Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,892,777 A→G 7.1% intergenic (‑10/‑234) PP_2547 ← / → PP_2548 LysR family transcriptional regulator/sugar MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,892,7770AG 7.1% 160.1 / 2.1 56intergenic (‑10/‑234)PP_2547/PP_2548LysR family transcriptional regulator/sugar MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base G (2/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

CGCCGAAGCGCTCCTCCAGTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGACCCGATGA  >  NC_002947/2892629‑2892921
                                                                                                                                                     |                                                                                                                                                
cGCCGAAGCGCTCCTCCAGTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCa                                                                                                                                                  <  2:296550/149‑1 (MQ=255)
 gCCGAAGCGCTCCTCCAGTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAg                                                                                                                                                 <  7:147960/149‑1 (MQ=255)
               ccAGTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCt                                                                                                                                   >  8:70322/1‑149 (MQ=255)
                cAGTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCt                                                                                                                                   >  2:170395/1‑148 (MQ=255)
                  gTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTAt                                                                                                                                <  7:70322/149‑1 (MQ=255)
                  gTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTAt                                                                                                                                <  8:50110/149‑1 (MQ=255)
                      gCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTAtgtgt                                                                                                                            >  5:29044/1‑149 (MQ=255)
                        gTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATgtgtgt                                                                                                                          <  7:214515/149‑1 (MQ=255)
                          aCCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTaa                                                                                                                        >  2:217031/1‑149 (MQ=255)
                               gTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGa                                                                                                                   >  2:26301/1‑149 (MQ=255)
                                  ggACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCGGTAACAAGCTGGGCTTATGTGTGTAATa                                                                                                                      >  1:324303/1‑143 (MQ=255)
                                  ggACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCGGTAACAAGCTGGGCTTATGTGTGTAATa                                                                                                                      >  1:329488/1‑143 (MQ=255)
                                  ggACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCGGTAACAAGCTGGGCTTATGTGTGTAATa                                                                                                                      <  2:329488/143‑1 (MQ=255)
                                  ggACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCGGTAACAAGCTGGGCTTATGTGTGTAATa                                                                                                                      <  2:324303/143‑1 (MQ=255)
                                      acCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATc                                                                                                             >  5:368073/1‑148 (MQ=255)
                                       ccGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGa                                                                                                           <  1:170395/149‑1 (MQ=255)
                                        cGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGAt                                                                                                          <  4:152621/149‑1 (MQ=255)
                                        cGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGAt                                                                                                          >  5:157445/1‑149 (MQ=255)
                                                  gTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATc                                                                                                <  3:251848/149‑1 (MQ=255)
                                                   tAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATa                                                                                                                      <  8:164179/126‑1 (MQ=255)
                                                   tAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATa                                                                                                                      >  7:164179/1‑126 (MQ=255)
                                                   tAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATtttatt                                                                                                    >  7:24266/1‑144 (MQ=255)
                                                   tAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATtttatt                                                                                                    <  8:24266/144‑1 (MQ=255)
                                                      gAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTAc                                                                                            <  1:217031/149‑1 (MQ=255)
                                                       aaaCAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCACGCGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGAt                                                                                                                  >  1:255118/1‑127 (MQ=255)
                                                       aaaCAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGAt                                                                                                                  <  2:255118/126‑1 (MQ=255)
                                                            gCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAAc                                                                                      <  8:119299/149‑1 (MQ=255)
                                                                 tCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCtt                                                                                              >  7:136416/1‑136 (MQ=255)
                                                                 tCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCtt                                                                                              >  7:141181/1‑136 (MQ=255)
                                                                 tCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCtt                                                                                              <  8:136416/136‑1 (MQ=255)
                                                                 tCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCtt                                                                                              <  8:141181/136‑1 (MQ=255)
                                                                 tCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGtt                                                                                  <  4:319995/148‑1 (MQ=255)
                                                                             gTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGAttt                                                                       >  6:206971/1‑147 (MQ=255)
                                                                                       cGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTAtgtgt                                                                                                                            <  5:26992/84‑1 (MQ=255)
                                                                                       cGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTAtgtgt                                                                                                                            >  6:26992/1‑84 (MQ=255)
                                                                                         tGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTgg                                                         <  5:206971/149‑1 (MQ=255)
                                                                                            aCGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTgg                                                                                                                                      >  1:205298/1‑69 (MQ=255)
                                                                                            aCGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTgg                                                                                                                                      <  2:205298/69‑1 (MQ=255)
                                                                                            aCGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCt                                                           >  1:50898/1‑144 (MQ=255)
                                                                                            aCGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCt                                                           <  2:50898/144‑1 (MQ=255)
                                                                                                      gcgTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATgc                                             >  8:303999/1‑149 (MQ=255)
                                                                                                        gTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATgccg                                           <  7:303999/149‑1 (MQ=255)
                                                                                                                ccTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGc                                    >  2:188467/1‑148 (MQ=255)
                                                                                                                       ttgagCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGgcgc                            <  6:368073/149‑1 (MQ=255)
                                                                                                                          agCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTca                         >  5:337929/1‑149 (MQ=255)
                                                                                                                                  aaaCCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGgcgc                 <  1:26301/149‑1 (MQ=255)
                                                                                                                                   aaCCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGgcgc                 >  6:171151/1‑148 (MQ=255)
                                                                                                                                     ccGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCAc              <  6:337929/149‑1 (MQ=255)
                                                                                                                                          aTGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGAc         >  3:180725/1‑149 (MQ=255)
                                                                                                                                          aTGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGAc         >  2:96512/1‑149 (MQ=255)
                                                                                                                                          aTGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGAc         >  5:4205/1‑149 (MQ=255)
                                                                                                                                              cgacCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGACCCGa     <  4:180725/149‑1 (MQ=255)
                                                                                                                                               gacCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGACCCGAt    >  4:210281/1‑149 (MQ=255)
                                                                                                                                                acCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCt                           <  2:184264/125‑1 (MQ=255)
                                                                                                                                                 ccTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGACCCGATGa  <  5:171151/149‑1 (MQ=255)
                                                                                                                                                  cTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGATGGAAAAACTAATGCAGCAAAGGCCATGTCGATCAAACGGCGCCACCTGACCCGATGa  >  5:87450/1‑148 (MQ=255)
                                                                                                                                                   tCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGACCCGATGa  >  2:92707/1‑147 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
CGCCGAAGCGCTCCTCCAGTTTGCGTACCTGGTCGGACACCGCAGGCTGGGTAAGAAACAGCCTTTCAGCGGCGCGGGTAAAGCTGCCGTGCACGGCCAC‑CGCGTGGAAAGCCTTGAGTTGAGCGTGGGAAACCGACATGACGACCTCAGTAACAAGCTGGGCTTATGTGTGTAATACGATAAATCGATTTTATTTATCTTACAGCAACTGTTTCCATGGATTTCAGCCCGACGCTGGCACCACGAATGCCGCCAAGGCCATGGCGCTCACACGGCGCCACCTGACCCGATGA  >  NC_002947/2892629‑2892921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑