Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,880,316 T→C 11.5% V53A (GTG→GCG)  PP_2535 → transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,880,3160TC11.5% 129.5 / 8.0 52V53A (GTG→GCG) PP_2535transport protein
Reads supporting (aligned to +/- strand):  ref base T (21/25);  new base C (2/4);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 6.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

AATCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGC  >  NC_002947/2880168‑2880458
                                                                                                                                                    |                                                                                                                                              
aaTCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGt                                                                                                                                                >  8:26667/1‑149 (MQ=255)
  tCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGc                                                                                                                                              >  7:291817/1‑149 (MQ=255)
  tCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGAGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTg                                                                                                                                               >  1:314149/1‑148 (MQ=255)
     aCCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGt                                                                                                                                                <  4:224105/144‑1 (MQ=255)
     aCCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGt                                                                                                                                                >  3:224105/1‑144 (MQ=255)
        gCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCg                                                                                                                                        >  1:261480/1‑149 (MQ=255)
                cACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGcgccg                                                                                                                                >  8:187585/1‑149 (MQ=255)
                     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTCTCGCTACCGACCTCGGTGTTTCCATTCCTTCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                             <  7:188248/147‑1 (MQ=255)
                     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtgat                                                                                                                           <  3:337057/149‑1 (MQ=255)
                     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtgat                                                                                                                           <  3:342572/149‑1 (MQ=255)
                     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtga                                                                                                                            <  7:189969/148‑1 (MQ=255)
                     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtg                                                                                                                             <  2:187697/147‑1 (MQ=255)
                     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                             <  2:38792/147‑1 (MQ=255)
                     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                             <  4:232128/147‑1 (MQ=255)
                                 ttCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCAc                                                                                                               >  3:147456/1‑149 (MQ=255)
                                   cGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACg                                                                                                              <  7:26667/148‑1 (MQ=255)
                                    ggCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGt                                                                                                            <  8:291817/149‑1 (MQ=255)
                                      cATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGa                                                                                                          <  2:314149/149‑1 (MQ=255)
                                           gCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATc                                                                                                     >  6:361612/1‑149 (MQ=255)
                                           gCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATc                                                                                                     <  2:261480/149‑1 (MQ=255)
                                                  agagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCgcgc                                                                                               <  1:181845/148‑1 (MQ=255)
                                                  agagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCgcgc                                                                                               >  2:181845/1‑148 (MQ=255)
                                                        tGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTAc                                                                                         <  6:3304/148‑1 (MQ=255)
                                                           ccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCTGTTACCTGt                                                                                     <  5:361612/149‑1 (MQ=255)
                                                                   gCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                             <  4:147456/149‑1 (MQ=255)
                                                                                      tACCGACCTCGGTGTTTCCATTCTTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCt                                                          >  2:89944/1‑149 (MQ=255)
                                                                                          gACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcg                                                                                                                                     <  6:140127/70‑1 (MQ=255)
                                                                                          gACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcg                                                                                                                                     >  5:140127/1‑70 (MQ=255)
                                                                                                     ttCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGt                                           >  5:204762/1‑149 (MQ=255)
                                                                                                       ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                            >  3:322672/1‑114 (MQ=255)
                                                                                                       ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                            <  4:322672/114‑1 (MQ=255)
                                                                                                         aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                       <  5:265406/133‑1 (MQ=255)
                                                                                                         aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                       >  6:265406/1‑133 (MQ=255)
                                                                                                             ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                   >  7:57511/1‑149 (MQ=255)
                                                                                                             ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                   <  8:165404/149‑1 (MQ=255)
                                                                                                              tgctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATg                                            >  8:156405/1‑139 (MQ=255)
                                                                                                              tgctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATg                                            <  7:156405/139‑1 (MQ=255)
                                                                                                                ctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                 >  7:189958/1‑148 (MQ=255)
                                                                                                                   cGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGc                             >  4:294597/1‑149 (MQ=255)
                                                                                                                     gggCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCGATTTTCACCTTGGGCAAGCTTATGTCGGCCCTGGCCAGCGa                           <  8:189958/149‑1 (MQ=255)
                                                                                                                           ctgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                     <  7:187585/149‑1 (MQ=255)
                                                                                                                           ctgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                     <  1:89944/149‑1 (MQ=255)
                                                                                                                            tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                        <  2:145528/113‑1 (MQ=255)
                                                                                                                            tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                        >  1:145528/1‑113 (MQ=255)
                                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                       <  7:95139/112‑1 (MQ=255)
                                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                       >  8:95139/1‑112 (MQ=255)
                                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                       >  2:205166/1‑112 (MQ=255)
                                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                       <  1:205166/112‑1 (MQ=255)
                                                                                                                                      cTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTTGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtgg          >  6:287980/1‑149 (MQ=255)
                                                                                                                                        aTGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                 <  6:191917/124‑1 (MQ=255)
                                                                                                                                        aTGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                 >  5:191917/1‑124 (MQ=255)
                                                                                                                                         tGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtg        <  8:57511/148‑1 (MQ=255)
                                                                                                                                              gcGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCAGTTTCACCTTCGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCcgc  >  5:215473/2‑149 (MQ=255)
                                                                                                                                               cGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGc                 >  4:279822/1‑133 (MQ=255)
                                                                                                                                               cGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGc                 <  3:279822/133‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
AATCCACCGCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGC  >  NC_002947/2880168‑2880458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑