BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,508,912	G→C	5.7%	P68R (CCA→CGA)	PP_2203 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,508,912	0	G	C	5.7%	348.1 / 7.3	105	P68R (CCA→CGA)	PP_2203	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (53/46); new base C (4/2); total (57/48)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 106 total aligned reads displayed.

CATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGCGGCC  >  NC_002947/2508767‑2509058

cATCCCGGGCGAGCCCATGGGCTTGCACGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCTATCACCCCG‑GTGTGGCACGACGCAAGGCGTgg                                                                                                                                                   <  8:161009/147‑1 (MQ=255)

      gggCGAGCCCATGGGCATGCTCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGc                                                                                                                                           >  7:205122/1‑149 (MQ=255)

      gggCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGAGCCACGc                                                                                                                                           >  6:35341/1‑149 (MQ=255)

      gggCCACCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGGCGTTGCGGTTGGCCAGCATGC‑GCACCTGCGCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCGCGTGTGGCACGACGCAAGGCGTGgcacaccg                                                                                                                                            <  1:32286/149‑7 (MQ=255)

          gAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAg                                                                                                                                       >  3:139798/1‑149 (MQ=255)

              ccttGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                                   <  2:233452/146‑1 (MQ=255)

              ccATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                                   >  3:66302/1‑149 (MQ=255)

              ccAGGGGCAGGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCAGGACGCAAGGCGTGGCGCCACGCCGAGGCGt                                                                                                                                   <  5:99144/149‑1 (MQ=255)

                   ggattGCCCGGCACCGCCCGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACTAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCtt                                                                                                                              <  8:123958/145‑1 (MQ=255)

                     cATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGa                                                                                                                            <  1:260452/149‑1 (MQ=255)

                      ttGCCGGGCACCGCCAGGCCTTTGTGGGGGTTGGGTTTGGCCTGCAGGC‑GCACCTGCTCGGCCGGCAGGTGGCCTTCGACTAACTTGCCGCCTATCACCCCG‑GTGTGGCAGGACGCACGGCGTGGCGCAACGCGGAGGGGTTGCTTGAc                                                                                                                           <  6:290831/148‑1 (MQ=255)

                         cccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGgc                                                                                                                        >  5:34866/1‑149 (MQ=255)

                         cccGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGgc                                                                                                                        >  2:44729/1‑149 (MQ=255)

                             gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCGCCACGCCTTGCGTCGTGCCACACCggg                                                                                                                                       >  7:106155/1‑128 (MQ=38)

                             gCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCGCCACGCCTTGCGTCGTGCCACACCggg                                                                                                                                       <  8:106155/130‑3 (MQ=38)

                              cACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCa                                                                                                                   <  1:44729/149‑1 (MQ=255)

                               aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                                  >  3:321605/1‑149 (MQ=255)

                               aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                                  >  1:71909/1‑149 (MQ=255)

                               aCCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGGGGCACGACGCAAGGCGGGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAt                                                                                                                  <  1:22758/149‑1 (MQ=255)

                                  gCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGAAGGCGGCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCTCG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCgggtt                                                                                                                                     >  1:64573/1‑124 (MQ=21)

                                  gCCAGCCCCTTGAGGTCGTTGCGTTGGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCTCG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCggggt                                                                                                                                     <  2:64573/127‑2 (MQ=21)

                                     aGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCCCCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTgg                                                                                                            >  2:250873/1‑149 (MQ=255)

                                      ggCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGc                                                                                                           <  2:111282/149‑1 (MQ=255)

                                        tccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                         >  2:139326/2‑149 (MQ=255)

                                         ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                         >  7:265024/1‑148 (MQ=255)

                                         ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                         >  5:358614/1‑148 (MQ=255)

                                         ccTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCg                                                                                                        <  7:63081/149‑1 (MQ=255)

                                             gAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                         >  3:128907/1‑144 (MQ=255)

                                             gAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTc                                                                                                         <  4:128907/144‑1 (MQ=255)

                                             gAGGTCGTTGCGTTTAGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                              <  3:68895/139‑1 (MQ=255)

                                             gAGGTCGTTGCGTTTAGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGttt                                                                                                              >  4:68895/1‑139 (MQ=255)

                                              aGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                                   <  5:39466/149‑1 (MQ=255)

                                                   gTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCg                                                                                               <  2:71909/148‑1 (MQ=255)

                                                   gTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCg                                                                                               <  1:62484/148‑1 (MQ=255)

                                                        gTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                                         <  4:66302/149‑1 (MQ=255)

                                                        gTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCAAGTTTGGCTCGACGTGGTCGtgtccg                                                                                         >  8:162505/1‑144 (MQ=255)

                                                           cctacagcagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACg                                                                                         <  5:366217/142‑1 (MQ=255)

                                                               cagcagGC‑GCACCTGCTCGGCCGGCCCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCCAGGCGTGGCGCCCCGCCGCGGCGTTGCTTGCCGGCGCTCAGGTGTGGCGCGACGGGGTCGGTGACGGTCAAAc                                                                                  >  3:179108/1‑149 (MQ=255)

                                                               cagcagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAAc                                                                                  >  3:46567/1‑149 (MQ=255)

                                                                  cagGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCGG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAt                                                                               <  8:205122/149‑1 (MQ=255)

                                                                   agGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCAtt                                                                              <  7:293053/149‑1 (MQ=255)

                                                                      c‑gcCCCTGCGCGGCCGGCCCGGGGCCGGCGACTCCCTGGCCGCCCCTCCCCCCG‑GGGGGGCCCGCCGCACGGCGGGGCGCCCCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGGCGTTGACGGTAAAACCATTGTc                                                                           <  4:179108/149‑1 (MQ=255)

                                                                         cACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCAc                                                                         >  1:148113/1‑149 (MQ=255)

                                                                            cTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACg                                                                        >  4:114992/1‑147 (MQ=255)

                                                                              gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                                            <  2:81449/125‑1 (MQ=255)

                                                                              gCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCTGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTg                                                                                            >  1:81449/1‑125 (MQ=255)

                                                                               cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                               <  3:193052/89‑1 (MQ=255)

                                                                               cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCt                                                                                                                               >  4:193052/1‑89 (MQ=255)

                                                                               cTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGt                                                                   <  3:114992/149‑1 (MQ=255)

                                                                                  ggccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTaa                                                                                     <  8:281300/128‑1 (MQ=255)

                                                                                    ccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGa                                                                                           <  4:58391/120‑1 (MQ=255)

                                                                                    ccggcACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGa                                                                                           >  3:58391/1‑120 (MQ=255)

                                                                                     cggcACGGGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGa                                                             <  1:37953/149‑1 (MQ=255)

                                                                                         aCGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATc                                                           >  2:77419/1‑147 (MQ=255)

                                                                                          cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                                        <  1:77419/149‑1 (MQ=255)

                                                                                          cGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAg                                                        <  4:139798/149‑1 (MQ=255)

                                                                                           gTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAgg                                                       >  4:141238/1‑149 (MQ=255)

                                                                                               ccTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                                   >  8:20235/1‑149 (MQ=255)

                                                                                                cTTCGACTAACTTGGCGCCTTTCACCCGG‑GGGTGTCACGTCGCTAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                                  <  5:225319/149‑1 (MQ=255)

                                                                                                cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                                   <  6:362025/148‑1 (MQ=255)

                                                                                                cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCtt                                                   >  5:362025/1‑148 (MQ=255)

                                                                                                cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                                  <  8:265024/149‑1 (MQ=255)

                                                                                                cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                                  >  2:340182/1‑149 (MQ=255)

                                                                                                cTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCGCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                                  <  6:295971/149‑1 (MQ=255)

                                                                                                   cgacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                                <  1:340182/148‑1 (MQ=255)

                                                                                                    gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGATGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgcag                                               >  1:43345/1‑148 (MQ=255)

                                                                                                    gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                                            <  8:187421/135‑1 (MQ=255)

                                                                                                    gacgaACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGAt                                                            >  7:187421/1‑135 (MQ=255)

                                                                                                                gccgATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgg                                  >  3:123904/1‑149 (MQ=255)

                                                                                                                  cgATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTgggg                                >  6:38901/1‑149 (MQ=255)

                                                                                                                   gATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGt                               >  6:39968/1‑149 (MQ=255)

                                                                                                                    aTCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCt                                                               >  4:247027/1‑116 (MQ=255)

                                                                                                                    aTCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCt                                                               <  3:247027/116‑1 (MQ=255)

                                                                                                                     tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                             <  4:123904/149‑1 (MQ=255)

                                                                                                                     tCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcg                             <  4:46567/149‑1 (MQ=255)

                                                                                                                       aCCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTcgcg                           >  4:171906/1‑149 (MQ=255)

                                                                                                                        ccccG‑GTGTGGCACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                          >  4:87844/1‑149 (MQ=255)

                                                                                                                         ctcG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                          >  2:267361/3‑148 (MQ=38)

                                                                                                                         cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                                   <  6:278/75‑1 (MQ=255)

                                                                                                                         cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTg                                                                                                   >  5:278/1‑75 (MQ=255)

                                                                                                                         cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                                >  5:321991/1‑126 (MQ=255)

                                                                                                                         cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTgca                                                <  6:321991/126‑1 (MQ=255)

                                                                                                                         cccG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                          >  2:108621/1‑148 (MQ=255)

                                                                                                                         cccG‑GTGTGGCACGACGCAAGGAGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                          >  7:53034/1‑148 (MQ=255)

                                                                                                                           cG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                          >  5:367919/1‑146 (MQ=255)

                                                                                                                           cG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                          >  8:276155/1‑146 (MQ=255)

                                                                                                                           cG‑GCGTGGCGCCACGCCTTGCGTCGTGCCACACCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCgg                          >  6:306708/1‑146 (MQ=38)

                                                                                                                               tgtgGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACg                    >  4:220398/1‑149 (MQ=255)

                                                                                                                                   gCACGACGCAAGGCTTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGc                                                                <  7:242818/101‑1 (MQ=255)

                                                                                                                                   gCACGACGAAATGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGACGTAGACGGTAAAACCATTGTCACTCAGGTGGc                                                                >  8:242818/1‑101 (MQ=255)

                                                                                                                                    cACGACGCAAGGCGTGGCGCCTCGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt               <  3:87844/149‑1 (MQ=255)

                                                                                                                                    cACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGAt               >  3:333077/1‑149 (MQ=255)

                                                                                                                                            tcggCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTcgg       >  6:324652/3‑149 (MQ=255)

                                                                                                                                            aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTcgg       >  1:46744/1‑149 (MQ=255)

                                                                                                                                            aaGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTcgg       >  8:234210/1‑149 (MQ=255)

                                                                                                                                             aGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAgg                                                       >  5:9186/1‑100 (MQ=255)

                                                                                                                                                cGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                                  >  7:23976/1‑102 (MQ=255)

                                                                                                                                                cGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTg                                                  <  8:23976/102‑1 (MQ=255)

                                                                                                                                                cGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGGGGTCGGTGACGGTAAAACCATTGGCACGCAGGTGGCCGATCCAGGCCTGGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCggcggc   <  6:358614/149‑1 (MQ=255)

                                                                                                                                                 gTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGCGGcc  >  4:58497/1‑149 (MQ=255)

CATGCCGGGCGAGCCCATGGGCATGCCCGGCACCGCCAGGCCCTTGAGGTCGTTGCGTTTGGCCAGCAGGC‑GCACCTGCTCGGCCGGCACGTGGCCTTCGACGAACTTGCCGCCGATCACCCCG‑GTGTGGCACGACGCAAGGCGTGGCGCCACGCCGAGGCGTTGCTTGACGGCGCTCATGTTTGGCTCGACGTGGTCGTTGACGGTAAAACCATTGTCACGCAGGTGGCTGATCCAGGCCTTGCAGCAGCCGCAGTTGGGGTCGCGGTAGACGTCGATGGTCTCGGCGGCC  >  NC_002947/2508767‑2509058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`