Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,903,790	C→G	6.1%	G380R (GGG→CGG) ‡	PP_2556 ←	chromate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,903,790	0	C	G	6.1%	208.7 / 3.6	66	G380R (GGG→CGG) ‡	PP_2556	chromate transporter
Reads supporting (aligned to +/- strand):  ref base C (33/29);  new base G (2/2);  total (35/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-01

GTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCTGGTGGGCGCC  >  NC_002947/2903644‑2903937                                                                                                                                                   |                                                                                                                                                     gTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCgg                                                                                                                                                    >  4:40900/1‑149 (MQ=255)        aaCAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTgg                                                                                                                                             >  6:298521/1‑149 (MQ=255)               ccACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTg                                                                                                                                      <  8:145328/149‑1 (MQ=255)                 acaGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGaa                                                                                                                                    <  4:324740/149‑1 (MQ=255)                 acaGCCGCGGCGAGAGCTAACACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGCCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCGCGGTCAGTCGGGCGGTGaa                                                                                                                                    >  2:318156/1‑149 (MQ=255)                  ccgccgccGCGAGCGCTATCCCCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACACCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAAc                                                                                                                                   <  6:218433/147‑1 (MQ=255)                      cgcgGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCa                                                                                                                               <  2:8502/149‑1 (MQ=255)                      cgcgGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCa                                                                                                                               <  2:2686/149‑1 (MQ=255)                             aGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGtt                                                                                                                       <  3:112872/149‑1 (MQ=255)                               cgcTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTtgt                                                                                                                     >  8:254814/1‑149 (MQ=255)                                  tATCACCGCCGAGGACCAGTGGAACGCGCCTTCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCTCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTggg                                                                                                                  >  6:283189/1‑149 (MQ=255)                                     cACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTc                                                                                                                >  7:187398/1‑148 (MQ=255)                                       ccgccgAGGACCAGTGGAACGCGCCATAGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCTCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGAc                                                                                                             <  5:283189/149‑1 (MQ=255)                                         gccgAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTc                                                                                                           <  5:298521/149‑1 (MQ=255)                                              ggACCAGTGGAACGCGCCATCGAACCCGTCCGGCCCCAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTccac                                                                                                        >  1:77567/1‑147 (MQ=255)                                              ggACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCa                                                                                                                                                 <  7:220016/107‑1 (MQ=255)                                              ggACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCa                                                                                                                                                 >  8:220016/1‑107 (MQ=255)                                               gACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCCCACTGACCGGGATCACTGCGGCGGTggtgg                                                                                                                                  >  5:92529/1‑117 (MQ=37)                                               gACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCCCACTGACCGGGATCACTGCGGCGGTggtgg                                                                                                                                  <  6:92529/121‑5 (MQ=37)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTc                                                                                                                >  6:231545/1‑135 (MQ=255)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTc                                                                                                                <  5:231545/135‑1 (MQ=255)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTcca                                                                                                         >  1:220502/1‑142 (MQ=255)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTcca                                                                                                         <  2:220502/142‑1 (MQ=255)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCgg                                                                                                  >  4:150483/1‑149 (MQ=255)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCgg                                                                                                  >  1:300453/1‑149 (MQ=255)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGAATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTgg                                                                                                                   >  2:217641/1‑132 (MQ=255)                                                  cAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGAATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTgg                                                                                                                   <  1:217641/132‑1 (MQ=255)                                                    gTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTc                                                                                                           <  4:100101/138‑1 (MQ=255)                                                    gTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTc                                                                                                           >  3:100101/1‑138 (MQ=255)                                                      ggAACGCGCCATCGAACCCGTCCGGCCACGGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTTCCCCCACCACCGCCGCAGTGATCCCGGTGAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCt                                                                                              <  1:318156/149‑1 (MQ=255)                                                        aaCGCGCCATCGAACCCGTCCGGCCACAGCTCGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTcc                                                                                            >  1:28824/1‑149 (MQ=255)                                                        aaCGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTcc                                                                                            >  8:282677/1‑149 (MQ=255)                                                          cgcgCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCgg                                                                                          <  7:254814/149‑1 (MQ=255)                                                            cgcCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGc                                                                                         >  4:286787/1‑148 (MQ=255)                                                                aTCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGGGCAAGGTTGAGGATTACCCCCTCCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGa                                                                                    <  3:150483/149‑1 (MQ=255)                                                                aTCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGa                                                                                    >  7:151035/1‑149 (MQ=255)                                                                   gAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGa                                                                                 <  2:300453/149‑1 (MQ=255)                                                                      cccGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACa                                                                              <  3:40900/149‑1 (MQ=255)                                                                             ggCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAAc                                                                         >  7:213192/1‑147 (MQ=255)                                                                              gCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAg                                                                      <  6:67087/149‑1 (MQ=255)                                                                               ccACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAgg                                                                     >  3:240000/1‑149 (MQ=255)                                                                                    gCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAg                                                                 >  7:303156/1‑148 (MQ=255)                                                                                              gCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAAcc                                                      <  7:282677/149‑1 (MQ=255)                                                                                              gCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAAc                                                       <  8:187398/148‑1 (MQ=255)                                                                                              gCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAAc                                                       <  2:28824/148‑1 (MQ=255)                                                                                                cGAAGAACAGCGCAAGGTTGAGGATTAACCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGCTCGACTCAACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGCTGAACCAg                                                    >  7:200962/1‑149 (MQ=255)                                                                                                 gaagaaCAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAgg                                                   >  4:12531/1‑149 (MQ=255)                                                                                                    gaaCAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCa                                                >  3:108978/1‑149 (MQ=255)                                                                                                      aCAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCAcc                                              <  4:240000/149‑1 (MQ=255)                                                                                                              aGGTGGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGc                                      <  8:303156/149‑1 (MQ=255)                                                                                                                    aGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGACGGTGAACCAGGTCACCAGGCCCGCTGCCAc                                >  5:297425/1‑149 (MQ=255)                                                                                                                    aGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAg                                                             >  4:45340/1‑120 (MQ=255)                                                                                                                    aGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAg                                                             <  3:45340/120‑1 (MQ=255)                                                                                                                     ggATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACt                               <  8:213192/149‑1 (MQ=255)                                                                                                                      gATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCATGCCCGATGCCGCTg                              >  4:275600/1‑149 (MQ=255)                                                                                                                           cccccACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGTGTCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGcc                          >  1:268662/1‑149 (MQ=255)                                                                                                                           cccccACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGccc                         >  5:139047/1‑149 (MQ=255)                                                                                                                              ccaccaCCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGc                                   >  6:339389/1‑136 (MQ=255)                                                                                                                              ccaccaCCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGc                                   <  5:339389/136‑1 (MQ=255)                                                                                                                              ccaccaCCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCcgc                       >  2:216060/1‑148 (MQ=255)                                                                                                                                  caccGCCCCACTGACCGGGATCACTGCGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGa                                                                                    >  7:181052/1‑83 (MQ=21)                                                                                                                                  caccGCCCCACTGACCGGGATCACTGCGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGa                                                                                    <  8:181052/83‑1 (MQ=21)                                                                                                                                     cgccgcAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGa                                                         <  4:205668/107‑1 (MQ=255)                                                                                                                                     cgccgcAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGa                                                         >  3:205668/1‑107 (MQ=255)                                                                                                                                          cAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCtgg          >  6:52430/1‑149 (MQ=255)                                                                                                                                              gATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCtggtgg       <  8:151035/148‑1 (MQ=255)                                                                                                                                                  ccGGTGAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCTGGTGGGCGcc  <  4:108978/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                     GTAGCGGAACAGCGCCACAGCCGCGGCGAGCGCTATCACCGCCGAGGACCAGTGGAACGCGCCATCGAACCCGTCCGGCCACAGCACGTGGTAGCCGAAGAACAGCGCAAGGTTGAGGATTACCCCCACCACCGCCGCAGTGATCCCGGTCAGTGGGGCGGTGAACTTCAGT‑TCGTTGTGGGTCGACTCCACCAGCGGGCCTCCGGCGAGGATGAACAGGAACGAGGGCAGGAAGGTGAACCAGGTCACCAGGCCCGCTGCCACTGCGCCCGCGAGGAACGCCTGGTGGGCGCC  >  NC_002947/2903644‑2903937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑