Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,346	T→G	6.9%	T13P (ACG→CCG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,346	0	T	G	6.9%	219.3 / 6.4	73	T13P (ACG→CCG)	trmE	GTPase
Reads supporting (aligned to +/- strand):  ref base T (39/28);  new base G (2/3);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

ACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAG  >  NC_002947/6207‑6493                                                                                                                                             |                                                                                                                                                    aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                                            <  6:148016/149‑3 (MQ=255)  ccAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtgg                                                                                                                                           <  8:182581/149‑4 (MQ=255)   cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                                          <  7:329004/149‑1 (MQ=255)   cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                                          >  4:301441/1‑149 (MQ=255)   cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGGCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGCGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcgg                                                                                                                                          >  8:58336/1‑149 (MQ=255)        cTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGAt                                                                                                                                      <  1:89040/149‑1 (MQ=255)            aTCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGtt                                                                                                                                  >  6:67205/1‑148 (MQ=255)            aTCGTCGCGGAATGGACCGTAATGGGAATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCTGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGtt                                                                                                                                  >  8:155248/1‑148 (MQ=255)             tcgtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTc                                                                                                                                <  7:3786/149‑1 (MQ=255)                           aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTc                                                                                                                   <  8:143173/148‑1 (MQ=255)                           aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCa                                                                                                                  >  4:352612/1‑149 (MQ=255)                               tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGAGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATgat                                                                                                              >  6:360836/1‑147 (MQ=255)                                   gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                                            >  5:334034/1‑113 (MQ=38)                                   gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgtg                                                                                                                                            <  6:334034/115‑3 (MQ=38)                                    ggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                         <  3:352612/149‑1 (MQ=255)                                       aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                                      >  7:300213/1‑149 (MQ=255)                                       aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                                      >  8:86748/1‑149 (MQ=255)                                          ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                             <  6:327852/139‑1 (MQ=255)                                          ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                             >  5:327852/1‑139 (MQ=255)                                           cgcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGGCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGGCGTGGCGATTGCGGCGATGGTTTCACGGACAGTGGTCATGCTCGAAAGCCTCTa                                                                                                  <  5:360836/149‑1 (MQ=255)                                            gcgGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                                 >  4:334401/1‑149 (MQ=255)                                                  tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaa                                                                                            >  5:345328/1‑148 (MQ=255)                                                      ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                                        >  8:34920/1‑148 (MQ=255)                                                        tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                                     <  7:86748/149‑1 (MQ=255)                                                            gCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                                 >  7:311462/1‑149 (MQ=255)                                                            gCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                                 <  7:155248/149‑1 (MQ=255)                                                              cGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGa                                                                                  >  3:131079/1‑146 (MQ=255)                                                                 tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCa                                                                             >  5:205462/1‑148 (MQ=255)                                                                      aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                                        <  3:334401/148‑1 (MQ=255)                                                                       aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                                      >  2:334053/1‑149 (MQ=255)                                                                           gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                                  >  2:131220/1‑149 (MQ=255)                                                                           gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                                  >  6:224936/1‑149 (MQ=255)                                                                           gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                                  >  5:81461/1‑149 (MQ=255)                                                                           gccggccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCAAGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCa                                                                  >  8:277016/1‑149 (MQ=255)                                                                                ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                         >  7:64888/1‑105 (MQ=255)                                                                                ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                         <  8:64888/105‑1 (MQ=255)                                                                                ccTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAgg                                                             <  5:67205/149‑1 (MQ=255)                                                                                    ggCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAgggg                                                           <  6:345328/147‑1 (MQ=255)                                                                                      cAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                               <  1:62537/109‑1 (MQ=255)                                                                                      cAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGa                                                                                               >  2:62537/1‑109 (MQ=255)                                                                                        gCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGttt                                                     <  4:310833/149‑1 (MQ=255)                                                                                               ggCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTc                                              <  6:205462/149‑1 (MQ=255)                                                                                                          ccTGACAATTCCCACACCGCCCCGGCCCTGGGCAGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTAATGCTCGAAAGCCTCTACGACAAAAACGACAGAAAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTAACAGGATAGc                                    >  2:71735/1‑149 (MQ=255)                                                                                                           cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                                    <  4:227367/68‑1 (MQ=255)                                                                                                           cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                                    >  3:227367/1‑68 (MQ=255)                                                                                                           cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCg                                   >  1:45881/1‑149 (MQ=255)                                                                                                             gACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGAGGTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACAAGGGGGCGTTTATTATTCACAGGCTAGCGTa                                 >  4:237769/1‑149 (MQ=255)                                                                                                              aCAATTCCCACACCGCCCCGGCCCTGGGCGGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTa                                 <  2:283139/148‑1 (MQ=255)                                                                                                               cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAtt                               <  5:224936/149‑1 (MQ=255)                                                                                                               cAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAtt                               <  2:76934/149‑1 (MQ=255)                                                                                                                   tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTcaca                                           >  2:292739/1‑133 (MQ=255)                                                                                                                   tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGCCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTcaca                                           <  1:292739/133‑1 (MQ=255)                                                                                                                     ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg                         >  6:107083/1‑149 (MQ=255)                                                                                                                     ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCg                         >  6:258032/1‑149 (MQ=255)                                                                                                                      cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                                    <  1:57929/121‑1 (MQ=255)                                                                                                                      cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                                    >  2:57929/1‑121 (MQ=255)                                                                                                                      cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCCCAGGCTAGCGTATTAGCCCGt                        >  8:67483/1‑149 (MQ=255)                                                                                                                       ccatcGCCACGGCCCAGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt                        >  7:90100/5‑148 (MQ=255)                                                                                                                       acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAACAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc                       <  3:301441/149‑1 (MQ=255)                                                                                                                       acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCCCTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt                        >  3:222117/1‑148 (MQ=255)                                                                                                                         acCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCaa                     <  2:150373/149‑1 (MQ=255)                                                                                                                           cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag                    >  4:59370/1‑148 (MQ=255)                                                                                                                             ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag                 >  2:52805/1‑149 (MQ=255)                                                                                                                             ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag                 >  5:234092/1‑149 (MQ=255)                                                                                                                              cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag                 >  2:186182/1‑148 (MQ=255)                                                                                                                              cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag                 <  1:186182/148‑1 (MQ=255)                                                                                                                                cGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagcag              >  8:208680/1‑149 (MQ=255)                                                                                                                                cGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagcag              >  4:32975/1‑149 (MQ=255)                                                                                                                                    ccTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCttt          <  1:52805/149‑1 (MQ=255)                                                                                                                                       gggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCtttttt       >  7:125863/1‑149 (MQ=255)                                                                                                                                        ggCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTg      <  3:32975/149‑1 (MQ=255)                                                                                                                                         gCCGTGGCGATGGCGGCGATTGTTTCACTCACAGTTTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATATCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTgg     <  1:71735/149‑1 (MQ=255)                                                                                                                                            gTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAg  >  2:69252/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                    ACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGAC‑AACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTTGGTAG  >  NC_002947/6207‑6493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑