Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,880,324 G→A 11.5% G56S (GGC→AGC)  PP_2535 → transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,880,3240GA11.5% 138.5 / 6.2 52G56S (GGC→AGC) PP_2535transport protein
Reads supporting (aligned to +/- strand):  ref base G (20/26);  new base A (2/4);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

GCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCAC  >  NC_002947/2880176‑2880471
                                                                                                                                                    |                                                                                                                                                   
gCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCg                                                                                                                                                     >  1:261480/1‑149 (MQ=255)
        cACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGcgccg                                                                                                                                             >  8:187585/1‑149 (MQ=255)
             gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTCTCGCTACCGACCTCGGTGTTTCCATTCCTTCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                                          <  7:188248/147‑1 (MQ=255)
             gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtgat                                                                                                                                        <  3:342572/149‑1 (MQ=255)
             gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtgat                                                                                                                                        <  3:337057/149‑1 (MQ=255)
             gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtga                                                                                                                                         <  7:189969/148‑1 (MQ=255)
             gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtg                                                                                                                                          <  2:187697/147‑1 (MQ=255)
             gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                                          <  4:232128/147‑1 (MQ=255)
             gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                                          <  2:38792/147‑1 (MQ=255)
                         ttCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCAc                                                                                                                            >  3:147456/1‑149 (MQ=255)
                           cGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACg                                                                                                                           <  7:26667/148‑1 (MQ=255)
                            ggCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGt                                                                                                                         <  8:291817/149‑1 (MQ=255)
                              cATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGa                                                                                                                       <  2:314149/149‑1 (MQ=255)
                                   gCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATc                                                                                                                  >  6:361612/1‑149 (MQ=255)
                                   gCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATc                                                                                                                  <  2:261480/149‑1 (MQ=255)
                                          agagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCgcgc                                                                                                            >  2:181845/1‑148 (MQ=255)
                                          agagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCgcgc                                                                                                            <  1:181845/148‑1 (MQ=255)
                                                tGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTAc                                                                                                      <  6:3304/148‑1 (MQ=255)
                                                   ccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCTGTTACCTGt                                                                                                  <  5:361612/149‑1 (MQ=255)
                                                           gCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                                          <  4:147456/149‑1 (MQ=255)
                                                                              tACCGACCTCGGTGTTTCCATTCTTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCt                                                                       >  2:89944/1‑149 (MQ=255)
                                                                                  gACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcg                                                                                                                                                  <  6:140127/70‑1 (MQ=255)
                                                                                  gACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcg                                                                                                                                                  >  5:140127/1‑70 (MQ=255)
                                                                                             ttCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGt                                                        >  5:204762/1‑149 (MQ=255)
                                                                                               ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                                         <  4:322672/114‑1 (MQ=255)
                                                                                               ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                                         >  3:322672/1‑114 (MQ=255)
                                                                                                 aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                    <  5:265406/133‑1 (MQ=255)
                                                                                                 aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                    >  6:265406/1‑133 (MQ=255)
                                                                                                     ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                                <  8:165404/149‑1 (MQ=255)
                                                                                                     ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                                >  7:57511/1‑149 (MQ=255)
                                                                                                      tgctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATg                                                         >  8:156405/1‑139 (MQ=255)
                                                                                                      tgctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATg                                                         <  7:156405/139‑1 (MQ=255)
                                                                                                        ctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                              >  7:189958/1‑148 (MQ=255)
                                                                                                           cGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGc                                          >  4:294597/1‑149 (MQ=255)
                                                                                                             gggCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCGATTTTCACCTTGGGCAAGCTTATGTCGGCCCTGGCCAGCGa                                        <  8:189958/149‑1 (MQ=255)
                                                                                                                   ctgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                                  <  7:187585/149‑1 (MQ=255)
                                                                                                                   ctgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                                  <  1:89944/149‑1 (MQ=255)
                                                                                                                    tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                                     >  1:145528/1‑113 (MQ=255)
                                                                                                                    tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                                     <  2:145528/113‑1 (MQ=255)
                                                                                                                      gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                    <  7:95139/112‑1 (MQ=255)
                                                                                                                      gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                    >  8:95139/1‑112 (MQ=255)
                                                                                                                      gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                    <  1:205166/112‑1 (MQ=255)
                                                                                                                      gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                    >  2:205166/1‑112 (MQ=255)
                                                                                                                              cTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTTGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtgg                       >  6:287980/1‑149 (MQ=255)
                                                                                                                                aTGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                              <  6:191917/124‑1 (MQ=255)
                                                                                                                                aTGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                              >  5:191917/1‑124 (MQ=255)
                                                                                                                                 tGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtg                     <  8:57511/148‑1 (MQ=255)
                                                                                                                                      gcGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCAGTTTCACCTTCGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCcgc               >  5:215473/2‑149 (MQ=255)
                                                                                                                                       cGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGc                              >  4:279822/1‑133 (MQ=255)
                                                                                                                                       cGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGc                              <  3:279822/133‑1 (MQ=255)
                                                                                                                                              cTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCcgcg              >  3:90470/1‑142 (MQ=255)
                                                                                                                                              cTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCcgcg              <  4:90470/142‑1 (MQ=255)
                                                                                                                                                 aTCGGCGCGCCGCTGATGACCCTGGCCACCGTGAAGATCCAGCGCAGTTACCTGTTGATCGGGCTGATGGACATTATCACATTAGGCAACATGATGTCGGCCCTGGCCAGCGACTACGACAGCATGCTGGTGGCCCGCGTGGtaactt     >  8:131842/1‑148 (MQ=255)
                                                                                                                                                    ggCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGtcacttcac  <  6:204762/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCTTGTCGCACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCAC  >  NC_002947/2880176‑2880471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑