Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,880,327 G→A 11.7% A57T (GCG→ACG)  PP_2535 → transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,880,3270GA11.7% 134.7 / 6.7 51A57T (GCG→ACG) PP_2535transport protein
Reads supporting (aligned to +/- strand):  ref base G (19/26);  new base A (2/4);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACT  >  NC_002947/2880184‑2880472
                                                                                                                                               |                                                                                                                                                 
cACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGcgccg                                                                                                                                              >  8:187585/1‑149 (MQ=255)
     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTCTCGCTACCGACCTCGGTGTTTCCATTCCTTCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                                           <  7:188248/147‑1 (MQ=255)
     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtgat                                                                                                                                         <  3:337057/149‑1 (MQ=255)
     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtgat                                                                                                                                         <  3:342572/149‑1 (MQ=255)
     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtga                                                                                                                                          <  7:189969/148‑1 (MQ=255)
     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCtg                                                                                                                                           <  2:187697/147‑1 (MQ=255)
     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                                           <  4:232128/147‑1 (MQ=255)
     gCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGCGCCGATCAGCACGCCGAtg                                                                                                                                           <  2:38792/147‑1 (MQ=255)
                 ttCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCAc                                                                                                                             >  3:147456/1‑149 (MQ=255)
                   cGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACg                                                                                                                            <  7:26667/148‑1 (MQ=255)
                    ggCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGt                                                                                                                          <  8:291817/149‑1 (MQ=255)
                      cATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGa                                                                                                                        <  2:314149/149‑1 (MQ=255)
                           gCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATc                                                                                                                   >  6:361612/1‑149 (MQ=255)
                           gCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATc                                                                                                                   <  2:261480/149‑1 (MQ=255)
                                  agagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCgcgc                                                                                                             >  2:181845/1‑148 (MQ=255)
                                  agagTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCgcgc                                                                                                             <  1:181845/148‑1 (MQ=255)
                                        tGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTAc                                                                                                       <  6:3304/148‑1 (MQ=255)
                                           ccccATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCTGTTACCTGt                                                                                                   <  5:361612/149‑1 (MQ=255)
                                                   gCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCggg                                                                                           <  4:147456/149‑1 (MQ=255)
                                                                      tACCGACCTCGGTGTTTCCATTCTTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCt                                                                        >  2:89944/1‑149 (MQ=255)
                                                                          gACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcg                                                                                                                                                   <  6:140127/70‑1 (MQ=255)
                                                                          gACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGgcg                                                                                                                                                   >  5:140127/1‑70 (MQ=255)
                                                                                     ttCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGt                                                         >  5:204762/1‑149 (MQ=255)
                                                                                       ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                                          >  3:322672/1‑114 (MQ=255)
                                                                                       ccATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGc                                                                                          <  4:322672/114‑1 (MQ=255)
                                                                                         aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                     >  6:265406/1‑133 (MQ=255)
                                                                                         aTTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                     <  5:265406/133‑1 (MQ=255)
                                                                                             ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                                 >  7:57511/1‑149 (MQ=255)
                                                                                             ctgctgCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTg                                                 <  8:165404/149‑1 (MQ=255)
                                                                                              tgctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATg                                                          >  8:156405/1‑139 (MQ=255)
                                                                                              tgctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATg                                                          <  7:156405/139‑1 (MQ=255)
                                                                                                ctgcGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                               >  7:189958/1‑148 (MQ=255)
                                                                                                   cGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGc                                           >  4:294597/1‑149 (MQ=255)
                                                                                                     gggCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCGATTTTCACCTTGGGCAAGCTTATGTCGGCCCTGGCCAGCGa                                         <  8:189958/149‑1 (MQ=255)
                                                                                                           ctgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                                   <  7:187585/149‑1 (MQ=255)
                                                                                                           ctgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGc                                   <  1:89944/149‑1 (MQ=255)
                                                                                                            tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                                      >  1:145528/1‑113 (MQ=255)
                                                                                                            tgGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTg                                                                      <  2:145528/113‑1 (MQ=255)
                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                     <  7:95139/112‑1 (MQ=255)
                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                     >  8:95139/1‑112 (MQ=255)
                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                     >  2:205166/1‑112 (MQ=255)
                                                                                                              gTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTgg                                                                     <  1:205166/112‑1 (MQ=255)
                                                                                                                      cTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTTGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtgg                        >  6:287980/1‑149 (MQ=255)
                                                                                                                        aTGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                               <  6:191917/124‑1 (MQ=255)
                                                                                                                        aTGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGc                                               >  5:191917/1‑124 (MQ=255)
                                                                                                                         tGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCtggtg                      <  8:57511/148‑1 (MQ=255)
                                                                                                                              gcGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCAGTTTCACCTTCGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCcgc                >  5:215473/2‑149 (MQ=255)
                                                                                                                               cGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGc                               >  4:279822/1‑133 (MQ=255)
                                                                                                                               cGGCGCGCCGATCAGCACGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGc                               <  3:279822/133‑1 (MQ=255)
                                                                                                                                      cTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCcgcg               <  4:90470/142‑1 (MQ=255)
                                                                                                                                      cTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCcgcg               >  3:90470/1‑142 (MQ=255)
                                                                                                                                         aTCGGCGCGCCGCTGATGACCCTGGCCACCGTGAAGATCCAGCGCAGTTACCTGTTGATCGGGCTGATGGACATTATCACATTAGGCAACATGATGTCGGCCCTGGCCAGCGACTACGACAGCATGCTGGTGGCCCGCGTGGtaactt      >  8:131842/1‑148 (MQ=255)
                                                                                                                                            ggCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGtcacttcac   <  6:204762/148‑1 (MQ=255)
                                                                                                                                             gcgcgcCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGcc                                  >  2:94722/1‑116 (MQ=255)
                                                                                                                                             gcgcgcCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGcc                                  <  1:94722/116‑1 (MQ=255)
                                                                                                                                             gcgcgcCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGtcacttcact  >  1:163748/1‑148 (MQ=255)
                                                                                                                                               gcgcCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAg                                >  1:335865/1‑116 (MQ=255)
                                                                                                                                               gcgcCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAg                                <  2:335865/116‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CACTCGCCATCGGTGCCTTCGGCATCGGCGTTACAGAGTTTGCCCCCATGGGCATGTTGCCCAGTATCGCTACCGACCTCGGTGTTTCCATTCCTGCTGCGGGGCTGCTGGTCAGTGCCTATGCCATCGGCGTGCTGATCGGCGCGCCGCTGATGACCCTGGCCACGGTGAAGATCCCGCGCCGTTACCTGTTGATCGGGCTGATGGCCATTTTCACCTTGGGCAACCTGATGTCGGCCCTGGCCAGCGACTACGCCAGCCTGCTGGTGGCCCGCGTGGTCACTTCACT  >  NC_002947/2880184‑2880472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑