| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,841,936 | T→A | 17.8% | intergenic (‑115/+137) | yqhD ← / ← PP_2493 | NAD(P)‑dependent alcohol dehydrogenase/RadC family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,841,936 | 0 | T | A | 17.8% | 71.3 / 7.3 | 33 | intergenic (‑115/+137) | yqhD/PP_2493 | NAD(P)‑dependent alcohol dehydrogenase/RadC family protein |
| Reads supporting (aligned to +/- strand): ref base T (12/15); new base A (3/3); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAG > NC_002947/2841792‑2842078 | cgTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAgcttgctt < 5:86247/149‑1 (MQ=255)cgTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAgcttgctt < 6:319227/149‑1 (MQ=255) gggTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCAAGCTTCTaa < 1:345846/149‑1 (MQ=255) ggTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCAAGCTTCTaac < 8:94014/149‑2 (MQ=255) gTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAg < 3:254651/149‑1 (MQ=255) ggAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCAAGCTTCTaac > 1:213855/1‑142 (MQ=255) ggAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCAAGCTTCTaac < 2:213855/143‑2 (MQ=255) tCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGt > 4:242109/1‑149 (MQ=255) aaaaTTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGtt < 5:65763/148‑1 (MQ=255) ttGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGgtgt < 3:242109/149‑1 (MQ=255) gAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGtgtg < 2:265511/148‑1 (MQ=255) aGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTgg > 6:48639/1‑148 (MQ=255) aTAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCAt > 1:347657/1‑148 (MQ=255) cTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGt > 8:280411/1‑121 (MQ=255) cTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGt < 7:280411/121‑1 (MQ=255) aaaGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAgctt < 2:150336/97‑1 (MQ=255) aaaGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAgctt > 1:150336/1‑97 (MQ=255) gTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCt < 4:215549/131‑1 (MQ=255) tCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTc < 4:286208/128‑1 (MQ=255) tCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTc > 3:286208/1‑128 (MQ=255) tttttGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTc > 6:280101/1‑110 (MQ=255) tttttGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTc < 5:280101/110‑1 (MQ=255) tttttATTGCAGAAGCTCGTAGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCATGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGc > 7:135158/1‑149 (MQ=255) tttATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCt < 8:135158/148‑1 (MQ=255) cAGAAGCTCCTCGCCGCGCATTGCGCACCAAATAGCTGAAGATACGTTAGAAGCTAGCTTCTAAAGGCAACCAAGATGTTGTCGCAATCGTCAGCATTCATGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCcgcgcca > 6:184823/1‑147 (MQ=255) gCTCGTCGGCGCGCATTGAGCACCAAATAGATGAATATTGGTTAGAATCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTTCCGCCAATCAGCGTAAGCTCCGCGAGATGTc > 4:309996/1‑148 (MQ=255) gtcgGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAg > 1:209081/1‑107 (MQ=255) gtcgGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAg < 2:209081/107‑1 (MQ=255) gcACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGCCATTCGTCTGCATTCCTGGTCCCGAGACAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCTCGCGATGTCGAGGGTTGTTGCTAGGCACt > 6:167268/1‑149 (MQ=255) ccAAATAGCTGTAGATTGTTTAGAAGCTTGGTTCTAAAGGCTAGCTATTTGGTGTGTCATTCGTCTGAATTCCTGGTACCGTGCCAAAGTCGTTGCCGCCAAGCATCGTAATCTCCTAGCGATTTCGAGGGTTGTTGCTAGGCACtga < 6:94279/148‑1 (MQ=255) tcTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTTGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAATGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCTCGATGTCGAGGGTTGTTGCTAGGCTCTGATGATAgg < 5:167268/147‑1 (MQ=255) gAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCAt > 3:31173/1‑149 (MQ=255) gttgcctttAGAAGCAAGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCg > 8:1430/8‑149 (MQ=255) gATTGGTTATAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCgg < 3:309996/149‑1 (MQ=255) ttAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAg > 5:89521/1‑122 (MQ=255) ttAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAg < 6:89521/122‑1 (MQ=255) gatgcttgcttCTAAAGGCAACCAAGTTGGGGTTGCATTCGTCTGCATTTCTGGTGCCGAGGCAATGGCTTTGCCGGCAAGCAGCGTAAGCTGCGCGCGATGTCGTGGGTTGTTGCTAGTGACTGTTGATTGGGCATCGGGAGTCATa < 5:184823/145‑1 (MQ=255) gAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAg < 5:48639/149‑1 (MQ=255) | CGTGGGGTTGTGGAAGTCAAAATTGAGCATAGATTTCTCTACTCCTTAAAAGTAGACCAGTCGTATATTTTTTGGTGAAATTTTTATTGCAGAAGCTCGTCGGCGCGCATTGCGCACCAAATAGCTGAAGATTGGTTAGAAGCTTGCTTCTAAAGGCAACCAAGTTGGTGTGGCATTCGTCTGCATTCCTGGTCCCGAGCCAAAGGCGTTGCCGCCAAGCAGCGTAAGCTCCGCGCGATGTCGAGGGTTGTTGCTAGGCACTGATGATAGGGCATCGGGAGTCATAG > NC_002947/2841792‑2842078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |