Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,852,408 G→T 12.4% A308A (GCC→GCA)  PP_2505 ← GAF domain/GGDEF domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,852,4080GT12.4% 129.2 / 5.0 47A308A (GCC→GCA) PP_2505GAF domain/GGDEF domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (25/16);  new base T (3/3);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTCCTCTCCTCC  >  NC_002947/2852262‑2852554
                                                                                                                                                  |                                                                                                                                                  
gTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATTACGCTGGCACATAGTCAACTTGGTCACGTGCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGgc                                                                                                                                                  <  8:93151/149‑1 (MQ=255)
    ccTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCAc                                                                                                                                              >  5:153396/1‑149 (MQ=255)
      tCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCAcc                                                                                                                                             >  4:292371/1‑148 (MQ=255)
               cGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCAt                                                                                                                                   <  6:153396/149‑1 (MQ=255)
                 gagaTCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCa                                                                                                                                           >  6:163722/1‑139 (MQ=255)
                 gagaTCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCa                                                                                                                                           <  5:163722/139‑1 (MQ=255)
                  agaTCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGt                                                                                                                                >  4:229878/1‑149 (MQ=255)
                      cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTc                                                                                                                               >  8:113689/1‑146 (MQ=255)
                      cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTc                                                                                                                               <  7:113689/146‑1 (MQ=255)
                      cTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACAATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATc                                                                                                                            >  7:122474/1‑149 (MQ=255)
                            ttCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCagg                                                                                                                                         <  3:9155/130‑3 (MQ=255)
                            ttCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACAGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGCGCCCTGCTCACCCagg                                                                                                                                         >  4:9155/1‑128 (MQ=255)
                              ccTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCa                                                                                                                                               <  8:142449/122‑1 (MQ=255)
                              ccTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCa                                                                                                                                               >  7:142449/1‑122 (MQ=255)
                              ccTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGa                                                                                                                      <  3:292371/147‑1 (MQ=255)
                                        aCGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGAATAGAGACCGCCGTCAGAGAGCTTTATTGCATCATAAATCTGCTCACCCTGCGTGAGCATTGCTCACACAATGCTCATGGTCATCTTCCGATGGCCGCCggt                                                                                                           >  8:332402/1‑147 (MQ=255)
                                                      aCAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGgcgc                                                                                            >  4:230690/1‑149 (MQ=255)
                                                        aaCTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGgcgcgc                                                                                          >  8:146064/1‑149 (MQ=255)
                                                        aaCTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTAATGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACCGCACCGgcgcgc                                                                                          >  8:290894/1‑149 (MQ=255)
                                                             ggTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGc                                                                                       <  3:229878/147‑1 (MQ=255)
                                                                  cGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCCCCCTGGGGGAGCAGGGCGCACCCAATCCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtga                                                                                <  4:293300/149‑1 (MQ=255)
                                                                     ccaccaTGTTTGGCCGCATAGAGACAGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGTAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATtgact                                                                              >  6:359336/1‑148 (MQ=255)
                                                                                    gCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCg                                                               >  4:156711/1‑148 (MQ=255)
                                                                                     cATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGAGCGCTGCCGATTGACTGACGCAAGCGCTCCGc                                                              >  7:339486/1‑148 (MQ=255)
                                                                                         gagaCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCtt                                                                                                                          >  7:45001/1‑84 (MQ=255)
                                                                                         gagaCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCtt                                                                                                                          <  8:45001/84‑1 (MQ=255)
                                                                                           gaCCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc                                                       <  3:230690/149‑1 (MQ=255)
                                                                                            aCCGCCGTCAGCGCGATTTATTGCAGGATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAAGAGTAACTGCGCGCTGCCGATTGACTGACGCAAGCGCTTCGCAATCTgc                                                       <  8:122474/148‑1 (MQ=255)
                                                                                             ccgccgTCAGCGCGCTTTATTTCATCATCAATCTGCTCACCCTGGGTGAGCAGTGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgcg                                                      <  7:332402/148‑1 (MQ=255)
                                                                                                ccgTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCaa                                                  >  3:71831/1‑149 (MQ=255)
                                                                                                        gcgcTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGc                                          >  4:295387/1‑149 (MQ=255)
                                                                                                               aTTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCtt                                    <  3:156711/148‑1 (MQ=255)
                                                                                                                    atcatcAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAAccc                              >  2:326178/1‑149 (MQ=255)
                                                                                                                      catcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc                                                       <  4:33670/122‑1 (MQ=255)
                                                                                                                      catcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTgc                                                       >  3:33670/1‑122 (MQ=255)
                                                                                                                      catcaATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCg                             >  1:64504/1‑148 (MQ=255)
                                                                                                                       atcaATCTGCTCACCCTGGGTGAGCAGGGCGCTCCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCg                             >  8:155221/1‑147 (MQ=255)
                                                                                                                          aaTCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGTCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGc                         >  7:82535/1‑148 (MQ=255)
                                                                                                                           cccTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGc                         >  6:88184/3‑147 (MQ=255)
                                                                                                                             cTGCTCACCCAGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACTCGTAGccttg                     >  8:295912/1‑145 (MQ=255)
                                                                                                                               gCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGc                    >  6:119061/1‑148 (MQ=255)
                                                                                                                                   aCCCTGGGTGAGTAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGTCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTAc                 <  5:359336/147‑1 (MQ=255)
                                                                                                                                   aCCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACg                >  1:168124/1‑148 (MQ=255)
                                                                                                                                       tcggTGAGAAGGGCGCACCCAAAGCTAATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAAAGGCCCGATGCCGATTGACTCACGCAAGCGCTCCGCAATCTGCGCCAATACTAGGCTATCTTTAACCCGTAGAAACGATACGAAtt            >  8:334456/3‑148 (MQ=255)
                                                                                                                                         ggTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATtc           <  2:168124/147‑1 (MQ=255)
                                                                                                                                               cAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATtcctctcctc   <  3:295387/149‑1 (MQ=255)
                                                                                                                                                aGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGTCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTCCTCtcctcc  <  8:82535/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTACCCTCCCGGATCCGGAGATCTTAACTTCCTCAGGATAACGCTGGCACATAGACAACTTGGTCACGTCCACCATGTTTGGCCGCATAGAGACCGCCGTCAGCGCGCTTTATTGCATCATCAATCTGCTCACCCTGGGTGAGCAGGGCGCACCCAATGCTCATGGTCATCTTCCGAGGGCCGCCGGGTAACAGAAACGGCGCGCTGCCGATTGACTGACGCAAGCGCTCCGCAATCTGCGCCAATTCTTGGCTATCTTTAACCCGTAGCAAGGCTACGAATTCCTCTCCTCC  >  NC_002947/2852262‑2852554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑