Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,885,364 C→G 7.4% S172T (AGC→ACC)  PP_2541 ← transcriptional factor‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,885,3640CG 7.4% 164.0 / 4.1 54S172T (AGC→ACC) PP_2541transcriptional factor‑like protein
Reads supporting (aligned to +/- strand):  ref base C (29/21);  new base G (2/2);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

GCGCGTATTGCGCATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGCGCCGCCACTAGCCTGCGGCGT  >  NC_002947/2885218‑2885512
                                                                                                                                                  |                                                                                                                                                    
gcgcGTATTGCGCATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGct                                                                                                                                                     <  2:173382/148‑1 (MQ=255)
  gcgTATTGCGCATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGCCAGAACCTGCTGATCGATGGCGGGGCCTACCCCCGAACCTACTGACGCCGTTGGCCCTATTGCTTGCAGTCGCGTTCGCCCTCATGCACCGCCACGCACTg                                                                                                                                                  >  3:42762/1‑149 (MQ=255)
       ttGCGCATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGgcgc                                                                                                                                              >  2:50695/1‑148 (MQ=255)
        tGCGCATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCaa                                                                                                                                            >  7:51521/1‑149 (MQ=255)
         gcgcATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTTCTGATCGATGGCCGGGCGGACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCaa                                                                                                                                            <  5:370058/148‑1 (MQ=255)
         gcgcATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAAt                                                                                                                                           >  3:84114/1‑149 (MQ=255)
          cgcATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAAtt                                                                                                                                          <  1:50695/149‑1 (MQ=255)
                      cAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATg                                                                                                                               <  5:309566/148‑1 (MQ=255)
                          gcgcATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCcgc                                                                                                                          <  5:166759/149‑1 (MQ=255)
                           cgcATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGTGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCcgcg                                                                                                                         >  2:245750/1‑149 (MQ=255)
                                gCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAg                                                                                                                    >  2:179050/1‑149 (MQ=255)
                                        cATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTgcg                                                                                                            >  6:257036/1‑149 (MQ=255)
                                        cATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTgc                                                                                                             <  5:57456/148‑1 (MQ=255)
                                             ccGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGcc                                                                                                       >  4:298913/1‑149 (MQ=255)
                                              cGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCt                                                                                                      >  6:78505/1‑149 (MQ=255)
                                                  cAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGAt                                                                                                  >  2:40003/1‑149 (MQ=255)
                                                       ccTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGAGCCTGgcgct                                                                                                                                             >  2:99683/1‑100 (MQ=255)
                                                       ccTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGCTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGAGCCTGgcgct                                                                                                                                             <  1:99683/101‑2 (MQ=255)
                                                                     gCGGGGCATACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTgg                                                                               <  8:214423/149‑1 (MQ=255)
                                                                           cGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGAcagc                                                                         <  3:298913/149‑1 (MQ=255)
                                                                             tACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATc                                                                                           >  2:216789/1‑129 (MQ=255)
                                                                             tACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATc                                                                                           <  1:216789/129‑1 (MQ=255)
                                                                               ccccGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACagcagc                                                                      <  5:238828/148‑1 (MQ=255)
                                                                                 ccGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGA‑CCCGCCTgg                                                                               >  8:326136/1‑136 (MQ=255)
                                                                                 ccGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGA‑CCCGCCTgg                                                                               <  7:326136/136‑1 (MQ=255)
                                                                                  cGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGcagccagc                                                                  <  4:84114/149‑1 (MQ=255)
                                                                                         tACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCTCCTGCGGCGCCTGGATGCGGATCGACCCAGCCTGGGACAGCAGCCAGCCCATCAg                                                           >  8:44309/1‑149 (MQ=255)
                                                                                         tACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAg                                                           >  8:49911/1‑149 (MQ=255)
                                                                                         tACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCAGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAg                                                           >  1:294939/1‑149 (MQ=255)
                                                                                          aCTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGCCGCCTGGATGCGGATCGACCACGCCTGGGACAGCAGCCCGCCCATCAgc                                                          >  6:39775/1‑149 (MQ=255)
                                                                                           cTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAgc                                                          <  5:257036/148‑1 (MQ=255)
                                                                                              aCGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGTATGCGGATCGACCCCGCCTGGGAca                                                                           <  5:206606/128‑1 (MQ=255)
                                                                                              aCGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGAca                                                                           >  6:206606/1‑128 (MQ=255)
                                                                                              aCGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAgcgcgc                                                      >  4:146972/1‑149 (MQ=255)
                                                                                               cGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGcgcgcg                                                     <  2:294939/149‑1 (MQ=255)
                                                                                                  cGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGtt                                                  >  7:142070/1‑149 (MQ=255)
                                                                                                   gTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTg                                                 <  1:245750/149‑1 (MQ=255)
                                                                                                      ggCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTgtc                                               <  5:78505/148‑1 (MQ=255)
                                                                                                       gcgcTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTgtcgt                                             >  1:204564/1‑149 (MQ=255)
                                                                                                          cTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGa                                          >  6:347730/1‑149 (MQ=255)
                                                                                                               gcttgcAGTCGCCTTCGCCCGGATGCAGGTGCAGGCGCTGGCGCAATTGTTCTAGCATGGCGGCGCGCAGCGCCTGCTGCGCCTGCATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCGATCACCGCGCGGTTGTGGTCGACGGt                                      <  4:42762/148‑1 (MQ=255)
                                                                                                                cttgcAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTgg                                    >  5:317774/1‑149 (MQ=255)
                                                                                                                     aGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGccacc                               >  3:77602/1‑149 (MQ=255)
                                                                                                                           cTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGcgcg                         >  1:138414/1‑149 (MQ=255)
                                                                                                                               gCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGgcgc                     >  3:106639/1‑149 (MQ=255)
                                                                                                                               gCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTCGATGCGGATCCACCCCGCCTGGGACAGCAGCCAGACCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGgcgc                     >  7:317408/1‑149 (MQ=255)
                                                                                                                                cccTCATGCAGCGCCAGGCTCTAGCGCAATTGTTCAATCATGGCCGAGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGcgcc                    >  4:149971/1‑149 (MQ=255)
                                                                                                                                  cTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGcgccgc                  <  6:317774/149‑1 (MQ=255)
                                                                                                                                   tCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGcgccgc                  >  3:4498/1‑148 (MQ=255)
                                                                                                                                       gCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGCGCCGCCAc               >  6:16454/1‑147 (MQ=255)
                                                                                                                                        ttgcgcCAGAGCCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAgc                            >  6:216021/3‑133 (MQ=255)
                                                                                                                                        ttgcgcCAGAGCCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAgc                            <  5:216021/131‑1 (MQ=255)
                                                                                                                                               aGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGCGCCGCCACTAGCCTgcgg     <  5:347730/149‑1 (MQ=255)
                                                                                                                                               aGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGCGCCGCCACTAGCCTgcgg     <  4:4498/149‑1 (MQ=255)
                                                                                                                                                ggCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGCGCCGCCACTAGCCTgcggc    <  1:40003/149‑1 (MQ=255)
                                                                                                                                                  ctctGGCGCAATTGTTCAATCATGGCCGCGCGCCGCGCCTGCGGCGCCTGTATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGCGCCGCCACTAGCCTGCGGCGt  <  8:51521/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GCGCGTATTGCGCATTTCTGTGCAGCGCGCATGCCGGGTACATCACCGGGCAGAACCTGCTGATCGATGGCGGGGCGTACCCCGGAACCTACTGACGCCGTTGGCGCTATTGCTTGCAGTCGCCTTCGCCCTCATGCAGCGCCAGGCTCTGGCGCAATTGTTCAATCATGGCCGCGCGCAGCGCCTGCGGCGCCTGGATGCGGATCGACCCCGCCTGGGACAGCAGCCAGCCCATCAGCGCGCGGTTGTCGTCGACGGTGGCCACCAGCGCGGCGCCGCCACTAGCCTGCGGCGT  >  NC_002947/2885218‑2885512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑