Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,777,007 C→G 10.0% W166C (TGG→TGC)  PP_2429 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,777,0070CG10.0% 137.5 / 7.5 50W166C (TGG→TGC) PP_2429membrane protein
Reads supporting (aligned to +/- strand):  ref base C (22/23);  new base G (3/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

CGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTG  >  NC_002947/2776860‑2777151
                                                                                                                                                   |                                                                                                                                                  
cgcgAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGc                                                                                                                                                    <  4:57115/148‑1 (MQ=255)
        gTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGGCTGCAGGCACC‑ggggc                                                                                                                                          <  5:317356/149‑3 (MQ=255)
            acacGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTcc                                                                                                                                      >  4:190258/1‑149 (MQ=255)
            acacGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTc                                                                                                                                       <  7:311875/148‑1 (MQ=255)
              acGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATATTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTcccc                                                                                                                                    <  6:281881/149‑1 (MQ=255)
                    aGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAccccc                                                                                                                              >  6:266323/1‑149 (MQ=255)
                     gCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACACCCGCCAACACTTGGTTGAAAACCCGCAACCGCCCCCGTGCCTGCAGCCACC‑GGGCGCTCACCAccccc                                                                                                                              >  4:149756/1‑148 (MQ=255)
                                tCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCACCACCGCCCAg                                                                                                                  >  8:162802/1‑149 (MQ=255)
                                    gCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAggcgg                                                                                                              <  1:185344/149‑1 (MQ=255)
                                             cGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACAcc                                                                                                                        <  3:165470/130‑1 (MQ=255)
                                             cGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACATGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACAcc                                                                                                                        >  4:165470/1‑130 (MQ=255)
                                              ggTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCgg                                                                                                    <  3:149756/149‑1 (MQ=255)
                                                tCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACCCCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCGCCCCACCCCCAACACCGCCCCGGCGGCCCTGCTCgg                                                                                                    >  6:332349/1‑147 (MQ=255)
                                                    gTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCGGCAGCCCCC‑GGGGGCGGCCCCCCCCCAACACCGCCCAGGCGGCCCAGCGCGGCCGAGc                                                                                              >  8:52741/1‑149 (MQ=255)
                                                         ggCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCa                                                                                         <  5:266323/149‑1 (MQ=255)
                                                             cAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCG‑CCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAg                                                                                    <  7:101150/149‑1 (MQ=255)
                                                                gCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACCCCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGaa                                                                                  >  4:288299/1‑149 (MQ=255)
                                                                  cAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCCGTGCCTGCAGCCACC‑CGGCGCTCCCCCCCCCCAACACCCCCCAGGCGGCCAAGCACGGCAGAGCGAACAGCAAGAa‑cc                                                                               >  2:4408/1‑148 (MQ=255)
                                                                  cAATACTGCAGCCCATGACCATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGCAGCCACCACCCACC‑CGGCGCTCCCCACCCCCAACACCCCCCAGGCCGCCATGCTCGGCAGAGCGATCAGCAacaa‑ca                                                                               >  4:206109/1‑149 (MQ=255)
                                                                       cTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAc                                                                                                                                  <  4:279726/94‑1 (MQ=255)
                                                                       cTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAc                                                                                                                                  >  3:279726/1‑94 (MQ=255)
                                                                       cTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAgc                                                                          <  5:332349/149‑1 (MQ=255)
                                                                             cccATGCCGATATCAACAAACCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCAGCAGCCACC‑GGGCGCGCCCCACCCCCAACCCCGCCCAGGCCGCCATGCTCGGCAGAGCGATCCGCCAGAA‑CCCCCCCGCCAGc                                                                    >  8:249465/1‑149 (MQ=255)
                                                                                aTGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCaa                                                                                     <  5:309608/130‑1 (MQ=255)
                                                                                    cGATATCAACAAAACCCCCTCCAACACTTGGTTTAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCt                                                                                                       <  1:340510/108‑1 (MQ=255)
                                                                                        atCAACTATACCCCCGCCAACTCTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACt                                                           <  7:166960/147‑1 (MQ=255)
                                                                                           aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCa                                                       <  1:122110/148‑1 (MQ=255)
                                                                                            acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCagag                                                                                               <  7:92296/108‑1 (MQ=255)
                                                                                            acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACCACCAGCGCCAGCTGCCACACTGGCaa                                                      <  1:4408/149‑1 (MQ=255)
                                                                                            acaaAACCCCCGCCAACACATGGGTGAACACACCCAACCGCACCGCTGCCTGCCGCCCCC‑GGGCGCTCCCCACCCCCCACACCCCCCAGGCGCGCATGCTCGGAAGACCGAAAAGCAAGAA‑AACCAGCGCCAGCTGCCACACAGGCa                                                       >  2:105916/1‑147 (MQ=255)
                                                                                             caaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCAGTGCCTGCAGCCACC‑CGGCGCTCCCCCCCCCCAACACCCCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCCCCCGCTGCCACACAGGCAacg                                                    >  4:150395/1‑149 (MQ=255)
                                                                                                aaCCCCCGCCAACACTTGGATGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAacgacg                                                 <  3:206109/149‑1 (MQ=255)
                                                                                                    cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAgc                                                                          >  4:346755/1‑120 (MQ=255)
                                                                                                    cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAgc                                                                          <  3:346755/120‑1 (MQ=255)
                                                                                                     ccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGcgcg                                            <  7:162802/149‑1 (MQ=255)
                                                                                                         cAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCaa                                        <  3:190258/149‑1 (MQ=255)
                                                                                                             acTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACCGCGCGGCAAATAc                                    >  2:294876/1‑149 (MQ=255)
                                                                                                               ttGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAccc                                  >  5:83454/1‑149 (MQ=255)
                                                                                                                          cccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGcc                       <  7:52741/149‑1 (MQ=255)
                                                                                                                           ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGcc                                                                                                            <  5:62966/64‑1 (MQ=255)
                                                                                                                           ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGcc                                                                                                            >  6:62966/1‑64 (MQ=255)
                                                                                                                           ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCa                      >  3:177424/1‑149 (MQ=255)
                                                                                                                              cAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACgg                   <  4:177424/149‑1 (MQ=255)
                                                                                                                                gagcgcccGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGa                               <  4:74350/131‑1 (MQ=255)
                                                                                                                                gagcgcccGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGa                               >  3:74350/6‑136 (MQ=255)
                                                                                                                                   gCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATc              <  3:288299/149‑1 (MQ=255)
                                                                                                                                     cccGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATc              >  3:47502/1‑148 (MQ=255)
                                                                                                                                      ccGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAc           >  1:132276/1‑149 (MQ=255)
                                                                                                                                             cTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCt    >  3:55179/1‑149 (MQ=255)
                                                                                                                                              tGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGCCGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCtt   >  2:223524/1‑149 (MQ=255)
                                                                                                                                               gCAGGCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTg  >  6:168210/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAA‑CACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACCAGACCTTG  >  NC_002947/2776860‑2777151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑