Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,722,218 T→C 8.5% S247P (TCG→CCG)  PP_2382 → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,722,2180TC 8.5% 162.4 / 5.0 59S247P (TCG→CCG) PP_2382putative transporter
Reads supporting (aligned to +/- strand):  ref base T (24/30);  new base C (3/2);  total (27/32)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

GCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGTTACTGGATGCT  >  NC_002947/2722071‑2722360
                                                                                                                                                   |                                                                                                                                              
gCATTCGACGGGTGCGCCATAATCAGCGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATc                                                                                                                                               <  6:279220/149‑1 (MQ=255)
        tGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGATTTATCCTTCCAGCATCGGTGCTgg                                                                                                                                       <  8:169991/149‑1 (MQ=255)
          ggTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGa                                                                                                                                      <  7:330712/148‑1 (MQ=255)
                 cATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCg                                                                                                                              >  3:42957/1‑149 (MQ=255)
                      tCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTTGCTTTATCCTTCCAGCATCGGTGc                                                                                                                                          >  8:210377/1‑132 (MQ=255)
                      tCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTTGCTTTATCCTTCCAGCATCGGTGc                                                                                                                                          <  7:210377/132‑1 (MQ=255)
                               cggcggGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAgcggc                                                                                                                 <  7:116763/148‑1 (MQ=255)
                                 gcggGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGt                                                                                                              >  2:278599/1‑149 (MQ=255)
                                         tgtgAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACg                                                                                                      >  7:216916/1‑149 (MQ=255)
                                          gtgGGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACgt                                                                                                     <  7:70413/149‑1 (MQ=255)
                                             aGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGtt                                                                                                  >  1:280438/1‑149 (MQ=255)
                                                   ttGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACt                                                                                            <  1:46247/149‑1 (MQ=255)
                                                     gAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTg                                                                                           <  1:278599/148‑1 (MQ=255)
                                                        ggCCGGCAATGCGCCGATGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTg                                                                                       <  8:128248/149‑1 (MQ=255)
                                                          ccGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCg                                                                                     >  1:271264/1‑149 (MQ=255)
                                                             gCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCgg                                                                                    >  4:217157/1‑147 (MQ=255)
                                                                    gcCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACgg                                                                           >  3:21945/1‑149 (MQ=255)
                                                                    gcCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCCCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACgg                                                                           <  7:189066/149‑1 (MQ=255)
                                                                      cGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACggtg                                                                         <  4:5184/149‑1 (MQ=255)
                                                                         tgttCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGttt                                                                                                 <  4:41847/122‑1 (MQ=255)
                                                                         tgttCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGttt                                                                                                 >  3:41847/1‑122 (MQ=255)
                                                                             cGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTgggg                                                                   <  4:42957/148‑1 (MQ=255)
                                                                              gCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTggggg                                                                  <  8:141550/148‑1 (MQ=255)
                                                                                  tCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATg                                                             <  2:271264/149‑1 (MQ=255)
                                                                                    aTTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGct                                                           >  4:50338/1‑149 (MQ=255)
                                                                                    aTTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGct                                                           >  4:258768/1‑149 (MQ=255)
                                                                                        gCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTggggg                                                                  <  2:345134/138‑1 (MQ=255)
                                                                                        gCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTggggg                                                                  >  1:345134/1‑138 (MQ=255)
                                                                                          tGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTacac                                                     >  1:275288/1‑149 (MQ=255)
                                                                                               cacaCTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCt                                                >  8:119458/1‑149 (MQ=255)
                                                                                                acacTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCtt                                               <  8:216916/149‑1 (MQ=255)
                                                                                                  acTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTg                                                                                                            <  4:274677/86‑1 (MQ=255)
                                                                                                  acTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTg                                                                                                            >  3:274677/1‑86 (MQ=255)
                                                                                                      tgtgCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGctc                                                          >  3:84323/1‑132 (MQ=255)
                                                                                                      tgtgCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGctc                                                          <  4:84323/132‑1 (MQ=255)
                                                                                                      tgtgCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATcc                                         >  6:144991/1‑149 (MQ=255)
                                                                                                        tgCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCcgc                                       <  2:280438/149‑1 (MQ=255)
                                                                                                        tgCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCcg                                        <  1:342611/148‑1 (MQ=255)
                                                                                                          cGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGAt                                                                                                         <  4:234466/81‑1 (MQ=255)
                                                                                                          cGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGAt                                                                                                         >  3:234466/1‑81 (MQ=255)
                                                                                                               ttCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACaa                                 <  2:275288/148‑1 (MQ=255)
                                                                                                                tCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAAc                                <  7:119458/148‑1 (MQ=255)
                                                                                                                    cACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAg                           >  3:51352/1‑149 (MQ=255)
                                                                                                                     aCGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAg                           >  3:26457/1‑148 (MQ=255)
                                                                                                                         cGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACTCGCGCTTCAATCAGCGCACTACCGCAGGAttt                      >  6:251063/1‑148 (MQ=255)
                                                                                                                            tGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGtg                   <  3:258768/149‑1 (MQ=255)
                                                                                                                                  gCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGt             <  3:217157/149‑1 (MQ=255)
                                                                                                                                   cTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGctc                                                          <  3:291856/103‑1 (MQ=255)
                                                                                                                                   cTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGctc                                                          >  4:291856/1‑103 (MQ=255)
                                                                                                                                       aTCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGTAGGATTGGTGCTGCGTTACTg        >  7:138238/1‑149 (MQ=255)
                                                                                                                                       aTCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGTTACt         <  4:51352/148‑1 (MQ=255)
                                                                                                                                        tCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGTTACTg        <  3:50338/148‑1 (MQ=255)
                                                                                                                                          cTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGTTACTGGAt     >  3:93455/1‑149 (MQ=255)
                                                                                                                                           ctCCAGCACCGATGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTgg                     <  1:253851/131‑1 (MQ=255)
                                                                                                                                           ctCCAGCACCGATGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTgg                     <  1:248763/131‑1 (MQ=255)
                                                                                                                                           ctCCAGCACCGATGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTgg                     >  2:248763/2‑132 (MQ=255)
                                                                                                                                           ctCCAGCACCGATGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTgg                     >  2:253851/2‑132 (MQ=255)
                                                                                                                                           ctCCAGCACCGATGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGTTACTGGATg    >  7:274492/2‑149 (MQ=255)
                                                                                                                                              cAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGTTACTGGATGct  <  4:26457/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
GCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTCCAGCATCGGTGCTGGAGCACTTCGCCTCTGAAGCGGCGGTTGGATACGTGTTTTCACTGCTTGCGGCAGGCACGGTGGTGGGGGGCATGCTCTACACGCGCTTCAATCCGCGCACAACCGCAGGATTGGTGCTGCGTTACTGGATGCT  >  NC_002947/2722071‑2722360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑