Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,809,757 C→G 7.4% A55G (GCC→GGC)  thrS → threonine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,809,7570CG 7.4% 165.5 / 4.8 54A55G (GCC→GGC) thrSthreonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (23/27);  new base G (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

TTCCCGATGGCAGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGATCGGCCCGGTCATTGACGAAGGCTTCTATTAC  >  NC_002947/2809610‑2809905
                                                                                                                                                   |                                                                                                                                                    
ttCCCGATGGCAGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGc                                                                                                                                                      >  6:276403/1‑148 (MQ=255)
ttCCCGATGGCAGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGc                                                                                                                                                      >  4:57528/1‑148 (MQ=255)
  cccGATGGCAGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGc                                                                                                                                                      <  5:276403/146‑1 (MQ=255)
   ccGATGGCAGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCAcc                                                                                                                                                  >  8:171576/1‑149 (MQ=255)
      aTGGCAGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTg                                                                                                                                               <  3:71556/149‑1 (MQ=255)
           aGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGat                                                                                                                                          <  1:335568/149‑1 (MQ=255)
           aGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGat                                                                                                                                          <  2:212776/149‑1 (MQ=255)
            gTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGatc                                                                                                                                         <  3:57528/149‑1 (MQ=255)
                aCGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGAtcatca                                                                                                                                     >  2:206373/1‑149 (MQ=255)
                  gttcgttcGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGGCACCCTGCAGATCATCAcc                                                                                                                                   >  7:171414/1‑149 (MQ=255)
                  gttcgttcGATCACGACGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGGCACCCTGCAGATCATCAcc                                                                                                                                   >  3:173726/1‑149 (MQ=255)
                   ttcgttcgATCACACCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCAccc                                                                                                                                  <  1:65820/149‑1 (MQ=255)
                           aTCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGatcat                                                                                                                                       >  1:222682/1‑136 (MQ=255)
                           aTCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGatcat                                                                                                                                       <  2:222682/136‑1 (MQ=255)
                           aTCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGAt                                                                                                                          >  1:154016/1‑149 (MQ=255)
                           aTCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGAt                                                                                                                          >  1:203016/1‑149 (MQ=255)
                              aCGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGaa                                                                                                                       <  7:171576/149‑1 (MQ=255)
                                        cGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCATCAACGACGGCACCATGCAGATCATCACCCCTAAAGATGAAGAGGGACTg                                                                                                              <  4:173726/148‑1 (MQ=255)
                                        cGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGGCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTg                                                                                                              <  8:171414/148‑1 (MQ=255)
                                           aGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTg                                                                                                              >  1:214702/1‑145 (MQ=255)
                                            gCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTg                                                                                                              >  8:150125/1‑144 (MQ=255)
                                            gCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTg                                                                                                              >  6:48213/1‑144 (MQ=255)
                                             ccGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGAt                                                                                                         >  5:282511/1‑148 (MQ=255)
                                              cGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTg                                                                                                              >  3:285981/1‑142 (MQ=255)
                                              cGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTg                                                                                                              <  4:285981/142‑1 (MQ=255)
                                                 aGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTc                                                                                                    <  6:282511/149‑1 (MQ=255)
                                                 aGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTc                                                                                                    <  5:48213/149‑1 (MQ=255)
                                                      ccGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCAc                                                                                               <  1:206373/149‑1 (MQ=255)
                                                       cGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACt                                                                                              <  2:154016/149‑1 (MQ=255)
                                                        gCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGc                                                                                                                                              >  4:63096/1‑100 (MQ=255)
                                                        gCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGc                                                                                                                                              <  3:63096/100‑1 (MQ=255)
                                                          ttCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAag                                                                                                                      <  7:117508/122‑1 (MQ=255)
                                                          ttCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAag                                                                                                                      >  8:117508/1‑122 (MQ=255)
                                                              aTCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTgcgc                                                                                       >  6:352436/1‑149 (MQ=255)
                                                               tCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTgc                                                                                         <  7:179833/146‑1 (MQ=255)
                                                               tCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTgc                                                                                         >  8:179833/1‑146 (MQ=255)
                                                               tCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGcc                                                                                      <  8:302350/149‑1 (MQ=255)
                                                                cGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGcc                                                                                      >  2:149329/1‑148 (MQ=255)
                                                                  gcgcTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCAc                                                                                   <  1:149329/149‑1 (MQ=255)
                                                                  gcgcTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCAc                                                                                   <  7:150125/149‑1 (MQ=255)
                                                                         cctggccAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACttggttg                                                                            <  2:214702/149‑1 (MQ=255)
                                                                          ctggccAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACttggttgg                                                                           >  4:158888/1‑149 (MQ=255)
                                                                               cAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGTTCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCaag                                                                      >  6:9911/1‑147 (MQ=255)
                                                                                     cACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGGCCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTg                                                                 >  1:307760/1‑148 (MQ=255)
                                                                                              cGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCGCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGt                                                       <  2:203016/149‑1 (MQ=255)
                                                                                              cGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTg                                                        >  6:60515/1‑148 (MQ=255)
                                                                                                            gCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGcc                                            >  7:299789/1‑146 (MQ=255)
                                                                                                             cAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACt                                                                                  >  7:148614/1‑107 (MQ=255)
                                                                                                             cAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACt                                                                                  <  8:148614/107‑1 (MQ=255)
                                                                                                               aGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATg                                      <  8:299789/149‑1 (MQ=255)
                                                                                                                gCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATGACCCCTAATGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATgg                                     <  5:364179/149‑1 (MQ=255)
                                                                                                                    gTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGAt                                 <  6:338410/149‑1 (MQ=255)
                                                                                                                        aTGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCACCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGATCGGc                             >  2:111890/1‑149 (MQ=255)
                                                                                                                                  ccTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGATCGGCCCGGTCATTg                   <  3:158888/149‑1 (MQ=255)
                                                                                                                                          gCAACGACGCCACCCTGCAGTTCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGATCGGCCCGGTCATTGACGAAggt           <  5:9911/149‑2 (MQ=255)
                                                                                                                                            aaCGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAag                                                              <  6:46565/96‑1 (MQ=255)
                                                                                                                                            aaCGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAag                                                              >  5:46565/1‑96 (MQ=255)
                                                                                                                                                   ccACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGATCGGCCCGGTCATTGACGAAGGCTTCTATTAc  <  5:352436/149‑1 (MQ=255)
                                                                                                                                                   ccACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGATCGGCCCGGTCATTGACGAAGGCTTCTATTAc  >  2:169802/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCCCGATGGCAGTCAACGTTCGTTCGATCACGCCGTATCCGTAGCCGAAGTAGCCGCTTCCATCGGCGCTGGCCTGGCCAAGGCCACCGTTGCCGGCAAGGTCGACGGCAAGCTGGTCGATGCGTGTGACCTGATCAGCAACGACGCCACCCTGCAGATCATCACCCCTAAAGATGAAGAGGGACTGGAGATCATTCGTCACTCGTGCGCCCACTTGGTTGGCCACGCCGTGAAGCAGCTGTACCCGACCGCCAAGATGGTGATCGGCCCGGTCATTGACGAAGGCTTCTATTAC  >  NC_002947/2809610‑2809905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑