Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,345,326 C→A 6.9% G8* (GGA→TGA) ‡ PP_2062 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,345,3260CA 6.9% 242.8 / 5.9 82G8* (GGA→TGA) ‡PP_2062hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (42/34);  new base A (3/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TAGTTTGCTGGCTGGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGA  >  NC_002947/2345178‑2345471
                                                                                                                                                     |                                                                                                                                                 
tAGTTTGCTGGCTGGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTcc                                                                                                                                                   >  4:281835/1‑149 (MQ=255)
      gctggctgGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGt                                                                                                                                                 >  4:89314/1‑145 (MQ=255)
         ggctggcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCt                                                                                                                                          <  3:89314/149‑1 (MQ=255)
           ctggcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTgg                                                                                                                                        <  1:194324/149‑1 (MQ=255)
           ctggcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTgg                                                                                                                                        <  1:195945/149‑1 (MQ=255)
              gcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACGGAGTCAGAtatatgt                                                                                                                                      <  5:114001/148‑7 (MQ=255)
               ccTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACTGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACt                                                                                                                                    >  4:166/1‑149 (MQ=255)
                cTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACtt                                                                                                                                   <  3:281835/149‑1 (MQ=255)
                cTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACGGAGTCAGAtatatg                                                                                                                                       <  4:315683/145‑6 (MQ=255)
                cTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACGGAGTCAGAtatatg                                                                                                                                       >  3:315683/1‑140 (MQ=255)
                   ttgttgGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACTGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTg                                                                                                                                  <  3:166/147‑1 (MQ=255)
                      ttgGGGCCGTGGATGTTCGTGCGGCGACATGTTGGTCACGGGCCTGGCGTTCCAAACGGGGTGCAATGGTCGGAGCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCAt                                                                                                                              <  2:273991/148‑1 (MQ=255)
                         gggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAg                                                                                                                           <  7:289969/148‑1 (MQ=255)
                         gggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAg                                                                                                                           >  8:289969/1‑148 (MQ=255)
                         gggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGt                                                                                                                          >  1:284895/1‑149 (MQ=255)
                          ggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTg                                                                                                                         <  7:90208/149‑1 (MQ=255)
                            ccGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGAt                                                                                                                       >  4:100597/1‑149 (MQ=255)
                                    gTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCAt                                                                                                                >  3:275527/1‑148 (MQ=255)
                                             gcgcCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCg                                                                                                      <  3:100597/149‑1 (MQ=255)
                                              cgcCATGTTGGTCACGGACCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGt                                                                                                     <  2:145931/149‑1 (MQ=255)
                                                ccATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTAGTGc                                                                                                   >  3:248331/1‑149 (MQ=255)
                                                     gtGGGCACGGGCCTGGCCTTCCAAACGGGCAGCACTCGGCGCATCGTGTATTTTACGGGGTGTCGA‑AGGTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGttt                                                                                              <  8:258375/148‑1 (MQ=255)
                                                       ggTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTc                                                                                             >  7:213456/1‑148 (MQ=255)
                                                         tCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGAc                                                                                          >  2:103754/1‑149 (MQ=255)
                                                           aCGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACg                                                                                         <  1:103754/148‑1 (MQ=255)
                                                             gggCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTg                                                                                                 <  7:336514/138‑1 (MQ=255)
                                                             gggCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTg                                                                                                 >  8:336514/1‑138 (MQ=255)
                                                               gCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCttt                                                                                    >  8:124706/1‑149 (MQ=255)
                                                               gCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCttt                                                                                    >  8:37060/1‑149 (MQ=255)
                                                                ccTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGAGAGTTTTATGCTTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCAATTAGCCATTCGTGCTGTTTCGACGTCttt                                                                                    >  1:98051/1‑149 (MQ=255)
                                                                 cTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTc                                                                                  >  2:92232/1‑149 (MQ=255)
                                                                  tGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCg                                                                                 >  8:120703/1‑149 (MQ=255)
                                                                  tGGCTATACAAAATGGGTGCAATGGTCGGATCGTGTTTTTTACGGGTTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCAACAGTGATTACCCATTAGAAATACGTGCTGTTTCGAAGTCATTTc                                                                                  >  8:120705/1‑148 (MQ=255)
                                                                   ggCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGc                                                                                >  5:89978/1‑149 (MQ=255)
                                                                      tttCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGc                                                                             >  4:219655/1‑149 (MQ=255)
                                                                      tttCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGc                                                                             <  6:277358/149‑1 (MQ=255)
                                                                           aaaCGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTg                                                                                                    >  4:245415/1‑121 (MQ=255)
                                                                           aaaCGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTg                                                                                                    <  3:245415/121‑1 (MQ=255)
                                                                           aaaCGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAgg                                                                         >  1:303563/1‑148 (MQ=255)
                                                                           aaaCGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGc                                                                        >  2:208832/1‑149 (MQ=255)
                                                                                     cAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCtt                                                              <  8:213456/149‑1 (MQ=255)
                                                                                      aaTGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCtt                                                              <  3:219655/148‑1 (MQ=255)
                                                                                        tGGACGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCAGTCAGATCTGGAACTTTGAATCAGTGATTACCAATTAGCCATTCGTGATTTTTCGACGTGTTTTCGATGACAGGCCTGCAGGCTTCa                                                            >  8:129862/1‑148 (MQ=255)
                                                                                         ggTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTc                                                             <  2:275827/146‑1 (MQ=255)
                                                                                         ggTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTc                                                             >  1:275827/1‑146 (MQ=255)
                                                                                          gTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGGCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCaaa                                                          <  4:248331/148‑1 (MQ=255)
                                                                                          gTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCaaa                                                          <  1:92232/148‑1 (MQ=255)
                                                                                          gTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCaaa                                                          <  2:284895/148‑1 (MQ=255)
                                                                                             ggATCGTGTTTTTTACGGGGTGTCGA‑AGATTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGAACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAaagg                                                        >  1:202184/1‑147 (MQ=255)
                                                                                             gcaTCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAaaggaa                                                      <  4:298642/147‑1 (MQ=255)
                                                                                              gATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCtttt                                                                                   >  8:261298/1‑119 (MQ=255)
                                                                                              gATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCtttt                                                                                   <  7:261298/119‑1 (MQ=255)
                                                                                                  gtgtagtttACGGGGGGGCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGa                                                 <  4:275527/143‑1 (MQ=255)
                                                                                                  gtgtTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGa                                                 <  7:37060/149‑1 (MQ=255)
                                                                                                     ttttttACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGtt                                              >  2:293617/1‑149 (MQ=255)
                                                                                                     ttttttAAGAGGTTTCGA‑ACTTTTATGATTTCAAATATATCTGACTCCGTCAGATCTGCCAAATTTAATCAGTTATTACCCATTAGACATTCTTGATGTTTAGACTTCTTTTCTCTCCAAATCCTGAATGCTTCAaaggaatttaatt                                               >  4:23795/1‑141 (MQ=255)
                                                                                                         ttACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCt                                           <  7:124706/148‑1 (MQ=255)
                                                                                                           aCGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTg                                          >  3:110452/1‑147 (MQ=255)
                                                                                                            cGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTa                                        <  2:98051/148‑1 (MQ=255)
                                                                                                             gtgttgtAGA‑AGTTTTATGCTTTGAAATATATCTTACTCCGTCAGATCTGGCACTTGGAATCAGTGATTACCCATTAGCCATTCGTTCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAg                                       <  7:129862/144‑1 (MQ=255)
                                                                                                             ggggTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGt                                      >  1:132739/1‑149 (MQ=255)
                                                                                                               ggTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTcc                                    >  3:134059/1‑149 (MQ=255)
                                                                                                                    cGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGccc                               >  8:21709/1‑149 (MQ=255)
                                                                                                                     gA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGccc                               >  6:219131/1‑148 (MQ=255)
                                                                                                                     gA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTAGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTATTCCTGacc                               >  8:303036/1‑148 (MQ=255)
                                                                                                                         gTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATc                           <  2:303563/149‑1 (MQ=255)
                                                                                                                             tATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGAATACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCttt                        <  1:293617/148‑1 (MQ=255)
                                                                                                                                gCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCAc                     <  4:134059/148‑1 (MQ=255)
                                                                                                                                 cGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCAATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTg                   >  2:270351/1‑149 (MQ=255)
                                                                                                                                    tGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGc                                 <  6:96568/132‑1 (MQ=255)
                                                                                                                                    tGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGc                                 >  5:96568/1‑132 (MQ=255)
                                                                                                                                       cATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAgg              >  7:136793/1‑148 (MQ=255)
                                                                                                                                           gatCTGACGGAGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAg               <  7:97452/142‑1 (MQ=255)
                                                                                                                                           gatCTGACGGAGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAg               >  8:97452/2‑143 (MQ=255)
                                                                                                                                            atCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATc          >  1:161954/1‑147 (MQ=255)
                                                                                                                                               tGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGc     >  4:128906/1‑149 (MQ=255)
                                                                                                                                               tGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGc     >  2:295257/1‑149 (MQ=255)
                                                                                                                                                gACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCg    >  1:159762/1‑149 (MQ=255)
                                                                                                                                                 aCTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAg       <  7:21709/145‑1 (MQ=255)
                                                                                                                                                   tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCgg   >  2:65526/1‑147 (MQ=255)
                                                                                                                                                   tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCgg   <  1:65526/147‑1 (MQ=255)
                                                                                                                                                   tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGACCGATCTTTCACTGCCACGGATCAAGGCGGa  >  3:255733/1‑148 (MQ=255)
                                                                                                                                                   tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCAATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCGTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGa  >  6:99552/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TAGTTTGCTGGCTGGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTACGGGGTGTCGA‑AGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGA  >  NC_002947/2345178‑2345471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑