Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,339	T→A	7.1%	Q15L (CAG→CTG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,339	0	T	A	7.1%	207.4 / 5.2	70	Q15L (CAG→CTG)	trmE	GTPase
Reads supporting (aligned to +/- strand):  ref base T (39/26);  new base A (2/3);  total (41/29)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.45e-01

CTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTT  >  NC_002947/6196‑6485                                                                                                                                                 |                                                                                                                                                   cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGcc                                                                                                                                               >  8:3786/1‑149 (MQ=255)            aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                                    <  6:148016/149‑3 (MQ=255)             ccAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtgg                                                                                                                                   <  8:182581/149‑4 (MQ=255)              cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                                  <  7:329004/149‑1 (MQ=255)              cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                                  >  4:301441/1‑149 (MQ=255)              cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGGCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGCGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                                  >  8:58336/1‑149 (MQ=255)                   cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGAt                                                                                                                              <  1:89040/149‑1 (MQ=255)                       aTCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGtt                                                                                                                          >  6:67205/1‑148 (MQ=255)                       aTCGTCGCGGAATGGACCGTAATGGGAATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCTGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGtt                                                                                                                          >  8:155248/1‑148 (MQ=255)                        tcgtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTc                                                                                                                        <  7:3786/149‑1 (MQ=255)                                      aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc                                                                                                           <  8:143173/148‑1 (MQ=255)                                      aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCa                                                                                                          >  4:352612/1‑149 (MQ=255)                                          tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGAGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATgat                                                                                                      >  6:360836/1‑147 (MQ=255)                                              gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                                    >  5:334034/1‑113 (MQ=38)                                              gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                                    <  6:334034/115‑3 (MQ=38)                                               ggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                 <  3:352612/149‑1 (MQ=255)                                                  aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                              >  7:300213/1‑149 (MQ=255)                                                  aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                              >  8:86748/1‑149 (MQ=255)                                                     ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                     <  6:327852/139‑1 (MQ=255)                                                     ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                     >  5:327852/1‑139 (MQ=255)                                                      cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGGCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGGCGTGGCGATTGCGGCGATGGTTTCACGGACAGTGGTCATGCTCGAAAGCCTCTa                                                                                          <  5:360836/149‑1 (MQ=255)                                                       gcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                         >  4:334401/1‑149 (MQ=255)                                                             tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaa                                                                                    >  5:345328/1‑148 (MQ=255)                                                                 ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                                >  8:34920/1‑148 (MQ=255)                                                                   tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                             <  7:86748/149‑1 (MQ=255)                                                                       gCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                         >  7:311462/1‑149 (MQ=255)                                                                       gCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                         <  7:155248/149‑1 (MQ=255)                                                                         cGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                          >  3:131079/1‑146 (MQ=255)                                                                            tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCa                                                                     >  5:205462/1‑148 (MQ=255)                                                                                 aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                                <  3:334401/148‑1 (MQ=255)                                                                                  aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                              >  2:334053/1‑149 (MQ=255)                                                                                      gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                          >  6:224936/1‑149 (MQ=255)                                                                                      gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                          >  5:81461/1‑149 (MQ=255)                                                                                      gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                          >  2:131220/1‑149 (MQ=255)                                                                                      gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCAAGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                          >  8:277016/1‑149 (MQ=255)                                                                                           ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                 >  7:64888/1‑105 (MQ=255)                                                                                           ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                 <  8:64888/105‑1 (MQ=255)                                                                                           ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAgg                                                     <  5:67205/149‑1 (MQ=255)                                                                                               ggCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAgggg                                                   <  6:345328/147‑1 (MQ=255)                                                                                                 cAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                       <  1:62537/109‑1 (MQ=255)                                                                                                 cAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                       >  2:62537/1‑109 (MQ=255)                                                                                                   gCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGttt                                             <  4:310833/149‑1 (MQ=255)                                                                                                          ggCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTc                                      <  6:205462/149‑1 (MQ=255)                                                                                                                     ccTGACAATTCCCACACCGCCCCGGCCCTGGGCAGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTAATGCTCGAAAGCCTCTACGACAAAAACGACAGAAAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTAACAGGATAGc                            >  2:71735/1‑149 (MQ=255)                                                                                                                      cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                            <  4:227367/68‑1 (MQ=255)                                                                                                                      cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                            >  3:227367/1‑68 (MQ=255)                                                                                                                      cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCg                           >  1:45881/1‑149 (MQ=255)                                                                                                                        gACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGAGGTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACAAGGGGGCGTTTATTATTCACAGGCTAGCGTa                         >  4:237769/1‑149 (MQ=255)                                                                                                                         aCAATTCCCACACCGCCCCGGCCCTGGGCGGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTa                         <  2:283139/148‑1 (MQ=255)                                                                                                                          cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAtt                       <  5:224936/149‑1 (MQ=255)                                                                                                                          cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAtt                       <  2:76934/149‑1 (MQ=255)                                                                                                                              tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTcaca                                   >  2:292739/1‑133 (MQ=255)                                                                                                                              tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGCCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTcaca                                   <  1:292739/133‑1 (MQ=255)                                                                                                                                ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg                 >  6:258032/1‑149 (MQ=255)                                                                                                                                ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg                 >  6:107083/1‑149 (MQ=255)                                                                                                                                 cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                            >  2:57929/1‑121 (MQ=255)                                                                                                                                 cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                            <  1:57929/121‑1 (MQ=255)                                                                                                                                 cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCCCAGGCTAGCGTATTAGCCCGt                >  8:67483/1‑149 (MQ=255)                                                                                                                                  ccatcGCCACGGCCCAGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt                >  7:90100/5‑148 (MQ=255)                                                                                                                                  acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAACAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc               <  3:301441/149‑1 (MQ=255)                                                                                                                                  acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCCCTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt                >  3:222117/1‑148 (MQ=255)                                                                                                                                    acCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCaa             <  2:150373/149‑1 (MQ=255)                                                                                                                                      cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag            >  4:59370/1‑148 (MQ=255)                                                                                                                                        ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag         >  2:52805/1‑149 (MQ=255)                                                                                                                                        ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag         >  5:234092/1‑149 (MQ=255)                                                                                                                                         cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag         >  2:186182/1‑148 (MQ=255)                                                                                                                                         cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag         <  1:186182/148‑1 (MQ=255)                                                                                                                                           cGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagcag      >  8:208680/1‑149 (MQ=255)                                                                                                                                           cGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagcag      >  4:32975/1‑149 (MQ=255)                                                                                                                                               ccTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCttt  <  1:52805/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   CTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTT  >  NC_002947/6196‑6485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑