BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,508,906	A→T	5.7%	L70H (CTT→CAT) ‡	PP_2203 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,508,906	0	A	T	5.7%	282.9 / 5.5	104	L70H (CTT→CAT) ‡	PP_2203	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (50/46); new base T (4/2); total (54/50)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.87e-01

Only 100 of 109 total aligned reads displayed.

CCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGG  >  NC_002947/2508759‑2509053

cccATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAg                                                                                                                                                    <  7:179294/149‑1 (MQ=255)

 ccATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCaacg                                                                                                                                                   >  5:307329/1‑147 (MQ=255)

  cATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGc                                                                                                                                                  >  2:32286/1‑149 (MQ=255)

   aTCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCg                                                                                                                                                 >  1:233452/1‑149 (MQ=255)

        cATCCCGGGCGAGCCCATGGGCTTGCACGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCTATCACCCCG‑GTGTGGCACGACGCAAGGCGTgg                                                                                                                                              <  8:161009/147‑1 (MQ=255)

              gggCGAGCCCATGGGCATGCTCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGc                                                                                                                                      >  7:205122/1‑149 (MQ=255)

              gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGc                                                                                                                                                  <  1:209875/137‑1 (MQ=255)

              gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGc                                                                                                                                                  >  2:209875/1‑137 (MQ=255)

              gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGAGCCACGc                                                                                                                                      >  6:35341/1‑149 (MQ=255)

              gggCCACCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGGCGTTGCGGTTGGCCAGCATGC‑GCACCTGCGCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGCGTGTGGCACGACGCAAGGCGTGgcacaccg                                                                                                                                       <  1:32286/149‑7 (MQ=255)

                      ccttGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                              <  2:233452/146‑1 (MQ=255)

                      ccATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                              >  3:66302/1‑149 (MQ=255)

                      ccAGGGGCAGGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCAGGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                              <  5:99144/149‑1 (MQ=255)

                           ggattGCCCGGCACCGCCCGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACTAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCtt                                                                                                                         <  8:123958/145‑1 (MQ=255)

                             cATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGa                                                                                                                       <  1:260452/149‑1 (MQ=255)

                              ttGCCGGGCACCGCCAGGCCTTTGTGGGGGTTGGGTTTGGCCTGCAGGC‑GCACCTGCTCGGCCGGCAGGTGGCCTTCGACTAACTTGCCGCCTATCACCCCG‑GTGTGGCAGGACGCACGGCGTGGCGCAACGCGGAGGGGTTGCTTGAc                                                                                                                      <  6:290831/148‑1 (MQ=255)

                                 cccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGgc                                                                                                                   >  5:34866/1‑149 (MQ=255)

                                 cccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGgc                                                                                                                   >  2:44729/1‑149 (MQ=255)

                                     gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCGCCACGCCTTGCGTCGTGCCACACCggg                                                                                                                                  >  7:106155/1‑128 (MQ=38)

                                     gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGt                                                                                                                                                <  1:295646/116‑1 (MQ=255)

                                     gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGt                                                                                                                                                >  2:295646/1‑116 (MQ=255)

                                      cACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCa                                                                                                              <  1:44729/149‑1 (MQ=255)

                                       aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                             >  3:321605/1‑149 (MQ=255)

                                       aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                             >  1:71909/1‑149 (MQ=255)

                                       aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGGGGCACGACGCAAGGCGGGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                             <  1:22758/149‑1 (MQ=255)

                                          gCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGAAGGCGGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCTCG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCgggtt                                                                                                                                >  1:64573/1‑124 (MQ=21)

                                          gCCAGCCCCTTGAGGTCGTTGCGTTGGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCTCG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCggggt                                                                                                                                <  2:64573/127‑2 (MQ=21)

                                             aGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCCCCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTgg                                                                                                       >  2:250873/1‑149 (MQ=255)

                                             aGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTgg                                                                                                       <  6:170395/149‑1 (MQ=255)

                                              ggCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGc                                                                                                      <  2:111282/149‑1 (MQ=255)

                                                tccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  2:139326/2‑149 (MQ=255)

                                                 ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  7:265024/1‑148 (MQ=255)

                                                 ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  5:358614/1‑148 (MQ=255)

                                                 ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCg                                                                                                   <  7:63081/149‑1 (MQ=255)

                                                     gAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    >  3:128907/1‑144 (MQ=255)

                                                     gAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                    <  4:128907/144‑1 (MQ=255)

                                                     gAGGTCGTTGCGTTTAGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                         >  4:68895/1‑139 (MQ=255)

                                                     gAGGTCGTTGCGTTTAGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                         <  3:68895/139‑1 (MQ=255)

                                                      aGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                              <  5:39466/149‑1 (MQ=255)

                                                           gTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCg                                                                                          <  2:71909/148‑1 (MQ=255)

                                                                gTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                                    <  4:66302/149‑1 (MQ=255)

                                                                gTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAAGTTTGGCTCGACGTGGTCGtgtccg                                                                                    >  8:162505/1‑144 (MQ=255)

                                                                   cctacagcagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                                    <  5:366217/142‑1 (MQ=255)

                                                                       cagcagGC‑GCACCTGCTCGGCCGGCCCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCCAGGCGTGGCGCCCCGCCGCGGCGTTGCTTGCCGGCGCTCAGGTGTGGCGCGACGGGGTCGGTGACGGTCAAAc                                                                             >  3:179108/1‑149 (MQ=255)

                                                                       cagcagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAAc                                                                             >  3:46567/1‑149 (MQ=255)

                                                                          cagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCGG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAt                                                                          <  8:205122/149‑1 (MQ=255)

                                                                           agGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAtt                                                                         <  7:293053/149‑1 (MQ=255)

                                                                              c‑gcCCCTGCGCGGCCGGCCCGGGGCCGGCGACTCCCTGGCCGCCCCTCCCCCCG‑GGGGGGCCCGCCGCACGGCGGGGCGCCCCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGGCGTTGACGGTAAAACCATTGTc                                                                      <  4:179108/149‑1 (MQ=255)

                                                                                 cACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCAc                                                                    >  1:148113/1‑149 (MQ=255)

                                                                                      gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                                       >  1:81449/1‑125 (MQ=255)

                                                                                      gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                                       <  2:81449/125‑1 (MQ=255)

                                                                                       cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                          <  3:193052/89‑1 (MQ=255)

                                                                                       cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                          >  4:193052/1‑89 (MQ=255)

                                                                                       cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGt                                                              <  2:148113/149‑1 (MQ=255)

                                                                                       cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGt                                                              <  3:114992/149‑1 (MQ=255)

                                                                                          ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTaa                                                                                <  8:281300/128‑1 (MQ=255)

                                                                                          ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTaa                                                                                >  7:281300/1‑128 (MQ=255)

                                                                                          ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGc                                                           <  4:321605/149‑1 (MQ=255)

                                                                                            ccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGa                                                                                      >  3:58391/1‑120 (MQ=255)

                                                                                             cggcACGGGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGa                                                        <  1:37953/149‑1 (MQ=255)

                                                                                                 aCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATc                                                      >  2:77419/1‑147 (MQ=255)

                                                                                                  cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                                   <  1:77419/149‑1 (MQ=255)

                                                                                                  cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                                   <  4:139798/149‑1 (MQ=255)

                                                                                                   gTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAgg                                                  >  4:141238/1‑149 (MQ=255)

                                                                                                       ccTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                              >  8:20235/1‑149 (MQ=255)

                                                                                                        cTTCGACTAACTTGGCGCCTTTCACCCGG‑GGGTGTCACGTCGCTAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             <  5:225319/149‑1 (MQ=255)

                                                                                                        cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                              <  6:362025/148‑1 (MQ=255)

                                                                                                        cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                              >  5:362025/1‑148 (MQ=255)

                                                                                                        cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             <  8:265024/149‑1 (MQ=255)

                                                                                                        cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             >  2:340182/1‑149 (MQ=255)

                                                                                                        cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCGCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                             <  6:295971/149‑1 (MQ=255)

                                                                                                           cgacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                           <  1:340182/148‑1 (MQ=255)

                                                                                                            gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGATGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgcag                                          >  1:43345/1‑148 (MQ=255)

                                                                                                            gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                                       <  8:187421/135‑1 (MQ=255)

                                                                                                            gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                                       >  7:187421/1‑135 (MQ=255)

                                                                                                                        gccgATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgg                             >  3:123904/1‑149 (MQ=255)

                                                                                                                           gATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGt                          >  6:39968/1‑149 (MQ=255)

                                                                                                                            aTCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCt                                                          <  3:247027/116‑1 (MQ=255)

                                                                                                                            aTCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCt                                                          >  4:247027/1‑116 (MQ=255)

                                                                                                                             tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                        <  4:123904/149‑1 (MQ=255)

                                                                                                                             tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                        <  4:46567/149‑1 (MQ=255)

                                                                                                                               aCCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcgcg                      >  4:171906/1‑149 (MQ=255)

                                                                                                                                ccccG‑GTGTGGCACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  4:87844/1‑149 (MQ=255)

                                                                                                                                 cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                              <  6:278/75‑1 (MQ=255)

                                                                                                                                 cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                              >  5:278/1‑75 (MQ=255)

                                                                                                                                 cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                           <  6:321991/126‑1 (MQ=255)

                                                                                                                                 cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                           >  5:321991/1‑126 (MQ=255)

                                                                                                                                 cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  2:108621/1‑148 (MQ=255)

                                                                                                                                 cccG‑GTGTGGCACGACGCAAGGAGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  7:53034/1‑148 (MQ=255)

                                                                                                                                   cG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  8:276155/1‑146 (MQ=255)

                                                                                                                                   cG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                     >  6:306708/1‑146 (MQ=38)

                                                                                                                                       tgtgGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACg               >  4:220398/1‑149 (MQ=255)

                                                                                                                                           gCACGACGCAAGGCTTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGc                                                           <  7:242818/101‑1 (MQ=255)

                                                                                                                                           gCACGACGAAATGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGACGTAGACGGTAAAACCATTGTCACTCAGGTGGc                                                           >  8:242818/1‑101 (MQ=255)

                                                                                                                                            cACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt          <  3:87844/149‑1 (MQ=255)

                                                                                                                                            cACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt          >  3:333077/1‑149 (MQ=255)

                                                                                                                                                    aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgg                             >  3:324089/1‑122 (MQ=255)

                                                                                                                                                    aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTcgg  >  8:234210/1‑149 (MQ=255)

                                                                                                                                                    aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTcgg  >  1:46744/1‑149 (MQ=255)

                                                                                                                                                     aGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAgg                                                  >  5:9186/1‑100 (MQ=255)

CCCATCTCCATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGG  >  NC_002947/2508759‑2509053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`