Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,868,876 G→C 8.4% intergenic (+415/‑516) PP_2520 → / → PP_2522 hypothetical protein/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,868,8760GC 8.4% 141.7 / 2.7 47intergenic (+415/‑516)PP_2520/PP_2522hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base G (19/24);  new base C (2/2);  total (21/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

AAAGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGC  >  NC_002947/2868728‑2869018
                                                                                                                                                    |                                                                                                                                              
aaaGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCg                                                                                                                                                >  3:269107/1‑149 (MQ=255)
 aaGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCgg                                                                                                                                               <  2:269894/149‑1 (MQ=255)
         cAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTgg                                                                                                                                       >  3:258471/1‑149 (MQ=255)
          aGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCACCAATGCCCCGGCATTgat                                                                                                                                      <  7:84292/149‑3 (MQ=255)
            ccTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTg                                                                                                                                        >  4:133941/1‑145 (MQ=255)
            ccTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTg                                                                                                                                        <  3:133941/145‑1 (MQ=255)
                gTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTg                                                                                                                                >  4:38039/1‑149 (MQ=255)
                gTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTg                                                                                                                                <  3:192825/149‑1 (MQ=255)
                gTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCAGGTGCATTGGTGATCTg                                                                                                                                >  3:316198/1‑149 (MQ=255)
                 ttCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGc                                                                                                                               <  2:271764/149‑1 (MQ=255)
                      gccattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCg                                                                                                                              >  8:18961/1‑145 (MQ=255)
                      gccattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCg                                                                                                                              <  7:18961/145‑1 (MQ=255)
                      gccattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCg                                                                                                                              <  1:71395/145‑1 (MQ=255)
                      gccattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCg                                                                                                                              >  2:71395/1‑145 (MQ=255)
                      gccattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGatat                                                                                                                          <  3:38039/149‑1 (MQ=255)
                        cattggccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGGTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatca                                                                                                                        <  6:257718/149‑1 (MQ=255)
                          tttgccattCGGGTGAAGCCATTTGGGAGCTGCTGGTGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTTGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATatcaat                                                                                                                      <  4:316198/146‑1 (MQ=255)
                             gccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCACCAATGCCCCGGCATtgatgaa                                                                                                                                   >  4:72659/1‑132 (MQ=255)
                             gccattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCACCAATGCCCCGGCATtgatgaa                                                                                                                                   <  3:72659/133‑2 (MQ=255)
                             gccattCGCGTGAAGCCATTTCGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCTTTCAACTCGTTGGTGCAGCTGCAGTACGAGCGCGGGCGGCCGCGTAACCCGTACATCAATTCCGGGCCATTCGTGATCTGCGATATAAATCAg                                                                                                                   >  8:150144/1‑149 (MQ=255)
                               cattCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTc                                                                                                                 >  1:47562/1‑149 (MQ=255)
                                              aTTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCt                                                                                                                                 <  6:189509/118‑1 (MQ=255)
                                              aTTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCt                                                                                                                                 >  5:189509/1‑118 (MQ=255)
                                                      gCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGt                                                                                          <  6:7139/149‑1 (MQ=255)
                                                       cGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTc                                                                                         <  4:269107/149‑1 (MQ=255)
                                                        ggCTGGGGCATGAGCCTTCGGGGCAGCCGTTGAACTCGTTGGTGCAGGTGGTGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGTTATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCg                                                                                        <  7:150144/149‑1 (MQ=255)
                                                        ggCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCg                                                                                        <  2:116433/149‑1 (MQ=255)
                                                                     gCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTg                                                                           <  2:105194/149‑1 (MQ=255)
                                                                      ccTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCg                                                                                                                                                <  3:164670/79‑1 (MQ=255)
                                                                      ccTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGt                                                                          <  4:258471/149‑1 (MQ=255)
                                                                      ccTTAGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCg                                                                                                                                                >  4:164670/1‑79 (MQ=255)
                                                                       cTTCGGCGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGAGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTc                                                                         >  1:63228/1‑149 (MQ=255)
                                                                          cGGGGCAGCCGTGCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTcc                                                                        <  7:311958/147‑1 (MQ=255)
                                                                           ggggCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGAc                                                                     <  2:33043/149‑1 (MQ=255)
                                                                              gCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGAc                                                                     <  6:42951/146‑1 (MQ=255)
                                                                              gCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGAc                                                                     >  5:42951/1‑146 (MQ=255)
                                                                              gCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGATGGc                                                                  >  1:105606/1‑149 (MQ=255)
                                                                                         aCTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAAc                                                       >  5:335727/1‑149 (MQ=255)
                                                                                          cTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGt                                                                                          >  1:305495/1‑113 (MQ=255)
                                                                                          cTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGt                                                                                          <  2:305495/113‑1 (MQ=255)
                                                                                          cTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAAcc                                                      <  2:47562/149‑1 (MQ=255)
                                                                                             gTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCt                                                    >  6:250737/1‑148 (MQ=255)
                                                                                                      gCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGa                                          >  3:162069/1‑149 (MQ=255)
                                                                                                       cTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGAt                                         <  5:250737/149‑1 (MQ=255)
                                                                                                               cGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGa                                                                      <  4:196156/112‑1 (MQ=255)
                                                                                                               cGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGa                                                                      >  3:196156/1‑112 (MQ=255)
                                                                                                                  gcgcGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCggg                              >  1:116660/1‑149 (MQ=255)
                                                                                                                  ccgcgGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCggg                              <  2:197689/148‑1 (MQ=255)
                                                                                                                                     cagatcaccaATGCCCCGGCATTGATGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATc            >  2:151495/5‑148 (MQ=255)
                                                                                                                                              aaTGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTcgcgc  <  2:63228/149‑1 (MQ=255)
                                                                                                                                              aaTGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGACTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTcgcgc  >  6:70559/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
AAAGGTGTTCAGCCTAGTTCAGGCCATTGGCCATTCGGGTGAAGCCATTTGGGAGCGGCTGGGGCATGAGCCTTCGGGGCAGCCGTTCAACTCGTTGGTGCAGCTGGAGTTCGAGCGCGGGCGGCCGCGTAACCCGTTCATCAATGCCGGGGCATTGGTGATCTGCGATATCAATCAGTCGCGCTTCGCGGCCCCCACGTTGTCGATGCGCGATTTTGTCCGACGGCTTTCAGGCAACCCTCACATCTCGATCAATACCCGGGTCGCTGATTCGGAATATCAGTTTCGCGC  >  NC_002947/2868728‑2869018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑