Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,891,806 A→T 9.8% L12Q (CTG→CAG)  PP_2546 ← membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,891,8060AT 9.8% 166.6 / 8.1 61L12Q (CTG→CAG) PP_2546membrane protein
Reads supporting (aligned to +/- strand):  ref base A (27/28);  new base T (3/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

TGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGTACGCT  >  NC_002947/2891666‑2891953
                                                                                                                                            |                                                                                                                                                   
tggtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                             <  4:61925/149‑1 (MQ=255)
  gtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGgt                                                                                                                                           <  7:115469/149‑1 (MQ=255)
   tgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGgtg                                                                                                                                          >  4:125599/1‑149 (MQ=255)
     aGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGTCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTc                                                                                                                                        <  7:165274/149‑1 (MQ=255)
     aGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTc                                                                                                                                        <  8:304604/149‑1 (MQ=255)
        gggCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcggc                                                                                                                                     <  2:222676/149‑1 (MQ=255)
          gCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcggcgg                                                                                                                                   >  4:173994/1‑149 (MQ=255)
              cccAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAg                                                                                                                               >  8:193981/1‑149 (MQ=255)
                cAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGc                                                                                                                             >  5:121287/1‑149 (MQ=255)
                     gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                             <  3:98402/128‑1 (MQ=255)
                     gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                             >  4:98402/1‑128 (MQ=255)
                     gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                                       <  8:41976/134‑1 (MQ=255)
                     gCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                                       >  7:41976/1‑134 (MQ=255)
                      cAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTaga                                                                                                                       <  2:111700/149‑1 (MQ=255)
                         gCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCa                                                                                                                    <  6:121287/149‑1 (MQ=255)
                               cACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggtt                                                                                                              >  4:176065/1‑149 (MQ=255)
                                  cGCCAGGCCCCAGGGGAGGAGCGGCTAGTGCAGCAACAGGGGAGGGCGCCAGCGGAGCAGCAGCAGCCCGCCGAACAGCACCAGCAGGTTGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCgg                                                                                                                           <  1:296712/133‑1 (MQ=255)
                                  cGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCACCACCAGCAGCCCCCCGAACACCACCAGCACGATGAAGGCCACGTGCAGCAGCACCACGGTGTCGGCGGCAAGGCgg                                                                                                                           >  2:296712/1‑133 (MQ=255)
                                           cgatatcaggACCTGCAAGTGCATCAACAGGGCATGGCGCCAGCGCATCACCATCAGCCCGCCGAACCGCACCATCAGGATTAAGGCCATGTGCATCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCggg                                                                                                   <  8:312594/142‑1 (MQ=255)
                                           ccaggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                                       <  6:306198/112‑1 (MQ=255)
                                           ccaggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcg                                                                                                                                       >  5:306198/1‑112 (MQ=255)
                                           ccaggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAt                                                                                                                   >  2:214322/1‑132 (MQ=255)
                                           ccaggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAt                                                                                                                   <  1:214322/132‑1 (MQ=255)
                                           ccaggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCggg                                                                                                   >  2:262118/1‑148 (MQ=255)
                                               gccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTg                                                                                              <  1:262118/149‑1 (MQ=255)
                                                 caggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                            <  7:193981/149‑1 (MQ=255)
                                                  aggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGCCGGTAGAGCATGGGTTGGTTCCCCggg                                                                                                   >  7:312594/1‑141 (MQ=255)
                                                                 agcaACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGt                                                                            >  4:23035/1‑149 (MQ=255)
                                                                         ggcagggcGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGttac                                                                    <  3:173994/149‑3 (MQ=255)
                                                                          gcagggcGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGcc                                                                   <  3:125599/149‑1 (MQ=255)
                                                                           cagggcGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCCGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCGAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCt                                                                  >  3:156447/1‑149 (MQ=255)
                                                                                cgcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATcc                                                                                     >  4:214764/1‑125 (MQ=255)
                                                                                cgcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATcc                                                                                     <  3:214764/125‑1 (MQ=255)
                                                                                    aGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGcc                                                         >  3:320349/1‑149 (MQ=255)
                                                                                           caCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTg                                                                                              <  5:84542/105‑1 (MQ=255)
                                                                                           caCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTg                                                                                              >  6:84542/1‑105 (MQ=255)
                                                                                             ccAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGcc                                                                   <  7:241999/130‑1 (MQ=255)
                                                                                             ccAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGcc                                                                   >  8:241999/1‑130 (MQ=255)
                                                                                              cagcagCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCtt                                               >  1:295828/1‑149 (MQ=255)
                                                                                                 cagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCt                                            >  4:138573/1‑149 (MQ=255)
                                                                                                  agcCCGCCGAACAGCACCAGCAGGAGGAAGGCCATGTGCAGCATCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGAGTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTc                                           <  2:22340/149‑1 (MQ=255)
                                                                                                    cccGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGa                                         >  1:18421/1‑149 (MQ=255)
                                                                                                    cccGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGa                                         >  5:24222/1‑149 (MQ=255)
                                                                                                            aCAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGc                                 <  2:295828/149‑1 (MQ=255)
                                                                                                              aGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTac                                                      <  5:92639/126‑1 (MQ=255)
                                                                                                              aGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTac                                                      >  6:92639/1‑126 (MQ=255)
                                                                                                              aGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAg                               <  3:23035/149‑1 (MQ=255)
                                                                                                                cACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAcc                                       <  6:157849/139‑1 (MQ=255)
                                                                                                                cACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAcc                                       >  5:157849/1‑139 (MQ=255)
                                                                                                                         gATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAc                                        <  4:40478/129‑1 (MQ=255)
                                                                                                                         gATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAc                                        >  3:40478/1‑129 (MQ=255)
                                                                                                                          aTGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGccac                   >  3:52346/1‑149 (MQ=255)
                                                                                                                                gCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTacac                                                    <  1:208187/110‑1 (MQ=255)
                                                                                                                                gCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTacac                                                    >  2:208187/1‑110 (MQ=255)
                                                                                                                                 cctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGACTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCgg            >  8:175195/4‑149 (MQ=255)
                                                                                                                                  ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                           >  7:106758/3‑133 (MQ=255)
                                                                                                                                  ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                           <  8:106758/131‑1 (MQ=255)
                                                                                                                                  ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                           <  2:96601/131‑1 (MQ=255)
                                                                                                                                  ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                           >  1:96601/3‑133 (MQ=255)
                                                                                                                                  ctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGgc           <  7:175195/147‑1 (MQ=255)
                                                                                                                                   aGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGgcg          <  4:320349/149‑1 (MQ=255)
                                                                                                                                          gcagcaCCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGTACgc   <  3:138573/149‑1 (MQ=255)
                                                                                                                                          gcagcaCCAGGGTGTCGCCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTGTTCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCGTCCTCGTCCATCTGCAGGAGAGTCGGCACCACCCGGCGCGGACgc   >  8:10856/1‑149 (MQ=255)
                                                                                                                                           cagcaCCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGTACgct  >  4:302922/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
TGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGTACGCT  >  NC_002947/2891666‑2891953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑