Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,998,793 G→A 8.0% G65G (GGC→GGT)  PP_2622 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,998,7930GA 8.0% 140.8 / 2.4 50G65G (GGC→GGT) PP_2622hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (21/25);  new base A (2/2);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.56e-01

CCAGGCAATCCAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCGGGAGGCCTC  >  NC_002947/2998657‑2998939
                                                                                                                                         |                                                                                                                                                  
ccAGGCAATCCAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATGGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACg                                                                                                                                        >  1:253162/1‑149 (MQ=255)
ccAGGCAATCCAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCAc                                                                                                                                         <  6:259711/148‑1 (MQ=255)
 cAGGCAATCCAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGt                                                                                                                                       >  4:168876/1‑149 (MQ=255)
  aGGCAATCCAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGt                                                                                                                                       >  2:264631/1‑148 (MQ=255)
        tCCAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGcc                                                                                                                                 >  2:345691/1‑148 (MQ=255)
          cAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCAt                                                                                                                              <  7:232991/149‑1 (MQ=255)
                  aaTGGAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGCATCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTc                                                                                                                       <  1:264631/149‑1 (MQ=255)
                     gCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTcgcg                                                                                                                    >  4:5233/1‑148 (MQ=255)
                       aGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAg                                                                                                                 <  6:192116/149‑1 (MQ=255)
                            cAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTcgc                                                                                                                     <  4:215550/140‑1 (MQ=255)
                            cAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTcgc                                                                                                                     >  3:215550/1‑140 (MQ=255)
                            cAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGAGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCACCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGcc                                                                                                              >  6:254942/1‑147 (MQ=255)
                                  ggCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAg                                                                                                      >  6:290176/1‑149 (MQ=255)
                                  ggCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAg                                                                                                      <  2:297757/149‑1 (MQ=255)
                                          tATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGctc                                                                                              >  8:278120/1‑149 (MQ=255)
                                                   cTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCAc                                                                                     >  2:194550/1‑149 (MQ=255)
                                                        ttGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGAGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGATCTGGGGGCACCCCTg                                                                                >  3:317985/1‑149 (MQ=255)
                                                            gcgtgcgtACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCgg                                                                            <  2:253162/149‑1 (MQ=255)
                                                            gcgtgcgtACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCACCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCgg                                                                            >  6:27087/1‑149 (MQ=255)
                                                              gtgcgtACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCggg                                                                           >  3:295809/1‑148 (MQ=255)
                                                              gtgcgtACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGc                                                                          <  7:152394/149‑1 (MQ=255)
                                                                 cgtACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCACCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCac                                                                        <  5:254942/148‑2 (MQ=255)
                                                                  gtACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTc                                                                      <  3:5233/149‑1 (MQ=255)
                                                                   tACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTca                                                                     <  3:168876/149‑1 (MQ=255)
                                                                    aCCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTcag                                                                    >  5:18459/1‑149 (MQ=255)
                                                                     ccTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTcag                                                                    <  5:307573/148‑1 (MQ=255)
                                                                      cTGTATGCGCGGCGCGAAGCGCTGGAGGGTGCGGCGGATAGCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTcagca                                                                  <  7:274859/149‑1 (MQ=255)
                                                                         tATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGTGGGCATTCagcagc                                                                <  6:294371/148‑1 (MQ=255)
                                                                            gcgcGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGttt                                                            >  2:358842/1‑149 (MQ=255)
                                                                                ggCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAg                                                        <  4:295809/149‑1 (MQ=255)
                                                                                 gCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCACCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAgg                                                       <  5:27087/149‑1 (MQ=255)
                                                                                     gAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGGGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTgg                                                    <  4:317985/148‑1 (MQ=255)
                                                                                          gcTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTg                                              <  1:345691/149‑1 (MQ=255)
                                                                                              gAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATgg                                          <  1:358842/149‑1 (MQ=255)
                                                                                                       gcggATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACt                                 >  3:296818/1‑149 (MQ=255)
                                                                                                           atatCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTtgtg                             <  7:278120/149‑1 (MQ=255)
                                                                                                                cgcgC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCATGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGaa                         <  3:317007/148‑1 (MQ=255)
                                                                                                                   gc‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCGCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAgcgc                     <  5:36692/149‑1 (MQ=255)
                                                                                                                           tGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCg              >  5:269310/1‑149 (MQ=255)
                                                                                                                              acaAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCg           >  1:233668/1‑149 (MQ=255)
                                                                                                                                aagcaagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCg                                                                             <  3:268138/81‑1 (MQ=255)
                                                                                                                                aagcaagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCg                                                                             >  4:268138/1‑81 (MQ=255)
                                                                                                                                aagcaagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCgg          >  2:305568/1‑148 (MQ=255)
                                                                                                                                aagcaagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCgg          >  8:134391/1‑148 (MQ=255)
                                                                                                                                 agcaagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCGGGa        <  6:18459/149‑1 (MQ=255)
                                                                                                                                  gcaagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTgg                                                                               >  7:212584/1‑77 (MQ=255)
                                                                                                                                  gcaagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTgg                                                                               <  8:212584/77‑1 (MQ=255)
                                                                                                                                   caagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCGGGAgg      <  2:233668/149‑1 (MQ=255)
                                                                                                                                   caagTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCGGGAgg      >  8:167582/1‑149 (MQ=255)
                                                                                                                                       tCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCGGGAGGCctc  <  7:167582/149‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                  
CCAGGCAATCCAGCCTGAAATGCAGCTCCAGCGGGGCATGGGTATCGGGCACTGCGTTGAGCGTGCGTACCTGTATGCGCGGCTCGAAGCGCTGGAGGGTGCGGCGGATATCCGCGC‑TCACCTGCACAAGCAAGTCGCCGTTGCTCACGTCATGCCCATTGAAGTCGCGCAGGCCCAGCTCAGGGCTGCTCTGGGAGCACCCCTGGCGGGCATTCAGCAGCGTTTCGAGGTGGCGCTTGATGGCGGCGAACTTGTGGGAAGCGCTCTCCCGTCGGGAGGCCTC  >  NC_002947/2998657‑2998939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑