Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,618,706 A→T 6.9% Y203N (TAC→AAC)  PP_2292 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,618,7060AT 6.9% 166.5 / 3.3 58Y203N (TAC→AAC) PP_2292hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (30/24);  new base T (2/2);  total (32/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

ATTTGTTTCTAGCCTCGCTTTCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATCGATTCCAC  >  NC_002947/2618568‑2618853
                                                                                                                                          |                                                                                                                                                    
aTTTGTTTCTAGCCTCGCTTTCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAgc                                                                                                                                            >  8:4409/1‑149 (MQ=255)
      ttCTAGCCTCGCTTTCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTg                                                                                                                                      >  5:251567/1‑149 (MQ=255)
           gCCTCGCTTTCGTTTTTGAACGTTAAGTAACACATCGCAAGCGCTTTGCTGACTATTTACTCTCGCGGAGGAAATGGCTGGAATGTATAAATGAGTCACAATATATCGACTGCGGCCAATTTCTTGTAGACGTCCTGCGCACTGCCGa                                                                                                                                  >  4:179834/1‑148 (MQ=255)
            ccTCGCTTTCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAAt                                                                                                                                >  3:265396/1‑149 (MQ=255)
              tCGCTTTCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAAtt                                                                                                                               >  3:232765/1‑148 (MQ=255)
                  tttCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCa                                                                                                                                         <  1:24813/134‑1 (MQ=255)
                  tttCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCa                                                                                                                                         >  2:24813/1‑134 (MQ=255)
                   ttCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTTGAAGTCCAGCGCACTGCCGAATTTAgtgt                                                                                                                         >  5:10799/1‑149 (MQ=255)
                   ttCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTg                                                                                                                                      >  7:203394/1‑136 (MQ=255)
                   ttCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTg                                                                                                                                      <  8:203394/136‑1 (MQ=255)
                    tCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAgtg                                                                                                                          <  6:251567/147‑1 (MQ=255)
                      gTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTTGAAGTCCAGCGCACTGCCGAATTTAGTGTCAg                                                                                                                      >  5:86455/1‑149 (MQ=255)
                                gTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑cc                                                                                                           <  6:126247/149‑1 (MQ=255)
                                   aaGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCAt                                                                                                         <  7:4409/148‑1 (MQ=255)
                                            aTCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGc                                                                                                >  6:55551/1‑148 (MQ=255)
                                                cAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTg                                                                                           <  6:28765/149‑1 (MQ=255)
                                                     gcTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCAt                                                                                                         <  3:138093/130‑1 (MQ=255)
                                                     gcTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCAt                                                                                                         >  4:138093/1‑130 (MQ=255)
                                                       tttGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAg                                                                                    >  2:229008/1‑149 (MQ=255)
                                                           ctctctATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGtgc                                                                                 <  4:232765/148‑1 (MQ=255)
                                                           ctatctATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGCGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGtgct                                                                                <  4:7147/146‑1 (MQ=255)
                                                                tATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGt                                                                           >  7:297336/1‑149 (MQ=255)
                                                                tATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGt                                                                           >  5:101312/1‑149 (MQ=255)
                                                                tATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGt                                                                           >  2:41346/1‑149 (MQ=255)
                                                                  ttGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGATTGCTGCCAATGTATTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CAATGAGTTACGCCATTGCGATTAGTGCTTCCGTTg                                                                         >  1:94018/1‑149 (MQ=255)
                                                                    gACTCTCGCGGATGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTATAACTCCAGCGCACTGGCGAATTTAGTGTCAGTAAAATCGCCCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTg                                                                         <  1:229008/148‑1 (MQ=255)
                                                                     aCTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTc                                                                      <  5:55551/149‑1 (MQ=255)
                                                                       tctcGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTcc                                                                     >  4:188799/1‑147 (MQ=255)
                                                                           gcgGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTTGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACatat                                                                <  6:86455/149‑1 (MQ=255)
                                                                           gcgGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACata                                                                 <  1:41346/148‑1 (MQ=255)
                                                                             ggaggaAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATAttt                                                              <  8:297336/149‑1 (MQ=255)
                                                                                ggaAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTTGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTc                                                           <  6:10799/149‑1 (MQ=255)
                                                                                  aaaTGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTc                                                           >  5:267134/1‑147 (MQ=255)
                                                                                  aaaTGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTc                                                           <  6:267134/147‑1 (MQ=255)
                                                                                        cTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGc                                                   <  4:265396/149‑1 (MQ=255)
                                                                                                tAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCtata                                               <  4:209414/145‑1 (MQ=255)
                                                                                                tAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCAGTTGGTCACATATTTGTCGGAGTCGCtata                                               >  3:209414/1‑145 (MQ=255)
                                                                                                  gAAATGAGTCACAATATATCGACTGCTGCCAATGTCATGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAAttt                                         <  2:94018/149‑1 (MQ=255)
                                                                                                               atatatCGTCTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGATTTACGCCATTTCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAAtttt                            <  3:179834/149‑1 (MQ=255)
                                                                                                                tatatCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAAttttt                           <  8:169324/149‑1 (MQ=255)
                                                                                                                tatatCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAAttttt                           >  7:169324/1‑149 (MQ=255)
                                                                                                                    tCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTgg                         >  3:175801/1‑147 (MQ=255)
                                                                                                                    cgatctgctgCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTgga                        <  3:38364/144‑1 (MQ=255)
                                                                                                                           ctgcCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTgga                        >  4:38364/1‑141 (MQ=255)
                                                                                                                           ctgcCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACgc                <  2:339369/149‑1 (MQ=255)
                                                                                                                            tgcCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCg                                                          >  4:112633/1‑106 (MQ=255)
                                                                                                                            tgcCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCg                                                          <  3:112633/106‑1 (MQ=255)
                                                                                                                            tgcCAAGGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑GCATGAGTTACGCCATTGCGATTAGTGCGGCCGTTGGTCACATATTAGGCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTggaaggaagg                 >  6:255324/1‑145 (MQ=255)
                                                                                                                              ccAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACgcagc             <  4:175801/149‑1 (MQ=255)
                                                                                                                              ccAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACgcagc             <  7:203817/149‑1 (MQ=255)
                                                                                                                               cAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACgcagca            >  1:3319/1‑149 (MQ=255)
                                                                                                                               aaaTGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACgcagca            >  1:3322/2‑149 (MQ=255)
                                                                                                                                 aTGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATc          >  2:84421/1‑149 (MQ=255)
                                                                                                                                     cTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATCGAtt      >  3:293904/1‑149 (MQ=255)
                                                                                                                                      ttGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATCGATTc     >  3:206805/1‑149 (MQ=255)
                                                                                                                                        gTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATCGATTcc    >  8:90454/1‑148 (MQ=255)
                                                                                                                                         tAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATCGATTCCAc  >  7:229501/1‑149 (MQ=255)
                                                                                                                                         tAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATCGATTCCAc  >  2:281826/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
ATTTGTTTCTAGCCTCGCTTTCGTTTTTGCACGTTAAGTAACACATCGCAAGCGCTTTGCTCTCTATTGACTCTCGCGGAGGAAATGGCTGGAATGTAGAAATGAGGCACAATATATCGACTGCTGCCAATGTCTTGTAGAAGTCCAGCGCACTGCCGAATTTAGTGTCAGTAAAATCG‑CCATGAGTTACGCCATTGCGATTAGTGCTGCCGTTGGTCACATATTTGTCGGAGTCGCTATAGAATTTTTGGAGGAATTTTTGGAAGGAACGCAGCATCGATTCCAC  >  NC_002947/2618568‑2618853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑