Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,891,803	A→T	9.4%	L13Q (CTG→CAG)	PP_2546 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,891,803	0	A	T	9.4%	174.7 / 5.0	61	L13Q (CTG→CAG)	PP_2546	membrane protein
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base T (3/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCAGGTGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCG  >  NC_002947/2891660‑2891945                                                                                                                                                   |                                                                                                                                               cccAGGTGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGAGGTAGAACAGGGCAGGGCAGCCACCCCAGCACCAGCCGCCCCCCGCACAGCACCCGCAGGATGAAGGCCCCGTgcacc                                                                                                                                              >  1:111700/1‑147 (MQ=255)       tggtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                     <  4:61925/149‑1 (MQ=255)         gtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGgt                                                                                                                                   <  7:115469/149‑1 (MQ=255)          tgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGgtg                                                                                                                                  >  4:125599/1‑149 (MQ=255)            aGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGTCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTc                                                                                                                                <  7:165274/149‑1 (MQ=255)            aGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTc                                                                                                                                <  8:304604/149‑1 (MQ=255)               gggCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcggc                                                                                                                             <  2:222676/149‑1 (MQ=255)                 gCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcggcgg                                                                                                                           >  4:173994/1‑149 (MQ=255)                     cccAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAg                                                                                                                       >  8:193981/1‑149 (MQ=255)                       cAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGc                                                                                                                     >  5:121287/1‑149 (MQ=255)                            gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                     <  3:98402/128‑1 (MQ=255)                            gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                     >  4:98402/1‑128 (MQ=255)                            gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                               <  8:41976/134‑1 (MQ=255)                            gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                               >  7:41976/1‑134 (MQ=255)                             cAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTaga                                                                                                               <  2:111700/149‑1 (MQ=255)                                gCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCa                                                                                                            <  6:121287/149‑1 (MQ=255)                                      cACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggtt                                                                                                      >  4:176065/1‑149 (MQ=255)                                         cGCCAGGCCCCAGGGGAGGAGCGGCTAGTGCAG‑‑CAACAGGGGAGGGC‑GCCAGCGGAGCAGCAGCAGCCCGCCGAACAGCACCAGCAGGTTGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCgg                                                                                                                   <  1:296712/133‑1 (MQ=255)                                         cGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCACCACCAGCAGCCCCCCGAACACCACCAGCACGATGAAGGCCACGTGCAGCAGCACCACGGTGTCGGCGGCAAGGCgg                                                                                                                   >  2:296712/1‑133 (MQ=255)                                                  cgatatcaggACCTGCAAGTGCAT‑‑CAACAGGGCATGGC‑GCCAGCGCATCACCATCAGCCCGCCGAACCGCACCATCAGGATTAAGGCCATGTGCATCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCggg                                                                                           <  8:312594/142‑1 (MQ=255)                                                  ccaggccaggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                               <  6:306198/112‑1 (MQ=255)                                                  ccaggccaggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                               >  5:306198/1‑112 (MQ=255)                                                  ccaggccaggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAt                                                                                                           >  2:214322/1‑132 (MQ=255)                                                  ccaggccaggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAt                                                                                                           <  1:214322/132‑1 (MQ=255)                                                  ccaggccaggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCggg                                                                                           >  2:262118/1‑148 (MQ=255)                                                      gccaggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTg                                                                                      <  1:262118/149‑1 (MQ=255)                                                        caggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                    <  7:193981/149‑1 (MQ=255)                                                         aggACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGCCGGTAGAGCATGGGTTGGTTCCCCggg                                                                                           >  7:312594/1‑141 (MQ=255)                                                                        ag‑‑caACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGt                                                                    >  4:23035/1‑149 (MQ=255)                                                                                  ggcagggc‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGttac                                                            <  3:173994/149‑3 (MQ=255)                                                                                   gcagggc‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGcc                                                           <  3:125599/149‑1 (MQ=255)                                                                                    cagggc‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCCGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCGAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCt                                                          >  3:156447/1‑149 (MQ=255)                                                                                         c‑gcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATcc                                                                             >  4:214764/1‑125 (MQ=255)                                                                                         c‑gcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATcc                                                                             <  3:214764/125‑1 (MQ=255)                                                                                              aGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGcc                                                 >  3:320349/1‑149 (MQ=255)                                                                                                     caCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTg                                                                                      >  6:84542/1‑105 (MQ=255)                                                                                                     caCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTg                                                                                      <  5:84542/105‑1 (MQ=255)                                                                                                       ccAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGcc                                                           >  8:241999/1‑130 (MQ=255)                                                                                                       ccAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGcc                                                           <  7:241999/130‑1 (MQ=255)                                                                                                        cagcagCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCtt                                       >  1:295828/1‑149 (MQ=255)                                                                                                           cagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCt                                    >  4:138573/1‑149 (MQ=255)                                                                                                            agcCCGCCGAACAGCACCAGCAGGAGGAAGGCCATGTGCAGCATCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGAGTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTc                                   <  2:22340/149‑1 (MQ=255)                                                                                                              cccGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGa                                 >  5:24222/1‑149 (MQ=255)                                                                                                              cccGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGa                                 >  1:18421/1‑149 (MQ=255)                                                                                                                      aCAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGc                         <  2:295828/149‑1 (MQ=255)                                                                                                                        aGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTac                                              >  6:92639/1‑126 (MQ=255)                                                                                                                        aGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTac                                              <  5:92639/126‑1 (MQ=255)                                                                                                                        aGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAg                       <  3:23035/149‑1 (MQ=255)                                                                                                                          cACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAcc                               >  5:157849/1‑139 (MQ=255)                                                                                                                          cACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAcc                               <  6:157849/139‑1 (MQ=255)                                                                                                                                   gATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAc                                <  4:40478/129‑1 (MQ=255)                                                                                                                                   gATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAc                                >  3:40478/1‑129 (MQ=255)                                                                                                                                    aTGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGccac           >  3:52346/1‑149 (MQ=255)                                                                                                                                          gCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTacac                                            >  2:208187/1‑110 (MQ=255)                                                                                                                                          gCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTacac                                            <  1:208187/110‑1 (MQ=255)                                                                                                                                           cctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGACTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCgg    >  8:175195/4‑149 (MQ=255)                                                                                                                                            ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                   <  2:96601/131‑1 (MQ=255)                                                                                                                                            ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                   >  7:106758/3‑133 (MQ=255)                                                                                                                                            ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                   <  8:106758/131‑1 (MQ=255)                                                                                                                                            ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                   >  1:96601/3‑133 (MQ=255)                                                                                                                                            ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGgc   <  7:175195/147‑1 (MQ=255)                                                                                                                                             aGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGgcg  <  4:320349/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               CCCAGGTGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAG‑‑CAACAGGGCAGGGC‑GCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCG  >  NC_002947/2891660‑2891945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑