BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,508,898	A→G	5.5%	C73R (TGC→CGC)	PP_2203 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,508,898	0	A	G	5.5%	322.0 / 4.6	108	C73R (TGC→CGC)	PP_2203	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (54/47); new base G (4/2); total (58/50)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAT  >  NC_002947/2508756‑2509045

tCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCacgacg                                                                                                                                                >  2:62484/1‑148 (MQ=255)

  aCCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGc                                                                                                                                               >  3:3948/1‑147 (MQ=255)

  aCCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGc                                                                                                                                               >  6:39466/1‑147 (MQ=255)

  aCCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACATGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCAGCCGATCACCCCG‑GTGTGGCACGACGc                                                                                                                                               >  6:225319/1‑147 (MQ=255)

    ccATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCaacg                                                                                                                                           >  5:307329/1‑147 (MQ=255)

     cATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGc                                                                                                                                          >  2:32286/1‑149 (MQ=255)

      aTCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCg                                                                                                                                         >  1:233452/1‑149 (MQ=255)

           cATCCCGGGCGAGCCCATGGGCTTGCACGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCTATCACCCCG‑GTGTGGCACGACGCAAGGCGTgg                                                                                                                                      <  8:161009/147‑1 (MQ=255)

                 gggCGAGCCCATGGGCATGCTCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGc                                                                                                                              >  7:205122/1‑149 (MQ=255)

                 gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGc                                                                                                                                          <  1:209875/137‑1 (MQ=255)

                 gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGAGCCACGc                                                                                                                              >  6:35341/1‑149 (MQ=255)

                 gggCCACCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGGCGTTGCGGTTGGCCAGCATGC‑GCACCTGCGCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGCGTGTGGCACGACGCAAGGCGTGgcacaccg                                                                                                                               <  1:32286/149‑7 (MQ=255)

                     gAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAg                                                                                                                          >  3:139798/1‑149 (MQ=255)

                         ccttGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                      <  2:233452/146‑1 (MQ=255)

                         ccATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                      >  3:66302/1‑149 (MQ=255)

                         ccAGGGGCAGGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCAGGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                      <  5:99144/149‑1 (MQ=255)

                          cATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCacga                                                                                                                                                  <  3:115540/120‑1 (MQ=255)

                                cATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGa                                                                                                               <  1:260452/149‑1 (MQ=255)

                                    cccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGgc                                                                                                           >  2:44729/1‑149 (MQ=255)

                                        gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCGCCACGCCTTGCGTCGTGCCACACCggg                                                                                                                          <  8:106155/130‑3 (MQ=38)

                                        gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCGCCACGCCTTGCGTCGTGCCACACCggg                                                                                                                          >  7:106155/1‑128 (MQ=38)

                                        gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGt                                                                                                                                        <  1:295646/116‑1 (MQ=255)

                                        gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGt                                                                                                                                        >  2:295646/1‑116 (MQ=255)

                                          aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                     >  1:71909/1‑149 (MQ=255)

                                          aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                     >  3:321605/1‑149 (MQ=255)

                                          aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGGGGCACGACGCAAGGCGGGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                     <  1:22758/149‑1 (MQ=255)

                                             gCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGAAGGCGGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCTCG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCgggtt                                                                                                                        >  1:64573/1‑124 (MQ=21)

                                             gCCAGCCCCTTGAGGTCGTTGCGTTGGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCTCG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCggggt                                                                                                                        <  2:64573/127‑2 (MQ=21)

                                                aGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCCCCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTgg                                                                                               >  2:250873/1‑149 (MQ=255)

                                                aGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTgg                                                                                               <  6:170395/149‑1 (MQ=255)

                                                 ggCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGc                                                                                              <  2:111282/149‑1 (MQ=255)

                                                   tccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                            >  2:139326/2‑149 (MQ=255)

                                                    ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                            >  7:265024/1‑148 (MQ=255)

                                                    ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                            >  5:358614/1‑148 (MQ=255)

                                                    ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCg                                                                                           <  7:63081/149‑1 (MQ=255)

                                                        gAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                            <  4:128907/144‑1 (MQ=255)

                                                        gAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                            >  3:128907/1‑144 (MQ=255)

                                                        gAGGTCGTTGCGTTTAGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                 <  3:68895/139‑1 (MQ=255)

                                                        gAGGTCGTTGCGTTTAGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                 >  4:68895/1‑139 (MQ=255)

                                                         aGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                      <  5:39466/149‑1 (MQ=255)

                                                              gTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCg                                                                                  <  1:62484/148‑1 (MQ=255)

                                                                   gTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                            <  4:66302/149‑1 (MQ=255)

                                                                   gTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAAGTTTGGCTCGACGTGGTCGtgtccg                                                                            >  8:162505/1‑144 (MQ=255)

                                                                      cctacagcagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                            <  5:366217/142‑1 (MQ=255)

                                                                          cagcagGC‑GCACCTGCTCGGCCGGCCCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCCAGGCGTGGCGCCCCGCCGCGGCGTTGCTTGCCGGCGCTCAGGTGTGGCGCGACGGGGTCGGTGACGGTCAAAc                                                                     >  3:179108/1‑149 (MQ=255)

                                                                          cagcagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAAc                                                                     >  3:46567/1‑149 (MQ=255)

                                                                             cagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCGG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAt                                                                  <  8:205122/149‑1 (MQ=255)

                                                                              agGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAtt                                                                 <  7:293053/149‑1 (MQ=255)

                                                                                 c‑gcCCCTGCGCGGCCGGCCCGGGGCCGGCGACTCCCTGGCCGCCCCTCCCCCCG‑GGGGGGCCCGCCGCACGGCGGGGCGCCCCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGGCGTTGACGGTAAAACCATTGTc                                                              <  4:179108/149‑1 (MQ=255)

                                                                                    cACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCAc                                                            >  1:148113/1‑149 (MQ=255)

                                                                                       cTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACg                                                           >  4:114992/1‑147 (MQ=255)

                                                                                         gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                               >  1:81449/1‑125 (MQ=255)

                                                                                         gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                               <  2:81449/125‑1 (MQ=255)

                                                                                          cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                  >  4:193052/1‑89 (MQ=255)

                                                                                          cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                  <  3:193052/89‑1 (MQ=255)

                                                                                          cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGt                                                      <  2:148113/149‑1 (MQ=255)

                                                                                          cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGt                                                      <  3:114992/149‑1 (MQ=255)

                                                                                             ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTaa                                                                        >  7:281300/1‑128 (MQ=255)

                                                                                             ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTaa                                                                        <  8:281300/128‑1 (MQ=255)

                                                                                               ccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGa                                                                              <  4:58391/120‑1 (MQ=255)

                                                                                               ccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGa                                                                              >  3:58391/1‑120 (MQ=255)

                                                                                                cggcACGGGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGa                                                <  1:37953/149‑1 (MQ=255)

                                                                                                    aCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATc                                              >  2:77419/1‑147 (MQ=255)

                                                                                                     cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                           <  1:77419/149‑1 (MQ=255)

                                                                                                     cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                           <  4:139798/149‑1 (MQ=255)

                                                                                                      gTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAgg                                          >  4:141238/1‑149 (MQ=255)

                                                                                                          ccTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                      >  8:20235/1‑149 (MQ=255)

                                                                                                           cTTCGACTAACTTGGCGCCTTTCACCCGG‑GGGTGTCACGTCGCTAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                     <  5:225319/149‑1 (MQ=255)

                                                                                                           cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                      >  5:362025/1‑148 (MQ=255)

                                                                                                           cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                      <  6:362025/148‑1 (MQ=255)

                                                                                                           cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                     <  8:265024/149‑1 (MQ=255)

                                                                                                           cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                     >  2:340182/1‑149 (MQ=255)

                                                                                                           cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCGCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                     <  6:295971/149‑1 (MQ=255)

                                                                                                              cgacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                   <  1:340182/148‑1 (MQ=255)

                                                                                                               gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGATGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgcag                                  >  1:43345/1‑148 (MQ=255)

                                                                                                               gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                               >  7:187421/1‑135 (MQ=255)

                                                                                                               gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                               <  8:187421/135‑1 (MQ=255)

                                                                                                                           gccgATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgg                     >  3:123904/1‑149 (MQ=255)

                                                                                                                             cgATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgggg                   >  6:38901/1‑149 (MQ=255)

                                                                                                                              gATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGt                  >  6:39968/1‑149 (MQ=255)

                                                                                                                               aTCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCt                                                  >  4:247027/1‑116 (MQ=255)

                                                                                                                                tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                <  4:123904/149‑1 (MQ=255)

                                                                                                                                tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                <  4:46567/149‑1 (MQ=255)

                                                                                                                                  aCCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcgcg              >  4:171906/1‑149 (MQ=255)

                                                                                                                                   ccccG‑GTGTGGCACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg             >  4:87844/1‑149 (MQ=255)

                                                                                                                                    ctcG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg             >  2:267361/3‑148 (MQ=38)

                                                                                                                                    cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                      <  6:278/75‑1 (MQ=255)

                                                                                                                                    cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                      >  5:278/1‑75 (MQ=255)

                                                                                                                                    cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                   <  6:321991/126‑1 (MQ=255)

                                                                                                                                    cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                   >  5:321991/1‑126 (MQ=255)

                                                                                                                                    cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg             >  2:108621/1‑148 (MQ=255)

                                                                                                                                    cccG‑GTGTGGCACGACGCAAGGAGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg             >  7:53034/1‑148 (MQ=255)

                                                                                                                                      cG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg             >  5:367919/1‑146 (MQ=255)

                                                                                                                                      cG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg             >  8:276155/1‑146 (MQ=255)

                                                                                                                                      cG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg             >  6:306708/1‑146 (MQ=38)

                                                                                                                                          tgtgGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACg       >  4:220398/1‑149 (MQ=255)

                                                                                                                                              gCACGACGCAAGGCTTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGc                                                   <  7:242818/101‑1 (MQ=255)

                                                                                                                                              gCACGACGAAATGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGACGTAGACGGTAAAACCATTGTCACTCAGGTGGc                                                   >  8:242818/1‑101 (MQ=255)

                                                                                                                                               cACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt  <  3:87844/149‑1 (MQ=255)

                                                                                                                                               cACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt  >  3:333077/1‑149 (MQ=255)

TCACCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAT  >  NC_002947/2508756‑2509045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`