Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,876,231 T→C 8.1% T44A (ACC→GCC)  PP_2530 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,876,2310TC 8.1% 134.7 / 2.4 49T44A (ACC→GCC) PP_2530hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (21/24);  new base C (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

GCCTTGTGGAGCGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGCCCGAACCGCGCGTGGCACAGCGCCG  >  NC_002947/2876088‑2876367
                                                                                                                                               |                                                                                                                                        
gCCTTGTGGAGCGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtg                                                                                                                                      <  4:172274/148‑1 (MQ=255)
 ccTTGTGGAGCGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGGGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtggt                                                                                                                                    <  8:211417/149‑1 (MQ=255)
  cTTGTGGAGCGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtggtg                                                                                                                                   <  7:119635/149‑1 (MQ=255)
     gtgGAGCGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGTGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGGGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtggtgg                                                                                                                                  <  8:317091/147‑1 (MQ=255)
     gtgGAGCGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGc                                                                                                                                 <  3:305366/148‑1 (MQ=255)
           cGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAgg                                                                                                                          <  6:28222/149‑1 (MQ=255)
           cGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAgg                                                                                                                          <  4:182922/149‑1 (MQ=255)
                 aaTGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtgg                                                                                                                                     <  6:182860/132‑1 (MQ=255)
                 aaTGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtgg                                                                                                                                     >  5:182860/1‑132 (MQ=255)
                        tCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCcgcg                                                                                                             >  4:104090/1‑149 (MQ=255)
                                ccTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCt                                                                                                      >  3:79999/1‑148 (MQ=255)
                                   tttGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGAcc                                                                                                  >  6:264629/1‑149 (MQ=255)
                                       cgcgcAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCcgc                                                                                              >  5:333143/1‑149 (MQ=255)
                                         cgcAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGa                                                                                            <  4:79999/149‑1 (MQ=255)
                                          gcAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtggtg                                                                                                                                   <  4:275751/109‑1 (MQ=255)
                                          gcAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTtggtg                                                                                                                                   >  3:275751/1‑109 (MQ=255)
                                            aGCCGTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATg                                                                                          >  2:151270/1‑148 (MQ=255)
                                             gCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGa                                                                                        <  5:264629/149‑1 (MQ=255)
                                               cTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGAcc                                                                                      >  3:4397/1‑149 (MQ=255)
                                                   gtggCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACcgcg                                                                                   <  6:333143/148‑1 (MQ=255)
                                                         gcgcTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCtctc                                                                            <  1:151270/149‑1 (MQ=255)
                                                               gTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGcc                                                                        <  3:239777/147‑1 (MQ=255)
                                                                 aTCCGCCTTGACGGGTTGCTGGATGTTAATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCTCGCCCTCTCGGCCGGGt                                                                    >  7:147705/1‑149 (MQ=255)
                                                                   gcgcgTTGTAGGTTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGGCATCAGTGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGtt                                                                   <  8:147705/147‑1 (MQ=255)
                                                                           tCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGCTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGcgcg                                                          >  8:132990/1‑149 (MQ=255)
                                                                           tCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGCTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGcgcg                                                          >  8:128117/1‑149 (MQ=255)
                                                                              ggTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCCCCCTCTCGGCCGGGTTGCAAGCGCGTAc                                                       <  1:201603/149‑1 (MQ=255)
                                                                                   cTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCa                                                                                                                            >  8:152804/1‑75 (MQ=255)
                                                                                   cTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCa                                                                                                                            <  7:152804/75‑1 (MQ=255)
                                                                                   cTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCgg                                                   >  5:85945/1‑148 (MQ=255)
                                                                                    tGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCa                                                 >  7:158361/1‑149 (MQ=255)
                                                                                        tGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACggg                                             >  5:154971/1‑149 (MQ=255)
                                                                                          ttCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGgc                                            <  3:104090/148‑1 (MQ=255)
                                                                                           tCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCACGCGCGTACCCGGCACGGgcg                                           >  5:108918/1‑148 (MQ=255)
                                                                                             aTGCCGGCGGGAGGTGGCGGTGGGCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCAt                                        <  6:62961/149‑1 (MQ=255)
                                                                                               gccgccGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATg                                       >  6:360907/1‑148 (MQ=255)
                                                                                                 cgccgGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGcac                                    <  7:59996/149‑1 (MQ=255)
                                                                                                          gTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCTGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGc                           >  5:367717/1‑149 (MQ=255)
                                                                                                           tGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCTGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGcc                          <  6:367717/149‑1 (MQ=255)
                                                                                                             gCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGccc                         >  3:28695/1‑148 (MQ=255)
                                                                                                                   gtCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGCCCGAACcgc                  >  7:274133/1‑149 (MQ=255)
                                                                                                                                  gCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGCCCGAACCGCGCGTGGCACAGCGcc   <  4:4397/149‑1 (MQ=255)
                                                                                                                                   ccACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGtt                                                                   >  7:152132/1‑84 (MQ=255)
                                                                                                                                   ccACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGtt                                                                   <  8:152132/84‑1 (MQ=255)
                                                                                                                                   ccACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGCCCGAACCGCGCGTGGCACAGCGCCg  >  5:245571/1‑149 (MQ=255)
                                                                                                                                   ccACGGCGGTGGCTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGCCCGAACCGCGCGTGGCACAGCGCCg  <  7:128117/149‑1 (MQ=255)
                                                                                                                                   ccACGGCGGTGGCTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGCCCGAACCGCGCGTGGCACAGCGCCg  <  7:132990/149‑1 (MQ=255)
                                                                                                                                     aCGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGcaca                                   <  5:315054/114‑1 (MQ=255)
                                                                                                                                     aCGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGcaca                                   >  6:315054/1‑114 (MQ=255)
                                                                                                                                               |                                                                                                                                        
GCCTTGTGGAGCGTTTCAATGAAGTCGAAAAACCTTTTGCGCGCAGCCTTGGTGGCGGCGCTGGTATCCGCCTTGTCGGGTTGCTGGATGTTCATGCCGCCGGGAGGTGGCGGTGGTCATGGTGGGGGTGGCCACGGCGGTGGTTTTGGTGGCCATCAGGGTGGCGGCCCGCGCTAAGCTGACCCCGCGATGGACCGCGCCCTCTCGGCCGGGTTGCAAGCGCGTACCCGGCACGGGCGCATGCACATTGGTCGCCCGAACCGCGCGTGGCACAGCGCCG  >  NC_002947/2876088‑2876367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑