BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,508,905	A→C	6.3%	L70L (CTT→CTG) ‡	PP_2203 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,508,905	0	A	C	6.3%	316.8 / 7.9	104	L70L (CTT→CTG) ‡	PP_2203	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (49/47); new base C (5/2); total (54/50)
Fisher's exact test for biased strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 108 total aligned reads displayed.

CCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGG  >  NC_002947/2508759‑2509053

cccATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAg                                                                                                                                                    <  7:179294/149‑1 (MQ=255)

 ccATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCaacg                                                                                                                                                   >  5:307329/1‑147 (MQ=255)

   aTCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCg                                                                                                                                                 >  1:233452/1‑149 (MQ=255)

        cATCCCGGGCGAGCCCATGGGCTTGCACGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCTATCACCCCG‑GTGTGGCACGACGCAAGGCGTgg                                                                                                                                              <  8:161009/147‑1 (MQ=255)

              gggCGAGCCCATGGGCATGCTCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGc                                                                                                                                      >  7:205122/1‑149 (MQ=255)

              gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGc                                                                                                                                                  <  1:209875/137‑1 (MQ=255)

              gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGc                                                                                                                                                  >  2:209875/1‑137 (MQ=255)

              gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGAGCCACGc                                                                                                                                      >  6:35341/1‑149 (MQ=255)

              gggCCACCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGGCGTTGCGGTTGGCCAGCATGCGCACCTGCGCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGCGTGTGGCACGACGCAAGGCGTGgcacaccg                                                                                                                                       <  1:32286/149‑7 (MQ=255)

                  gAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAg                                                                                                                                  >  3:139798/1‑149 (MQ=255)

                      ccttGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                              <  2:233452/146‑1 (MQ=255)

                      ccATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                              >  3:66302/1‑149 (MQ=255)

                      ccAGGGGCAGGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCAGGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                              <  5:99144/149‑1 (MQ=255)

                           ggattGCCCGGCACCGCCCGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACTAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCtt                                                                                                                         <  8:123958/145‑1 (MQ=255)

                             cATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGa                                                                                                                       <  1:260452/149‑1 (MQ=255)

                              ttGCCGGGCACCGCCAGGCCTTTGTGGGGGTTGGGTTTGGCCTGCAGGCGCACCTGCTCGGCCGGCAGGTGGCCTTCGACTAACTTGCCGCCTATCACCCCG‑GTGTGGCAGGACGCACGGCGTGGCGCAACGCGGAGGGGTTGCTTGAc                                                                                                                      <  6:290831/148‑1 (MQ=255)

                                 cccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGgc                                                                                                                   >  2:44729/1‑149 (MQ=255)

                                 cccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGgc                                                                                                                   >  5:34866/1‑149 (MQ=255)

                                     gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCGCCACGCCTTGCGTCGTGCCACACCggg                                                                                                                                  <  8:106155/130‑3 (MQ=38)

                                     gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCGCCACGCCTTGCGTCGTGCCACACCggg                                                                                                                                  >  7:106155/1‑128 (MQ=38)

                                     gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGt                                                                                                                                                <  1:295646/116‑1 (MQ=255)

                                     gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGt                                                                                                                                                >  2:295646/1‑116 (MQ=255)

                                      cACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCa                                                                                                              <  1:44729/149‑1 (MQ=255)

                                       aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                             >  1:71909/1‑149 (MQ=255)

                                       aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGGGGCACGACGCAAGGCGGGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                             <  1:22758/149‑1 (MQ=255)

                                          gCCAGCCCCTTGAGGTCGTTGCGTTGGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCTCG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCggggt                                                                                                                                <  2:64573/127‑2 (MQ=21)

                                             aGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCCCCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTgg                                                                                                       >  2:250873/1‑149 (MQ=255)

                                             aGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTgg                                                                                                       <  6:170395/149‑1 (MQ=255)

                                              ggCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGc                                                                                                      <  2:111282/149‑1 (MQ=255)

                                                tccTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  2:139326/2‑149 (MQ=255)

                                                 ccTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  7:265024/1‑148 (MQ=255)

                                                 ccTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  5:358614/1‑148 (MQ=255)

                                                     gAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    <  4:128907/144‑1 (MQ=255)

                                                     gAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  3:128907/1‑144 (MQ=255)

                                                     gAGGTCGTTGCGTTTAGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                         <  3:68895/139‑1 (MQ=255)

                                                     gAGGTCGTTGCGTTTAGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                         >  4:68895/1‑139 (MQ=255)

                                                      aGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                              <  5:39466/149‑1 (MQ=255)

                                                           gTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCg                                                                                          <  2:71909/148‑1 (MQ=255)

                                                           gTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCg                                                                                          <  1:62484/148‑1 (MQ=255)

                                                                gTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                                    <  4:66302/149‑1 (MQ=255)

                                                                gTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAAGTTTGGCTCGACGTGGTCGtgtccg                                                                                    >  8:162505/1‑144 (MQ=255)

                                                                   cctacagcagGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                                    <  5:366217/142‑1 (MQ=255)

                                                                       cagcagGCGCACCTGCTCGGCCGGCCCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCCAGGCGTGGCGCCCCGCCGCGGCGTTGCTTGCCGGCGCTCAGGTGTGGCGCGACGGGGTCGGTGACGGTCAAAc                                                                             >  3:179108/1‑149 (MQ=255)

                                                                       cagcagGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAAc                                                                             >  3:46567/1‑149 (MQ=255)

                                                                          cagGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCGG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAt                                                                          <  8:205122/149‑1 (MQ=255)

                                                                           agGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAtt                                                                         <  7:293053/149‑1 (MQ=255)

                                                                              cgcCCCTGCGCGGCCGGCCCGGGGCCGGCGACTCCCTGGCCGCCCCTCCCCCCG‑GGGGGGCCCGCCGCACGGCGGGGCGCCCCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGGCGTTGACGGTAAAACCATTGTc                                                                      <  4:179108/149‑1 (MQ=255)

                                                                                cACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCAc                                                                    >  1:148113/1‑149 (MQ=255)

                                                                                   cTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACg                                                                   >  4:114992/1‑147 (MQ=255)

                                                                                     gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                                       <  2:81449/125‑1 (MQ=255)

                                                                                     gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                                       >  1:81449/1‑125 (MQ=255)

                                                                                      cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                          <  3:193052/89‑1 (MQ=255)

                                                                                      cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                          >  4:193052/1‑89 (MQ=255)

                                                                                      cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGt                                                              <  3:114992/149‑1 (MQ=255)

                                                                                      cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGt                                                              <  2:148113/149‑1 (MQ=255)

                                                                                         ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTaa                                                                                >  7:281300/1‑128 (MQ=255)

                                                                                         ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTaa                                                                                <  8:281300/128‑1 (MQ=255)

                                                                                         ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGc                                                           <  4:321605/149‑1 (MQ=255)

                                                                                           ccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGa                                                                                      <  4:58391/120‑1 (MQ=255)

                                                                                           ccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGa                                                                                      >  3:58391/1‑120 (MQ=255)

                                                                                            cggcACGGGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGa                                                        <  1:37953/149‑1 (MQ=255)

                                                                                                aCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATc                                                      >  2:77419/1‑147 (MQ=255)

                                                                                                 cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                                   <  4:139798/149‑1 (MQ=255)

                                                                                                 cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                                   <  1:77419/149‑1 (MQ=255)

                                                                                                  gTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAgg                                                  >  4:141238/1‑149 (MQ=255)

                                                                                                      ccTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                              >  8:20235/1‑149 (MQ=255)

                                                                                                       cTTCGACTAACTTGGCGCCTTTCACCCGG‑GGGTGTCACGTCGCTAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             <  5:225319/149‑1 (MQ=255)

                                                                                                       cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                              >  5:362025/1‑148 (MQ=255)

                                                                                                       cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                              <  6:362025/148‑1 (MQ=255)

                                                                                                       cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             >  2:340182/1‑149 (MQ=255)

                                                                                                       cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             <  8:265024/149‑1 (MQ=255)

                                                                                                       cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCGCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             <  6:295971/149‑1 (MQ=255)

                                                                                                          cgacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                           <  1:340182/148‑1 (MQ=255)

                                                                                                           gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGATGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgcag                                          >  1:43345/1‑148 (MQ=255)

                                                                                                           gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                                       <  8:187421/135‑1 (MQ=255)

                                                                                                           gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                                       >  7:187421/1‑135 (MQ=255)

                                                                                                                       gccgATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgg                             >  3:123904/1‑149 (MQ=255)

                                                                                                                         cgATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgggg                           >  6:38901/1‑149 (MQ=255)

                                                                                                                          gATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGt                          >  6:39968/1‑149 (MQ=255)

                                                                                                                           aTCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCt                                                          <  3:247027/116‑1 (MQ=255)

                                                                                                                           aTCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCt                                                          >  4:247027/1‑116 (MQ=255)

                                                                                                                            tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                        <  4:123904/149‑1 (MQ=255)

                                                                                                                            tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                        <  4:46567/149‑1 (MQ=255)

                                                                                                                               ccccG‑GTGTGGCACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  4:87844/1‑149 (MQ=255)

                                                                                                                                ctcG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  2:267361/3‑148 (MQ=38)

                                                                                                                                cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                              >  5:278/1‑75 (MQ=255)

                                                                                                                                cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                              <  6:278/75‑1 (MQ=255)

                                                                                                                                cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  2:108621/1‑148 (MQ=255)

                                                                                                                                cccG‑GTGTGGCACGACGCAAGGAGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  7:53034/1‑148 (MQ=255)

                                                                                                                                  cG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  8:276155/1‑146 (MQ=255)

                                                                                                                                  cG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  5:367919/1‑146 (MQ=255)

                                                                                                                                  cG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  6:306708/1‑146 (MQ=38)

                                                                                                                                      tgtgGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACg               >  4:220398/1‑149 (MQ=255)

                                                                                                                                          gCACGACGCAAGGCTTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGc                                                           <  7:242818/101‑1 (MQ=255)

                                                                                                                                          gCACGACGAAATGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGACGTAGACGGTAAAACCATTGTCACTCAGGTGGc                                                           >  8:242818/1‑101 (MQ=255)

                                                                                                                                           cACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt          <  3:87844/149‑1 (MQ=255)

                                                                                                                                           cACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt          >  3:333077/1‑149 (MQ=255)

                                                                                                                                                   aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgg                             >  3:324089/1‑122 (MQ=255)

                                                                                                                                                   aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTcgg  >  1:46744/1‑149 (MQ=255)

                                                                                                                                                   aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTcgg  >  8:234210/1‑149 (MQ=255)

CCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGCGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGG  >  NC_002947/2508759‑2509053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`