Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,859,150	T→C	6.3%	C127C (TGT→TGC) ‡	PP_2511 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,859,150	0	T	C	6.3%	226.8 / 4.1	79	C127C (TGT→TGC) ‡	PP_2511	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/40);  new base C (3/2);  total (37/42)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGTTGGCGTGCTGCCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGCAACGG  >  NC_002947/2859003‑2859292                                                                                                                                                      |                                                                                                                                                gAGTTGGCGTGCTGCCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGt                                                                                                                                                 <  6:310084/148‑1 (MQ=255)        cGTGCTGCCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTga                                                                                                                                         <  7:168951/149‑1 (MQ=255)         gTGCTGCCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTgag                                                                                                                                        <  7:204172/149‑1 (MQ=255)         gTGCTGCCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTgag                                                                                                                                        <  5:175620/149‑1 (MQ=255)             tgcCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGt                                                                                                                                    >  7:114639/1‑149 (MQ=255)              gcCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTc                                                                                                                                   >  6:241874/1‑149 (MQ=255)                 gATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCa                                                                                                                                 >  5:326621/1‑148 (MQ=255)                      gggCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGt                                                                                                                            >  7:209641/1‑148 (MQ=255)                        gCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTca                                                                                                                          >  4:217027/1‑148 (MQ=255)                             tgGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTc                                                                                                                     <  8:74676/148‑1 (MQ=255)                                 tCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAg                                                                                                                <  5:241874/149‑1 (MQ=255)                                    cgcCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTTTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAgac                                                                                                              >  2:55477/1‑146 (MQ=255)                                     gcCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGa                                                                                                            <  2:48812/149‑1 (MQ=255)                                       cACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGc                                                                                                          <  8:326851/149‑1 (MQ=255)                                            gCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTa                                                                                                      <  6:317342/148‑1 (MQ=255)                                            gCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTa                                                                                                      >  5:317342/1‑148 (MQ=255)                                              ttGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCAACGTTGTAACGGTGACGAGTCCAAATGTCACATTCGTTAGCCAAGAACTACCa                                                                                                   >  6:235166/1‑149 (MQ=255)                                                ggCGTGCGCTACGTCCCCTGTTTTCGGTTGTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCAt                                                                                                  <  8:211980/148‑1 (MQ=255)                                                        cTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTg                                                                                         <  3:217027/149‑1 (MQ=255)                                                        cTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTg                                                                                         <  3:75716/149‑1 (MQ=255)                                                           cGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGt                                                                                       <  2:188502/148‑1 (MQ=255)                                                             tCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGGTACCTCATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGc                                                                                                               <  5:334382/122‑1 (MQ=255)                                                             tCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCa                                                                                                         <  6:129846/128‑1 (MQ=255)                                                             tCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCa                                                                                                         >  5:129846/1‑128 (MQ=255)                                                             tCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGCCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGACACATTCCTTAGc                                                                                                               >  6:334382/1‑122 (MQ=255)                                                               cccTGTTTTCGGTTCTTTACAGGCTGGTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCg                                                                                     >  1:4549/1‑147 (MQ=255)                                                               cccTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCg                                                                                     <  2:4549/146‑1 (MQ=255)                                                               cccTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTa                                                                                  <  6:326621/149‑1 (MQ=255)                                                                    ttttCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGt                                                                             <  8:114639/149‑1 (MQ=255)                                                                       tCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTAcc                                                                                                    >  8:266665/1‑123 (MQ=255)                                                                       tCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTAcc                                                                                                    <  7:266665/123‑1 (MQ=255)                                                                               ttACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTg                                                                            <  5:75432/139‑1 (MQ=255)                                                                               ttACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTg                                                                            >  6:75432/1‑139 (MQ=255)                                                                                tACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCg                                                                   <  8:209641/146‑1 (MQ=255)                                                                                  cAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAgcg                                                              >  4:174416/1‑149 (MQ=255)                                                                                   aGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAgcgc                                                             >  5:220093/1‑149 (MQ=255)                                                                                    ggCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAgcgc                                                             >  5:80243/1‑148 (MQ=255)                                                                                     gCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCa                                                                 >  1:212050/1‑143 (MQ=255)                                                                                     gCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCa                                                                 <  2:212050/143‑1 (MQ=255)                                                                                     gCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGcgcg                                                            >  4:29640/1‑148 (MQ=255)                                                                                       t‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTg                                                                            <  3:188638/131‑1 (MQ=255)                                                                                       t‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTg                                                                            >  4:188638/1‑131 (MQ=255)                                                                                       cggTTCTGTCGTTTTAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGGCGGTATTGGTGA‑AAACCCGCAGCGCGaa                                                          <  8:54375/147‑1 (MQ=255)                                                                                          ttCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAggg                                                       <  3:174416/149‑1 (MQ=255)                                                                                          ttCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAggg                                                       >  2:32146/1‑149 (MQ=255)                                                                                                cGATTGAGGACACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCt                                                  <  1:55477/148‑1 (MQ=255)                                                                                                   ttGAGTCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGTGCACCGTTGTAACGGTGAGTAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTg                                                                               <  2:229616/117‑1 (MQ=255)                                                                                                   ttGAGGCCACACTTGGCCTGGCTACCACATCCCTGATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTg                                                                               >  1:229616/1‑118 (MQ=255)                                                                                                     gAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTg                                                                               <  4:311012/115‑1 (MQ=255)                                                                                                     gAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTg                                                                               >  3:311012/1‑115 (MQ=255)                                                                                                      aGGCCACACTTGGCCTGGCAACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGTGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGGATTGGTGATAAACCCGCAGCGCGAAGGGCTTCTGGacac                                            <  1:32146/149‑1 (MQ=255)                                                                                                       ggCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGt                                                                             <  8:26034/115‑1 (MQ=255)                                                                                                       ggCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGt                                                                             >  7:26034/1‑115 (MQ=255)                                                                                                           acacTTGGCCTGGCTACGACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCg                                      <  5:235166/149‑1 (MQ=255)                                                                                                           acacTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCg                                      <  3:29640/149‑1 (MQ=255)                                                                                                                  gCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCg                               <  6:220093/149‑1 (MQ=255)                                                                                                                   ccTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTTA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCgg                              <  6:148370/149‑1 (MQ=255)                                                                                                                    cTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTAc                                                                                                     <  7:261323/78‑1 (MQ=255)                                                                                                                    cTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTAc                                                                                                     >  8:261323/1‑78 (MQ=255)                                                                                                                    cTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCgg                              >  4:162759/1‑148 (MQ=255)                                                                                                                          aCCAAATCCC‑GATGCCGGGCACCGTTGTAACGGTGAGGAGTCAAAATGTCAAATTCGTTAGCCGAGCACTAACATGAAAACCTGGTCGGTATTCGTGA‑AAACCCGAAGCGCGCAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAga                       >  5:250894/1‑148 (MQ=255)                                                                                                                            cacaTCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGa‑aaa                                                                       <  3:195401/99‑1 (MQ=255)                                                                                                                            cacaTCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGa‑aaa                                                                       >  4:195401/1‑99 (MQ=255)                                                                                                                               aTCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGAt                    <  5:137890/147‑1 (MQ=255)                                                                                                                               aTCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGAt                    >  6:137890/1‑147 (MQ=255)                                                                                                                                tCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCg                               >  7:14628/1‑135 (MQ=255)                                                                                                                                tCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCg                               <  8:14628/135‑1 (MQ=255)                                                                                                                                      aTGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAACGCGTTGAGGTACt            >  8:293986/1‑149 (MQ=255)                                                                                                                                        actcctcACCGTTACAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTtg          >  8:292918/7‑149 (MQ=255)                                                                                                                                          tcctcACCGTTACAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTtgtg        >  6:355870/5‑149 (MQ=255)                                                                                                                                          cGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCt                                                  >  4:103325/1‑107 (MQ=255)                                                                                                                                          cGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCt                                                  <  3:103325/107‑1 (MQ=255)                                                                                                                                           gggCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTtgtg        >  4:149158/1‑148 (MQ=255)                                                                                                                                           gggCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGc       <  6:80243/149‑1 (MQ=255)                                                                                                                                            ggCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGc       >  5:147916/1‑148 (MQ=255)                                                                                                                                             tcACCGTTACAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGc                                >  5:81717/2‑122 (MQ=255)                                                                                                                                             tcACCGTTACAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGc                                <  6:81717/121‑1 (MQ=255)                                                                                                                                             gCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGt                                  <  3:88215/120‑1 (MQ=255)                                                                                                                                             gCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGt                                  >  4:88215/1‑120 (MQ=255)                                                                                                                                                ccGTTACAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGCAACgg  <  7:292918/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                GAGTTGGCGTGCTGCCGATGTGGGCCTGTGGGTCGCGCCACTGGCTTGGCGTCCGCTACGTCCCCTGTTTTCGGTTCTTTACAGGCT‑GTTCTGTCGATTGAGGCCACACTTGGCCTGGCTACCACATCCC‑GATGGCGGGCACCGTTGTAACGGTGAGGAGTCCAAATGTCACATTCGTTAGCCGAGCACTACCATGAAATCCTGGTCGGTATTGGTGA‑AAACCCGCAGCGCGAAGGGCTTCTGGACACCCCCCGGCGTGCGGCAAAAGCGATGCAGTACTTGTGCAACGG  >  NC_002947/2859003‑2859292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑