Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,011,520	G→A	8.7%	R194Q (CGG→CAG)	bcsG →	endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,011,520	0	G	A	8.7%	129.7 / 2.2	46	R194Q (CGG→CAG)	bcsG	endoglucanase
Reads supporting (aligned to +/- strand):  ref base G (20/22);  new base A (2/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.46e-01

GTCGGCGGCTTGCCGTCGCTGGTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCACTGTTGTCGCGCATGGACGTGATCTTCGA  >  NC_002947/3011375‑3011664                                                                                                                                                  |                                                                                                                                                 gtcggCGGCTTGCCGTCGCTGGTGGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGt                                                                                                                                               <  4:206568/149‑1 (MQ=255)       ggcTTGCCGGCGCTGGTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGc                                                                                                                                         <  2:312489/149‑1 (MQ=255)            gCCGTCGCTGGTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTc                                                                                                                                     <  2:41668/148‑1 (MQ=255)                 cGCTGGTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGcc                                                                                                                               >  8:318011/1‑149 (MQ=255)                  gCTGGTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCgg                                                                                                                                                 <  7:260361/130‑1 (MQ=255)                  gCTGGTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCgg                                                                                                                                                 >  8:260361/1‑130 (MQ=255)                      gTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGa                                                                                                                           <  6:262884/148‑1 (MQ=255)                      gTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGa                                                                                                                           <  8:114867/148‑1 (MQ=255)                       ttGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGt                                                                                                                         <  7:318011/149‑1 (MQ=255)                          caggccaggcGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGc                                                                                                                       <  4:208449/148‑1 (MQ=255)                             gccaggcGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGAt                                                                                                                   <  1:265716/149‑1 (MQ=255)                                 ggcGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAg                                                                                                                >  7:45127/1‑148 (MQ=255)                                  gcgcCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGc                                                                                                               >  7:144019/1‑148 (MQ=255)                                                 gcgcGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACctgct                                                                                               >  3:198832/1‑149 (MQ=255)                                                         cGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGc                                                                                                               >  6:246784/1‑125 (MQ=255)                                                         cGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGc                                                                                                               <  5:246784/125‑1 (MQ=255)                                                         cGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCAGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATc                                                                                        >  8:293706/1‑148 (MQ=255)                                                           cAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGc                                                                                                               <  4:30015/123‑1 (MQ=255)                                                           cAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGc                                                                                                               >  3:30015/1‑123 (MQ=255)                                                           cAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCaa                                                                                      >  6:285568/1‑148 (MQ=255)                                                              ccGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATc                                                                                  >  7:284759/1‑149 (MQ=255)                                                                    ccGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCg                                                                            >  7:79384/1‑149 (MQ=255)                                                                            gCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGt                                                                                            >  1:289679/1‑125 (MQ=255)                                                                            gCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGt                                                                                            <  2:289679/125‑1 (MQ=255)                                                                             ccACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTgg                                                                   >  8:255999/1‑149 (MQ=255)                                                                             ccACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTg                                                                    <  4:198832/148‑1 (MQ=255)                                                                              cACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCAGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTggg                                                                  <  7:293706/149‑1 (MQ=255)                                                                                cTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGAt                                                                <  5:285568/149‑1 (MQ=255)                                                                                   cagcagCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGAc                                                             <  6:173147/149‑1 (MQ=255)                                                                                   cagcagCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGAc                                                             <  8:284759/149‑1 (MQ=255)                                                                                        gcCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTc                                                        >  8:122579/1‑149 (MQ=255)                                                                                         ccGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTcc                                                       <  1:352096/149‑1 (MQ=255)                                                                                                tGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTgg                                                <  8:79384/149‑1 (MQ=255)                                                                                                 gCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTggg                                               >  1:319842/1‑149 (MQ=255)                                                                                                     cGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTg                                           >  2:216529/1‑149 (MQ=255)                                                                                                      gCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGc                                          <  1:216529/149‑1 (MQ=255)                                                                                                          gACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTACGGGa                                      <  5:146218/149‑1 (MQ=255)                                                                                                             aGCTGGCTTGAGCGCTTCTTCGGCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACaa                                   >  7:52647/1‑149 (MQ=255)                                                                                                                  gCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCAc                              <  5:261469/149‑1 (MQ=255)                                                                                                                  gCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCAc                              >  3:49869/1‑149 (MQ=255)                                                                                                                    ttGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCACtg                            >  4:70414/1‑149 (MQ=255)                                                                                                                           cttcttCGCCAGTGAGCGCGAGGGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGAc                                                             >  7:225634/1‑109 (MQ=255)                                                                                                                            ttcttcGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACa                                    <  4:41494/133‑1 (MQ=255)                                                                                                                                 cGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCACTGTTGTCGCGCa                  <  8:52647/146‑1 (MQ=255)                                                                                                                                       tGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCACTGTTGTCGCGCATGGACGTGa         >  3:195903/1‑149 (MQ=255)                                                                                                                                       tGAGCGCGAGCAGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGAc                                                             >  7:33350/1‑97 (MQ=255)                                                                                                                                       tGAGCGCGAGCAGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGAc                                                             <  8:33350/97‑1 (MQ=255)                                                                                                                                              gAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCACTGTTGTCGCGCATGGACGTGATCTTCGa  >  3:51719/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 GTCGGCGGCTTGCCGTCGCTGGTTGCAGGCCAGGCGCCCGGCGTGGCGGCGCGTACCGCAGCCGAACCCGCGCCTGCCACTGCAGCAGCCGACAATGCCACGCTCGACAGCTGGCTTGAGCGCTTCTTCGCCAGTGAGCGCGAGCGGGTGACGGCGTTCCCTGCCGTGAGTGCCGATGAGCAACCGTTCGACCTGCTGGTAATCAACATCTGCTCGCTGGCCTGGGATGACCTGTCCGCCGTGGGGCTGCGGGACAACCCACTGTTGTCGCGCATGGACGTGATCTTCGA  >  NC_002947/3011375‑3011664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑