Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,878,895 C→T 9.7% T37T (ACG→ACA)  PP_2533 ← D‑isomer specific 2‑hydroxyacid dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,878,8950CT 9.7% 112.6 / 3.0 43T37T (ACG→ACA) PP_2533D‑isomer specific 2‑hydroxyacid dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base C (20/18);  new base T (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CCAGGTCAAGGTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCGGGAATGACAATGCGCATGGTGGTCTGGCCCGGGCGGTACAGT  >  NC_002947/2878747‑2879030
                                                                                                                                                    |                                                                                                                                       
ccAGGTCAAGGTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGc                                                                                                                                         <  6:148374/149‑1 (MQ=255)
   ggTCAAGGTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTg                                                                                                                                      <  5:279236/149‑1 (MQ=255)
    gTCAAGGTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTgg                                                                                                                                     >  4:238525/1‑149 (MQ=255)
     tCAAGGTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTgg                                                                                                                                     >  4:251570/1‑148 (MQ=255)
      cAAGGTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTgg                                                                                                                                     >  3:213024/1‑147 (MQ=255)
         ggTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGc                                                                                                                                >  6:187189/1‑149 (MQ=255)
           tGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCgg                                                                                                                              <  4:213024/149‑1 (MQ=255)
             gCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCATGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGa                                                                                                                            <  1:192064/149‑1 (MQ=255)
               tGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGTGTGGCAGGCgg                                                                                                                              >  5:222980/1‑145 (MQ=255)
               tGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGTGTGGCAGGCgg                                                                                                                              <  6:228048/145‑1 (MQ=255)
               tGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGTGTGGCAGGCgg                                                                                                                              <  6:222980/145‑1 (MQ=255)
               tGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGTGTGGCAGGCgg                                                                                                                              >  5:228048/1‑145 (MQ=255)
                 gACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCt                                                                                                                         >  4:335948/1‑148 (MQ=255)
                 gACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTc                                                                                                                        <  5:187189/149‑1 (MQ=255)
                  acacCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGt                                                                                                                                       >  7:213762/1‑133 (MQ=255)
                  acacCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGt                                                                                                                                       <  8:213762/133‑1 (MQ=255)
                      cTTGGCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCATCGATGCGTGTGCGTTCGGGGGTGAGTACCAGGGCATCGTCATCGGCAAAACTTGCGTCAAGTTATTCCAGCGTGGCAGGCGGGAGCTCATGaa                                                                                                                   <  8:288664/149‑1 (MQ=255)
                          ccGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGGCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGa                                                                                                                <  4:63780/148‑1 (MQ=255)
                           cGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACg                                                                                                              >  2:14159/1‑149 (MQ=255)
                           cGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACg                                                                                                              >  1:258641/1‑149 (MQ=255)
                                  gCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAg                                                                                                                           <  1:162353/129‑1 (MQ=255)
                                  gCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAg                                                                                                                           >  2:162353/1‑129 (MQ=255)
                                  gCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACa                                                                                                        <  3:238525/148‑1 (MQ=255)
                                  gCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAg                                                                                                       >  6:304404/1‑149 (MQ=255)
                                     gATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCAt                                                                                                    >  3:158522/1‑149 (MQ=255)
                                         aGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGccc                                                                                                <  5:304404/149‑1 (MQ=255)
                                                        aGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCg                                                                                   >  8:50669/1‑147 (MQ=255)
                                                                        cggcgTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACgg                                                                 <  6:43206/149‑1 (MQ=255)
                                                                            gTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGAAGGCGGTAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTa                                                             >  4:66401/1‑149 (MQ=255)
                                                                                    gtgtGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGAATGCCCGCTCAGCTTGCCGAACCAATCGCGGGTACGGATTACAGTTTgg                                                     >  6:346684/1‑149 (MQ=255)
                                                                                       tgCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTa                                                   <  1:14159/148‑1 (MQ=255)
                                                                                          gTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAg                                                                                                                           >  5:346974/1‑73 (MQ=255)
                                                                                          gTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAg                                                                                                                           <  6:346974/73‑1 (MQ=255)
                                                                                              gcgGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGtcgtcg                                            <  2:258641/148‑1 (MQ=255)
                                                                                              gcgGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCgg                                           <  3:335948/149‑1 (MQ=255)
                                                                                               cgGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCgg                                           >  1:22054/1‑148 (MQ=255)
                                                                                                gggTGAGTAGCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTGCAGGGTGGCAGTCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCACTCGAGGGTACGGATTAGATTTTGGTAGTCGTCggg                                          >  8:252493/1‑148 (MQ=255)
                                                                                                gggTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGaa                                                                                 <  2:329791/109‑1 (MQ=255)
                                                                                                gggTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGaa                                                                                 >  1:329791/1‑109 (MQ=255)
                                                                                                  gTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCGGGAAt                                       <  7:50669/149‑1 (MQ=255)
                                                                                                        aCCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCACTCGAGGGTACGGATTACATTTTGGTAGTCGTCGGGAATGACAAt                                 >  8:180956/1‑149 (MQ=255)
                                                                                                         ccAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATCCCCGCTCAGCTTGACGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCGGGAATGACAATg                                >  7:115513/1‑149 (MQ=255)
                                                                                                          cAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCGGGAATGACAATgc                               <  4:106321/149‑1 (MQ=255)
                                                                                                                                       cAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCGGGAATGACAATGCGCATGGTGGTCTGGCCCGGGCGGTACAGt  >  8:23525/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                       
CCAGGTCAAGGTGGCTGGACACCTTGCCGGTCTGGCTGATCAGCTTGAGGTTGGGCAGGCGGTCGAGCAAGGCGGCGTCGATGCGTGTGCGTTCGCGGGTGAGTACCAGGGCATCGGCATCGGCAAAACTTGCGGCAAGTTGTTCCAGCGTGGCAGGCGGGAGCTCATGAAGGACGCTGACAGCATGCCCGCTCAGCTTGCCGAAGCAATCGAGGGTACGGATTACATTTTGGTAGTCGTCGGGAATGACAATGCGCATGGTGGTCTGGCCCGGGCGGTACAGT  >  NC_002947/2878747‑2879030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑