Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,715,414 C→T 9.2% C43C (TGC→TGT)  cti → esterified fatty acid cis/trans isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,715,4140CT 9.2% 117.1 / 2.3 44C43C (TGC→TGT) ctiesterified fatty acid cis/trans isomerase
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base T (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.23e-01

AGAACCAGGACTTTCCGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGCACACA  >  NC_002947/2715266‑2715562
                                                                                                                                                     |                                                                                                                                                    
aGAACCAGGACTTTCCGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGc                                                                                                                                                      >  1:205436/1‑149 (MQ=255)
     cAGGACTTTCCGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGc                                                                                                                                                 <  5:93609/149‑1 (MQ=255)
               cGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGc                                                                                                                                        <  6:353442/148‑1 (MQ=255)
                    aTGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCaa                                                                                                                                  <  6:149417/149‑1 (MQ=255)
                     tGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTg                                                                                                                                     >  8:216422/1‑145 (MQ=255)
                     tGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTg                                                                                                                                     <  7:216422/145‑1 (MQ=255)
                                          gcgcCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAg                                                                                                            >  8:331834/1‑149 (MQ=255)
                                                  gCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGaaa                                                                                                     <  7:97896/148‑1 (MQ=255)
                                                      tGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                  >  3:21790/1‑147 (MQ=255)
                                                      tGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                  <  4:21790/147‑1 (MQ=255)
                                                      tGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCt                                                                                                >  7:5250/1‑149 (MQ=255)
                                                         tcatcaGCGGCGCGGTATTCGGGCAGGCCCCCCAGGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTg                                                                                               >  1:48637/1‑148 (MQ=255)
                                                          catcaGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCC‑GGACATTCAACCGATCTTCACCGAGAAGTGCGTGACCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAgg                                                                                           >  3:343226/1‑149 (MQ=255)
                                                           atcaGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTTGAGCTggcaggcg                                                                                                  >  4:226366/1‑136 (MQ=255)
                                                           atcaGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTTGAGCTggcaggcg                                                                                                  <  3:226366/142‑7 (MQ=255)
                                                               gcggcgCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAAGCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCCAGCTGGAAAGCCCTGAAGGcgcg                                                                                       >  6:119536/1‑149 (MQ=255)
                                                                    gcgGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTAcg                                                                                  >  7:147355/1‑149 (MQ=255)
                                                                       gTATTCGGGCAGGCCCACCA‑GTCGAGCCCGGCTATTACCAACACCCGGGAAATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCAACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTCGAAAGCCCTGAAGGCGCGGTACGCgg                                                                               >  4:100836/1‑149 (MQ=255)
                                                                          ttCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGACGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCgggg                                                                             <  5:119013/148‑1 (MQ=255)
                                                                             gggCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACACcgggacct                                                                         >  6:171956/1‑145 (MQ=255)
                                                                                     cccccA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGTGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAg                                                                                                            <  6:352390/106‑1 (MQ=255)
                                                                                     cccccA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGTGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAg                                                                                                            >  5:352390/1‑106 (MQ=255)
                                                                                      ccccA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTccc                                                                >  8:141678/1‑149 (MQ=255)
                                                                                        ccA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCgg                                                              >  8:118807/1‑149 (MQ=255)
                                                                                        ccA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCgg                                                              >  7:189281/1‑149 (MQ=255)
                                                                                         cA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGgt                                                             <  8:147355/149‑1 (MQ=255)
                                                                                               gAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTAcc                                                        <  7:141678/149‑1 (MQ=255)
                                                                                                   ccGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAggg                                                    >  2:265520/1‑149 (MQ=255)
                                                                                                    cGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCgggg                                                                             <  5:69314/123‑1 (MQ=255)
                                                                                                    cGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGAGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCgggg                                                                             >  6:69314/1‑123 (MQ=255)
                                                                                                          tttCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCgg                                             <  2:48637/149‑1 (MQ=255)
                                                                                                          tttCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCg                                              <  8:189281/148‑1 (MQ=255)
                                                                                                             ccTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCgat                                            >  8:5541/1‑145 (MQ=255)
                                                                                                                acacCCGGGACATTCAACCGATCTTCACCGAGAAGTGTGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAg                                       >  6:154073/1‑149 (MQ=255)
                                                                                                                 cacCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAgg                                      <  7:118807/149‑1 (MQ=255)
                                                                                                                   cccGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCa                                    <  2:205436/149‑1 (MQ=255)
                                                                                                                     cGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAgg                                      <  2:84454/145‑1 (MQ=255)
                                                                                                                     cGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAgg                                      >  1:84454/1‑145 (MQ=255)
                                                                                                                     cGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGt                                  <  7:331834/149‑1 (MQ=255)
                                                                                                                           aTTCAACCGATCTTCACCGAGAAGTGTGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCccac                            <  5:154073/149‑1 (MQ=255)
                                                                                                                            ttCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCccac                            >  5:68656/1‑148 (MQ=255)
                                                                                                                             tCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCccacca                          >  8:40861/1‑149 (MQ=255)
                                                                                                                                aCCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCAcgc                       <  6:68656/149‑1 (MQ=255)
                                                                                                                                             gagaAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCa                                                       <  7:267784/104‑1 (MQ=255)
                                                                                                                                             gagaAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCa                                                       >  8:267784/1‑104 (MQ=255)
                                                                                                                                                     cGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGcacaca  <  8:5250/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGAACCAGGACTTTCCGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCA‑GTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGAAGGCGCGGTACGCGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGCACACA  >  NC_002947/2715266‑2715562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑