Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,921,460	G→A	100%	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	100.0%	90.7 / NA	28	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921314‑2921605                                                                                                                                                   |                                                                                                                                                   acccgccgCTGGGGGAGGCCGGTAACGACAACCTCAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagg                                                                                                                                                  <  2:344426/142‑1 (MQ=255) acacGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagg                                                                                                                                                  >  4:251230/1‑149 (MQ=255)                      gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGcc                                                                                                                                            >  5:297198/1‑134 (MQ=255)                      gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGcc                                                                                                                                            <  6:297198/134‑1 (MQ=255)                               aCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCATAGGTGCg                                                                                                                    >  3:131616/1‑149 (MQ=255)                                ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                   >  3:107491/1‑149 (MQ=255)                                           gcgcTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGAt                                                                                                        <  5:151726/149‑1 (MQ=255)                                               tGGCGATTACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGTCGGCGATAGTAACGAAACATTATTCGGCGACGTGGGTACCGACACCCTGATAAGGGGGGCCGGAAACTACTTCCTCAACGGAGCGGCAGGTAACGATACgg                                                                                                    >  5:257695/1‑149 (MQ=255)                                                  cGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTc                                                                                                                             <  2:318742/121‑1 (MQ=255)                                                  cGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTc                                                                                                                             >  1:318742/1‑121 (MQ=255)                                                   gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtggt                                                                                                <  3:251230/149‑1 (MQ=255)                                                       accgCCTGGATGGCGGCAACGGCAATGACAACCTGCACGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGTCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggt                                                                                            <  4:131616/146‑1 (MQ=255)                                                                      gcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTCATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa                                                                              >  4:190987/1‑148 (MQ=255)                                                                                          gctgggggcgTTGGGAACGCCACCTTTTTGCGCGCAGTTGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGAt                                                           <  5:1247/141‑1 (MQ=255)                                                                                                            gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                                    <  3:154018/120‑1 (MQ=255)                                                                                                            gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                                    >  4:154018/1‑120 (MQ=255)                                                                                                                     ttCGGCGAAGTGGGTACCGACAACCTGATCAGGGGGGCAGGCAACGACTTCCTCAACGTTGCGGAAGTTAACGATACGGTGGTGGGGGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAgg                             <  6:257695/149‑1 (MQ=255)                                                                                                                       cGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCt                                  <  7:165556/142‑1 (MQ=255)                                                                                                                          cGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg                        <  4:107491/149‑1 (MQ=255)                                                                                                                                gggTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc                   >  2:210751/1‑148 (MQ=255)                                                                                                                                  gTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAc                >  8:90788/1‑149 (MQ=255)                                                                                                                                   tACCGACACCCTGATCAGGGGGGCCGACAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAcc               >  5:159704/1‑149 (MQ=255)                                                                                                                                      cGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGa            >  6:289813/1‑149 (MQ=255)                                                                                                                                        acacCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGCGAAACGATGTGTTCCAGTTCGCTGCAg                              <  1:15137/130‑1 (MQ=255)                                                                                                                                        acacCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAg                              >  2:15137/1‑129 (MQ=255)                                                                                                                                         cacCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatca         <  3:190987/149‑1 (MQ=255)                                                                                                                                            catGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTCACGATACGTTGGTGGGTGGGGCCGGTAGCGAGACCCTGATCGCGACGGAGG‑GAACCGATGTCTTCCGGGTCTCTGCAGGCTATGGCCACGACCTGgtcagct      >  8:254664/3‑146 (MQ=255)                                                                                                                                             cTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGAtcatca      >  1:139841/1‑148 (MQ=255)                                                                                                                                              tGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAt    >  3:74614/1‑149 (MQ=255)                                                                                                                                                aTCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAttt  >  6:323693/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATG‑GAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATTT  >  NC_002947/2921314‑2921605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑