BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,378,690	A→T	5.1%	N82I (AAT→ATT)	PP_2086 →	CrfX family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,378,690	0	A	T	5.1%	300.4 / 3.5	97	N82I (AAT→ATT)	PP_2086	CrfX family protein
Reads supporting (aligned to +/- strand): ref base A (44/48); new base T (2/3); total (46/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 105 total aligned reads displayed.

CGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCGTGGTGCTGCTCGGTTTCG  >  NC_002947/2378544‑2378838

cgTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATg                                                                                                                                                    <  8:294863/149‑1 (MQ=255)

   gCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCATTgg                                                                                                                                                   >  8:260725/1‑147 (MQ=255)

     tGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgcag                                                                                                                                                >  4:180967/1‑148 (MQ=255)

        aaaCCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgcagca                                                                                                                                              >  8:303641/1‑147 (MQ=255)

        aaaCCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCaaa                                                                                                                                            >  6:337079/1‑149 (MQ=255)

        aaaCCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCaaa                                                                                                                                            <  3:192837/149‑1 (MQ=255)

         aaccgccaaccgccAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAg                                                                                                                                           >  2:77395/1‑149 (MQ=255)

          accgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCATTGgcagcgttgc                                                                                                                                          <  7:260725/149‑6 (MQ=255)

          accgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGc                                                                                                                                          <  6:85621/149‑1 (MQ=255)

          accgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGc                                                                                                                                          <  3:135404/149‑1 (MQ=255)

           ccgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCATTGgcagcgttgcg                                                                                                                                         <  4:251002/149‑7 (MQ=255)

           ccgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCATTGgcagcgttgcg                                                                                                                                         <  7:220834/149‑7 (MQ=255)

           ccgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCa                                                                                                                                         <  6:256141/149‑1 (MQ=255)

           ccgccaaccgccaGGTTGGCGCGGGTGATCTG‑TCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCGTCCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCa                                                                                                                                         <  1:85397/149‑1 (MQ=255)

            cgccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAg                                                                                                                                           >  3:302672/1‑146 (MQ=255)

             gccaaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGa                                                                                                                                       <  1:164733/149‑1 (MQ=255)

                aaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGAt                                                                                                                                      >  1:36464/1‑147 (MQ=255)

                aaccgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATaa                                                                                                                                    <  7:128422/149‑1 (MQ=255)

                   cgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGc                                                                                                                                 >  6:285116/1‑149 (MQ=255)

                   cgccaGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGc                                                                                                                                 >  3:107261/1‑149 (MQ=255)

                        ggTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGAtt                                                                                                                            <  5:337079/149‑1 (MQ=255)

                             gcgcGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAata                                                                                                                       <  3:180967/149‑1 (MQ=255)

                             gcgcGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAat                                                                                                                        <  5:80745/148‑1 (MQ=255)

                                    tGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACt                                                                                                                <  1:39741/149‑1 (MQ=255)

                                           ttCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCaa                                                                                                                                                      <  8:198883/104‑1 (MQ=255)

                                           ttCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCaa                                                                                                                                                      >  7:198883/1‑104 (MQ=255)

                                           ttCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtctc                                                                                                         >  7:62905/1‑149 (MQ=255)

                                           ttCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtctc                                                                                                         <  2:109434/149‑1 (MQ=255)

                                           ttCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGAtc                                                                                                           <  1:334755/147‑1 (MQ=255)

                                             cAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATctct                                                                                                        <  5:255439/148‑1 (MQ=255)

                                             cAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTg                                                                                                       >  8:168196/1‑149 (MQ=255)

                                              aGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATaa                                                                                                                                    <  8:186140/119‑1 (MQ=255)

                                              aGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATaa                                                                                                                                    >  7:186140/1‑119 (MQ=255)

                                              aGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTg                                                                                                       <  5:227306/148‑1 (MQ=255)

                                                ccTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGgc                                                                                                                                                  <  7:145138/103‑1 (MQ=255)

                                                ccTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGAc                                                                                                    >  2:239763/1‑149 (MQ=255)

                                                ccTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGAc                                                                                                    <  1:142259/149‑1 (MQ=255)

                                                 cTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGAcc                                                                                                   <  7:91756/149‑1 (MQ=255)

                                                   gCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTAGGCGCATGTTGCG‑CCGGTGCGTCGACACTCATCCCGCAACGCTGCAAATGGCAGCAAAGCAGAAAAGGACGATAGAATATGGAACTCGATCTCTGGAcc                                                                                                   >  8:33347/1‑147 (MQ=255)

                                                       ggCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGGTCTCTGGACCCagag                                                                                              <  4:57751/148‑1 (MQ=255)

                                                         ccGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACt                                                                                                                >  8:149167/1‑128 (MQ=255)

                                                         ccGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACt                                                                                                                <  7:149167/128‑1 (MQ=255)

                                                          cGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTg                                                                                          <  1:116621/149‑1 (MQ=255)

                                                           gCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTgg                                                                                         >  4:179074/1‑149 (MQ=255)

                                                            cTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGt                                                                                        <  7:168196/149‑1 (MQ=255)

                                                                  ccAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTg                                                                                                                           >  2:8601/1‑108 (MQ=255)

                                                                  ccAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTg                                                                                                                           >  6:192597/1‑108 (MQ=255)

                                                                  ccAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAat                                                                                                                        <  6:65913/111‑1 (MQ=255)

                                                                  ccAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAat                                                                                                                        >  5:65913/1‑111 (MQ=255)

                                                                  caaGGCTCTGCTGATTGCCGTGAACAATGGGTCGGCGGATGTTGGG‑CCGGTGGGTCGACTCTCTTCGCGCAACTCTGCCTATGGCAGCAAAGCAGATAAGTGCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACcgc                                                                                  <  7:33347/147‑1 (MQ=255)

                                                                    aGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGGATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGa                                                                                >  7:84727/1‑149 (MQ=255)

                                                                    aGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGa                                                                                <  5:229072/149‑1 (MQ=255)

                                                                     ggCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGGATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGAt                                                                               <  8:84727/149‑1 (MQ=255)

                                                                       cgcTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGa                                                                             <  2:36464/149‑1 (MQ=255)

                                                                        gctgctGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGAc                                                                            <  1:77395/149‑1 (MQ=255)

                                                                            ctgATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTg                                                                          <  1:239763/147‑1 (MQ=255)

                                                                            ctgATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGc                                                                         <  1:349183/148‑1 (MQ=255)

                                                                             tgATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGccc                                                                       >  3:278725/1‑149 (MQ=255)

                                                                                 attgctGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCtt                                                                     <  5:24951/142‑1 (MQ=255)

                                                                                   ccGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTgg                                                                  <  5:285116/148‑1 (MQ=255)

                                                                                   ccGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGa                                                                 <  4:107261/149‑1 (MQ=255)

                                                                                     gTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGAc                                                                >  1:307847/1‑148 (MQ=255)

                                                                                         aCAATGGCTCGGCGCATGTTGGG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGATCACCGCGATGACTGCCCTTTGGACCAAg                                                            <  6:20431/148‑1 (MQ=255)

                                                                                           aaTGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTa                                                         <  1:279701/149‑1 (MQ=255)

                                                                                             tGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGc                                                       >  6:138005/1‑149 (MQ=255)

                                                                                                      gcATGTTGCG‑CCGGTGCGTCGACACTCTTACCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGAATGAATATGGAACTCGATCTCTGGACACAGACCCTGGTCACCGCGATGACTGCCCTTAGGACAAAGGTAGCGAACTTCAt                                              >  8:290984/1‑149 (MQ=255)

                                                                                                         tGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATccc                                           >  8:133335/1‑149 (MQ=255)

                                                                                                          gTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACTCTGCCAATGGCAGCAAAGAAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGACTGCTCACCGCGATGACTGCCCTTTGTACAAAGGTAGCGAACTTCATccc                                           >  6:332125/1‑148 (MQ=255)

                                                                                                              cg‑cCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAAc                                       <  5:138005/148‑1 (MQ=255)

                                                                                                              cg‑cCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAAc                                       <  8:62905/148‑1 (MQ=255)

                                                                                                                 ccGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTg                                    >  2:7634/1‑149 (MQ=255)

                                                                                                                  cGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGt                                   >  2:14012/1‑149 (MQ=255)

                                                                                                                    gTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCg                                                      >  2:197789/1‑128 (MQ=255)

                                                                                                                    gTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCg                                                      <  1:197789/128‑1 (MQ=255)

                                                                                                                    gTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTc                                 <  4:302672/149‑1 (MQ=255)

                                                                                                                    gTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTc                                 <  8:262919/149‑1 (MQ=255)

                                                                                                                            acacTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGATCTCGATCTCTGGACCCAGAGGCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCt                         <  5:332125/149‑1 (MQ=255)

                                                                                                                            acacTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGa                                                                 <  6:85533/109‑1 (MQ=255)

                                                                                                                            acacTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGa                                                                 >  5:85533/1‑109 (MQ=255)

                                                                                                                            acacTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCACTTTGGACCAAGGTAGCGAACTTCATCCCCAACATGTTCGGCGCGCt                         >  6:284187/1‑149 (MQ=255)

                                                                                                                             cacTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTg                        >  8:19976/1‑149 (MQ=255)

                                                                                                                                  ttCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCATTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCgt                   >  2:116379/1‑149 (MQ=255)

                                                                                                                                    cccGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCgtgg                 >  4:70749/1‑149 (MQ=255)

                                                                                                                                     ccGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGgcgcgc                          >  3:22435/1‑139 (MQ=255)

                                                                                                                                     ccGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGgcgcgc                          <  4:22435/139‑1 (MQ=255)

                                                                                                                                     ccGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCgtggt                >  6:84510/1‑149 (MQ=255)

                                                                                                                                      cGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTgg                                                                  >  5:339225/1‑98 (MQ=255)

                                                                                                                                      cGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTgg                                                                  <  6:339225/98‑1 (MQ=255)

                                                                                                                                      cGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGc                                                       >  3:174517/1‑109 (MQ=255)

                                                                                                                                      cGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGc                                                       <  4:174517/109‑1 (MQ=255)

                                                                                                                                         tttgCTGCCATTGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCGTGGtgctg            >  2:232580/4‑149 (MQ=255)

                                                                                                                                         aaCGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCGTGGtgctg            >  3:206306/1‑149 (MQ=255)

                                                                                                                                              tGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCGTGGTGCTGCTCgg       >  1:105653/1‑149 (MQ=255)

                                                                                                                                                ccAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCGTGGTGCTGCTCGGtt     >  8:129713/1‑149 (MQ=255)

                                                                                                                                                  aaTGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTg                        <  6:255185/128‑1 (MQ=255)

                                                                                                                                                  aaTGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGACCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTg                        >  5:255185/1‑128 (MQ=255)

                                                                                                                                                   aTGGCAGCAAAGCATATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCgtg                  <  4:214553/133‑1 (MQ=255)

                                                                                                                                                   aTGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATc                                             <  8:119456/106‑1 (MQ=255)

                                                                                                                                                   aTGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATc                                             >  7:119456/1‑106 (MQ=255)

                                                                                                                                                   aTGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGAGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCGTGGTGCTGCTCGGTTTCg  >  5:79946/1‑149 (MQ=255)

CGTGCTGAAAACCGCCAACCGCCAGGTTGGCGCGGGTGATCTGTTCAGCCTGCTTGGCCGCTGGAGCCAGGCGCTGCTGATTGCCGTGAACAATGGCTCGGCGCATGTTGCG‑CCGGTGCGTCGACACTCTTCCCGCAACGCTGCCAATGGCAGCAAAGCAGATAAGGCCGATTGAATATGGAACTCGATCTCTGGACCCAGAGCCTGGTCACCGCGATGACTGCCCTTTGGACCAAGGTAGCGAACTTCATCCCCAACCTGTTCGGCGCGCTGGTCGTGGTGCTGCTCGGTTTCG  >  NC_002947/2378544‑2378838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`