Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,863,829 T→G 11.4% S297A (TCG→GCG)  gllR → HTH‑type transcriptional regulator GalR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,863,8290TG11.4% 87.0 / 4.2 43S297A (TCG→GCG) gllRHTH‑type transcriptional regulator GalR
Reads supporting (aligned to +/- strand):  ref base T (12/23);  new base G (3/2);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

CCTCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTGCGCACCAGTTGGC  >  NC_002947/2863682‑2863977
                                                                                                                                                   |                                                                                                                                                    
ccTCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTc                                                                                                                                                     <  1:45777/149‑1 (MQ=255)
           tGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGTAAATGGTGGCACTGGCCCGTCGGGGGCACCCCCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCGAGCCGGCCCg                                                                                                                                            <  1:116072/147‑1 (MQ=255)
           tGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGt                                                                                                                                           <  3:206148/148‑1 (MQ=255)
           tGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCGAGCCGGCCCgcg                                                                                                                                          <  6:338124/149‑3 (MQ=255)
              cAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAACTGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAgc                                                                                                                                       <  1:235082/149‑1 (MQ=255)
                      tggtggGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAg                                                                                                                                        <  1:47959/140‑1 (MQ=255)
                        gtggGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGAc                                                                                                                               <  6:18196/147‑1 (MQ=255)
                         ggggCGAGCCGCGCATCAACGAGGAAATGGTGGTGCGGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAAc                                                                                                                            <  4:55080/148‑1 (MQ=255)
                               aGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTc                                                                                                                      <  3:240033/149‑1 (MQ=255)
                                          aaCGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCCGCTGCTGGACAACAGCGTCGCCGCCGCggg                                                                                                           >  3:346009/1‑149 (MQ=255)
                                           aCGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACACTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGc                                                                                                          <  5:336892/149‑1 (MQ=255)
                                                               gCCCGTCGGGGGCACCCGCTGTTGCTCACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGAGAACTGCTTcgc                                                                                                                    <  5:347942/119‑1 (MQ=255)
                                                               gCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCGCGCCGGCCCGTCCCCAGCGCGCCAACAGCGTCGCCCCCGCCGGGCTGGCCGCGCCCTGGcccgg                                                                                      >  7:294431/1‑146 (MQ=255)
                                                                 ccGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCggtgg                                                                                    <  1:56040/149‑1 (MQ=255)
                                                                        gggCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCACGCCGCCCCCTCAGCTGCTCGACAACAGCTTCGCCGCCGCGGGCCGGGCAGCGGCGTGGCCGGTGGGGGAAAg                                                                             >  6:336995/1‑149 (MQ=255)
                                                                           gaCCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTGCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAgcgc                                                                          <  5:332019/148‑1 (MQ=255)
                                                                            aCCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCt                                                                         <  4:140628/149‑1 (MQ=255)
                                                                              ccGCTGTTGCACACTCACCTGACGCTCATGGGCGTGGACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGTCTACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCggtggt                                                                                   <  7:183476/137‑1 (MQ=255)
                                                                              ccGCTCTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCAGCGGCCCGGCACGCAGGCCCGACAGCAGCACGCCAACTGCTAACCCGCCGCCGGCCTGCCAGCCCCGAGGCAggtcga                                                                                   >  8:183476/1‑134 (MQ=255)
                                                                                      ctacacTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGCGGTGGAAAGCGCTGATCTGGCg                                                                <  7:54913/146‑1 (MQ=255)
                                                                                         cacTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCACGCCGGCCCGGCAGCTGCTCGACAACGGCGTAGCCGCCGCGGGCCGGGCAGCGCCGGGGCCGGTGGTGGAAAGCGCTGATCTGGCggg                                                              >  8:54913/1‑146 (MQ=255)
                                                                                                cTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTAGACAACAGCTTAGCCGCCGCGGGCCTGACAGCGCCGGGGCCGGTGGTGGAAAGCGCTGATCAGGCGGTTATcatttg                                                     >  2:148714/1‑144 (MQ=255)
                                                                                                 tGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGc                                                                 >  8:193553/1‑136 (MQ=255)
                                                                                                 tGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGc                                                                 <  7:193553/136‑1 (MQ=255)
                                                                                                      cTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGa                                                                                >  3:331627/1‑116 (MQ=255)
                                                                                                      cTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGa                                                                                <  4:331627/116‑1 (MQ=255)
                                                                                                       tCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTg                                              >  3:49515/1‑149 (MQ=255)
                                                                                                         aaGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGt                                            <  4:49515/149‑1 (MQ=255)
                                                                                                              cGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGtt                                       >  1:218204/1‑149 (MQ=255)
                                                                                                               gTGCACCAGGCGCGCTGGGTGTTGCGGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTc                                      <  8:294431/149‑1 (MQ=255)
                                                                                                                 gCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGGCGGTGGTGGAAATCGCTGATCTGGCGGGTATCCGTTGCCTACTGGTGCGTTCg                                     >  1:237263/1‑148 (MQ=255)
                                                                                                                        gcgcgcTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCt                             >  5:72043/1‑149 (MQ=255)
                                                                                                                                 gtgtTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGGGGCCGGTGGTGGTAAGCGCGGATGTGGGGGTTTTGCGTGGCCTACTGGTGGGTTGGGGCATGCTGGCGGCGgt                    >  2:222424/1‑149 (MQ=255)
                                                                                                                                    ttGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGgtgt                  <  2:218204/148‑1 (MQ=255)
                                                                                                                                       ccGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCGGGCGGCGGTGTCTgc              >  8:250690/1‑149 (MQ=255)
                                                                                                                                        gacgGGCCGGCGAGCCGGCCCGCCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGTCGGTTATCCGTGTCCTACtag                                             >  4:194332/3‑115 (MQ=255)
                                                                                                                                        cgcgGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTTGCGGCGGTGTCTgcg             <  2:308719/149‑1 (MQ=255)
                                                                                                                                        cgcgGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTgcg             <  4:346009/149‑1 (MQ=255)
                                                                                                                                         gcgGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTgcgc            >  1:144303/1‑149 (MQ=255)
                                                                                                                                              ccGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTgcgc            >  4:130176/1‑144 (MQ=255)
                                                                                                                                              ccGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTgcgc            <  3:130176/144‑1 (MQ=255)
                                                                                                                                               cGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTGCGCACCAGt      >  3:118944/1‑149 (MQ=255)
                                                                                                                                                ggCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTGCGCACCAGtt     <  4:118944/149‑1 (MQ=255)
                                                                                                                                                   tCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTGCGCACCAGTTGGc  >  5:227152/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCTCACGACTATGCCAGCGACCTGGTGGGCGAGCCGCTCATCAACGAGGAAATGGTGGTGCTGGCCCGTCGGGGGCACCCGCTGTTGCACACTCACCTGACGCTCAAGGGCGTGCACCAGGCGCGCTGGGTGTTGCCGCGGGCCGGCTCGCCGGCCCGTCAGCTGCTCGACAACTGCTTCGCCGCCGCGGGCCTGACAGCGCCGTGGCCGGTGGTGGAAAGCGCTGATCTGGCGGTTATCCGTGGCCTACTGGTGCGTTCGGACATGCTGGCGGCGGTGTCTGCGCACCAGTTGGC  >  NC_002947/2863682‑2863977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑