                                                                                                             gCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTa                       >  2:250335/1‑148 (MQ=255)

                                                                                                                             gCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTa                       >  1:130708/1‑148 (MQ=255)

                                                                                                                                   aGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGGACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACgcgc                <  5:8788/149‑1 (MQ=255)

                                                                                                                                    gCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACGCGCa               <  1:93031/149‑1 (MQ=255)

                                                                                                                                    gCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGGTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACGCGCa               <  1:88323/149‑1 (MQ=255)

                                                                                                                                       gaagaaAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTg                          <  5:27949/135‑1 (MQ=255)

                                                                                                                                       gaagaaAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTg                          >  6:27949/1‑135 (MQ=255)

                                                                                                                                              aGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACGCGCACCTGGTCGaa     >  4:144187/1‑149 (MQ=255)

                                                                                                                                              aGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTCGCGCACATCATCGCCGGCCTGGTAAACGCGCACCTGGTCGaa     <  1:249387/149‑1 (MQ=255)

                                                                                                                                                ccGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCATTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACGCGCACCTGGTCGAAGt   <  4:154157/149‑1 (MQ=255)

                                                                                                                                                  gTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACGCGCACCTGGTCGAAGTc  >  1:345214/1‑148 (MQ=255)

TCGGGGCTTGATTTCGTGGTGTTCGTTGTCGCCGAACACCAGGCCGCCGATGCGGTCGTTGTGGCCCAGCGCTGCCCAGCCGAACAGCGCCGCAGCCTGGGCCGCCAGCACGGACTTGAACATCAGCCCTGAGCCGAAGAAAAGCCGTTGGCTTTGCTCGAC‑GAGGATGAAGATCGGCCGCTCGCGTTCTTCGTGGAACAGCTTGGTGTGCGGCTCCTGGGTACGCGCAGTGACACGCCAGTCGATGTTGCGCACATCATCGCCGGCCTGGTAAACGCGCACCTGGTCGAAGTC  >  NC_002947/2308605‑2308898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`