Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,852,406 A→C 7.7% L309R (CTG→CGG) ‡ PP_2505 ← GAF domain/GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,852,4060AC 7.7% 143.2 / 2.6 49L309R (CTG→CGG) ‡PP_2505GAF domain/GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (27/18);  new base C (2/2);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTCCTCTCCTCC  >  NC_002947/2852261‑2852554
                                                                                                                                                 |                                                                                                                                                    
cGTACCCTGCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCAGGGTGAGCAga                                                                                                                                                    <  8:119329/148‑2 (MQ=255)
 gTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATTACGCTGGCACATAGTCAACTTGGTCACGTGCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGgc                                                                                                                                                  <  8:93151/149‑1 (MQ=255)
     ccTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCAc                                                                                                                                              >  5:153396/1‑149 (MQ=255)
       tCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCAcc                                                                                                                                             >  4:292371/1‑148 (MQ=255)
                cGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCAt                                                                                                                                   <  6:153396/149‑1 (MQ=255)
                  gagaTCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCa                                                                                                                                           >  6:163722/1‑139 (MQ=255)
                  gagaTCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCa                                                                                                                                           <  5:163722/139‑1 (MQ=255)
                   agaTCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGt                                                                                                                                >  4:229878/1‑149 (MQ=255)
                       cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTc                                                                                                                               >  8:113689/1‑146 (MQ=255)
                       cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTc                                                                                                                               <  7:113689/146‑1 (MQ=255)
                       cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACAATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATc                                                                                                                            >  7:122474/1‑149 (MQ=255)
                        ttAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAACCTGCTCACCCAGGGTGAGCAgat                                                                                                                                                   >  7:274554/1‑123 (MQ=255)
                        ttAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAACCTGCTCACCCAGGGTGAGCAgat                                                                                                                                                   <  8:274554/125‑3 (MQ=255)
                             ttCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCagg                                                                                                                                         <  3:9155/130‑3 (MQ=255)
                             ttCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACAGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCagg                                                                                                                                         >  4:9155/1‑128 (MQ=255)
                               ccTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCa                                                                                                                                               <  8:142449/122‑1 (MQ=255)
                               ccTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCa                                                                                                                                               >  7:142449/1‑122 (MQ=255)
                               ccTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGa                                                                                                                      <  3:292371/147‑1 (MQ=255)
                                         aCGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGAATAGAGACCGCCGTCAGAGAGCTTTATTGCATCATAAATCTGCTCACCCTGCGTGAGCATTGCTCACACAATGCTCATGGTCATCTTCCGATGGCCGCCggt                                                                                                           >  8:332402/1‑147 (MQ=255)
                                                       aCAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGgcgc                                                                                            >  4:230690/1‑149 (MQ=255)
                                                         aaCTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGgcgcgc                                                                                          >  8:146064/1‑149 (MQ=255)
                                                         aaCTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTAATGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACCGCACCGgcgcgc                                                                                          >  8:290894/1‑149 (MQ=255)
                                                              ggTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGc                                                                                       <  3:229878/147‑1 (MQ=255)
                                                                   cGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCCCCCTGGGGGAGCAGGGCGCACCCAATCCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtga                                                                                <  4:293300/149‑1 (MQ=255)
                                                                      ccaccaTGTTTGGCCGCATAGAGACAGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGTAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtgact                                                                              >  6:359336/1‑148 (MQ=255)
                                                                                     gCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCg                                                               >  4:156711/1‑148 (MQ=255)
                                                                                      cATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGAGCGCTGCCGATTGACTGACGCAAGCGCTCCGc                                                              >  7:339486/1‑148 (MQ=255)
                                                                                          gagaCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCtt                                                                                                                          <  8:45001/84‑1 (MQ=255)
                                                                                          gagaCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCtt                                                                                                                          >  7:45001/1‑84 (MQ=255)
                                                                                            gaCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc                                                       <  3:230690/149‑1 (MQ=255)
                                                                                             aCCGCCGTCAGCGCGATTTATTGCAGGATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAAGAGTAACTGCGCGCTGCCGATTGACTGACGCAAGCGCTTCGCAATCTgc                                                       <  8:122474/148‑1 (MQ=255)
                                                                                              ccgccgTCAGCGCGCTTTATTTCATCATCAATCTGCTCACCCTGGGTGAGCAGTGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgcg                                                      <  7:332402/148‑1 (MQ=255)
                                                                                                 ccgTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCaa                                                  >  3:71831/1‑149 (MQ=255)
                                                                                                         gcgcTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGc                                          >  4:295387/1‑149 (MQ=255)
                                                                                                                aTTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCtt                                    <  3:156711/148‑1 (MQ=255)
                                                                                                                     atcatcAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAAccc                              >  2:326178/1‑149 (MQ=255)
                                                                                                                       catcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc                                                       <  4:33670/122‑1 (MQ=255)
                                                                                                                       catcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc                                                       >  3:33670/1‑122 (MQ=255)
                                                                                                                       catcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCg                             >  1:64504/1‑148 (MQ=255)
                                                                                                                        atcaATCTGCTCACCCTGGGTGAGCAGGGCGCTCCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCg                             >  8:155221/1‑147 (MQ=255)
                                                                                                                           aaTCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGTCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGc                         >  7:82535/1‑148 (MQ=255)
                                                                                                                            cccTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGc                         >  6:88184/3‑147 (MQ=255)
                                                                                                                              cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACTCGTAGccttg                     >  8:295912/1‑145 (MQ=255)
                                                                                                                                gCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGc                    >  6:119061/1‑148 (MQ=255)
                                                                                                                                    aCCCTGGGTGAGTAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGTCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTAc                 <  5:359336/147‑1 (MQ=255)
                                                                                                                                    aCCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACg                >  1:168124/1‑148 (MQ=255)
                                                                                                                                        tcggTGAGAAGGGCGCACCCAAAGCTAATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAAAGGCCCGATGCCGATTGACTCACGCAAGCGCTCCGCAATCTGCGCCAATACTAGGCTATCTTTAACCCGTAGAAACGATACGAAtt            >  8:334456/3‑148 (MQ=255)
                                                                                                                                          ggTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATtc           <  2:168124/147‑1 (MQ=255)
                                                                                                                                                cAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATtcctctcctc   <  3:295387/149‑1 (MQ=255)
                                                                                                                                                 aGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGTCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTCCTCtcctcc  <  8:82535/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTCCTCTCCTCC  >  NC_002947/2852261‑2852554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑