| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,796,585 | T→C | 12.5% | intergenic (+18/‑155) | PP_t42 → / → PP_t43 | tRNA‑Val/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,796,585 | 0 | T | C | 12.5% | 117.8 / 8.6 | 48 | intergenic (+18/‑155) | PP_t42/PP_t43 | tRNA‑Val/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (18/24); new base C (4/2); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
AAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCA > NC_002947/2796443‑2796730 | aaaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGaaa < 4:28104/149‑1 (MQ=255)aaaGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGaaa < 5:238176/149‑1 (MQ=255) tGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCa < 8:1356/148‑1 (MQ=255) gTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAg < 5:148820/123‑1 (MQ=255) gTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAg > 6:148820/1‑123 (MQ=255) gTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGACCCTTTCGGATCACCCCttttt < 1:173169/137‑1 (MQ=14) gTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGACCCTTTCGGATCACCCCttttt > 2:173169/1‑137 (MQ=14) tAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGt < 8:301587/125‑1 (MQ=255) tAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGt > 7:301587/1‑125 (MQ=255) gAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGcc > 2:88044/1‑149 (MQ=255) cacaCGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCgg < 8:57033/149‑1 (MQ=255) cacGACATTCACTTAGCTCAGTAGGTTAGAGCACCACCTTGACATGGTGGGGGTCTTTGGTTCTATTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGa < 8:110659/149‑1 (MQ=21) acGTCAGGCAGATAGGTCTGTTGGTTAGAGCATCACGTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTATCTATTCGTAATAGGTTTGTTCCGAAAGGGTCAGCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGaa < 4:106328/149‑1 (MQ=18) gACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATa < 5:294898/149‑1 (MQ=255) gCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGCGCGGGAATACcgct > 8:108525/1‑148 (MQ=255) acaTAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCgg > 4:155562/1‑148 (MQ=255) gCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGa < 8:95506/149‑1 (MQ=255) cTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGt < 1:58027/113‑1 (MQ=255) cTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGt > 2:58027/1‑113 (MQ=255) gTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCG‑AAAAGGGGTGATCCGAAAGGGTCACCCCttttt < 7:256821/101‑1 (MQ=255) gTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCG‑AAAAGGGGTGATCCGAAAGGGTCACCCCttttt > 8:256821/1‑101 (MQ=255) caccacCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCAccc > 7:162822/1‑85 (MQ=255) caccacCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCAccc < 8:162822/85‑1 (MQ=255) caccacCTTGACATGGTGGGGGTCGTTGGTTCGAGTCAAACTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTAACCCCTTTTTTCATTTTCCATTGCAGCGCGAGCGGGAAAACCGCGGCATGGACGCAAAGCGCTTTcta > 5:213619/1‑147 (MQ=37) actaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCTCTTTCAtt < 2:271437/146‑1 (MQ=255) accaccTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCAtt < 2:108467/149‑1 (MQ=255) aTGGTGGGGGTTGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGTGTGATCAGAAAGGGTCACCTCTTTTTTCATTGTCCATTTCCGCGCGAGCGGGAATAACTCGGCATGGACGCAAAGCGCTTTCATTCGAAAGttttt < 8:147535/149‑1 (MQ=37) aTGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGttttt < 3:155562/149‑1 (MQ=255) ttCTAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGGGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCaaaaaa < 7:108525/148‑1 (MQ=255) gTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCtt > 1:133069/1‑149 (MQ=255) gTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCACGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCtt < 6:180882/149‑1 (MQ=255) cAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGttttt > 3:217290/1‑124 (MQ=255) cAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGttttt < 4:217290/124‑1 (MQ=255) cAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTa > 4:151349/1‑148 (MQ=255) gCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGTGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTAc < 3:151349/148‑1 (MQ=255) gCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACg > 4:302767/1‑149 (MQ=255) ccTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTAc < 3:171666/141‑1 (MQ=255) ccTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTAc > 4:171666/1‑141 (MQ=255) aaaTCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGtttt < 3:297344/109‑1 (MQ=255) aaaTCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGtttt > 4:297344/1‑109 (MQ=255) aatgaaaaaaGGGGTGACCCTTTCGGATCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGaaa > 7:309768/6‑103 (MQ=25) aatgaaaaaaGGGGTGACCCTTTCGGATCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGaaa < 8:309768/98‑1 (MQ=25) gAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCAc < 1:88044/148‑1 (MQ=255) gAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCAc > 7:154006/1‑148 (MQ=255) aaaaaGGGGTGACCCTTTCGGATCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTC‑AAAAAGAGCTTTACAGGTACGCATCGGGCCACTaa > 1:157067/1‑149 (MQ=255) aaaaaGGGGTGACCCTTTCGGATCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTC‑AAAAAGAGCTTTACAGGTACGCATCGGGCCACTaa > 1:151723/1‑149 (MQ=255) ggTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATgcg > 7:280884/1‑149 (MQ=255) tGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGca > 6:346254/1‑149 (MQ=255) | AAAGTGTTGACAGGGTTTTTGTAATGCGTAGAATGCGCACCACACGACAGGCACATAGCTCAGTTGGTTAGAGCACCACCTTGACATGGTGGGGGTCGTTGGTTCGAGTCCAATTGTGCCTACCAAATCGAAAAAGGGGTGATCCGAAAGGGTCACCCCTTTTTTCATTGTCCATTGCCGCGCGAGCGGGAATACCGCGGCATGGACGCAAAGCGCTTTCATTCGAAAGTTTTTGATTTTTCTCAAAAAAGAGCTTTACAGGTACGCATCGGGCCACTAATATGCGCA > NC_002947/2796443‑2796730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |