Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,068,346 A→G 7.6% K462R (AAG→AGG)  qedH‑II → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,068,3460AG 7.6% 146.2 / 2.5 54K462R (AAG→AGG) qedH‑IIquinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (25/24);  new base G (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGCCAAGAACTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGC  >  NC_002947/3068199‑3068494
                                                                                                                                                   |                                                                                                                                                    
tCGGCGCCAAGAACTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAg                                                                                                                                                     >  3:141084/1‑149 (MQ=255)
     gcCAAGAACTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCt                                                                                                                                                >  1:4343/1‑149 (MQ=255)
         aGAACTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAAt                                                                                                                                            <  8:339724/149‑1 (MQ=255)
                gATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGa                                                                                                                                       <  8:238499/147‑1 (MQ=255)
                  tGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTa                                                                                                                                    >  5:129099/1‑148 (MQ=255)
                             tACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGtgct                                                                                                                        >  4:5975/1‑149 (MQ=255)
                             tACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTGCACCCTCACGCCGCTCAATGAGGCCGACATCGGCGggct                                                                                                                        >  4:11003/1‑149 (MQ=255)
                                 acaaGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTgcgc                                                                                                                    <  4:12827/149‑1 (MQ=255)
                                        cacaGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCg                                                                                                             <  2:228238/149‑1 (MQ=255)
                                           aGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGAcc                                                                                                          <  2:4343/149‑1 (MQ=255)
                                                     tACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGtgctgc                                                                                                                      >  4:52606/1‑127 (MQ=255)
                                                     tACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGtgctgc                                                                                                                      <  3:52606/127‑1 (MQ=255)
                                                        gTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCaa                                                                                             <  4:141084/149‑1 (MQ=255)
                                                             gTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAg                                                                                        <  3:264611/149‑1 (MQ=255)
                                                              tCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCCTCGACCCGGTCAGCGGCAAGGAAgt                                                                                       >  8:299170/1‑149 (MQ=255)
                                                              tCCAACAAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAgt                                                                                       >  1:310096/1‑149 (MQ=255)
                                                                 aaCGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGtgtg                                                                                    >  6:308209/1‑149 (MQ=255)
                                                                   cGAGTGGGGCATGGACATCTTGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGgc                                                                                  <  3:5975/149‑1 (MQ=255)
                                                                   cGAGTGGGGCATGGACATCTGGAAGGAAGGCATCGCCTATAAGAAAGGGGCGGCGGTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGGCAGCGGCAAGGAAGTGTGgc                                                                                  <  3:11003/149‑1 (MQ=255)
                                                                           gCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAGGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCcac                                                                             >  5:82198/1‑146 (MQ=255)
                                                                           gCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAgt                                                                                       >  1:134590/1‑136 (MQ=255)
                                                                           gCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAgt                                                                                       <  2:134590/136‑1 (MQ=255)
                                                                           gCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAg                                                                          <  6:129099/149‑1 (MQ=255)
                                                                                 aCATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAGGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAgtgt                                                                                     <  3:345857/132‑1 (MQ=255)
                                                                                 aCATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAGGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAgtgt                                                                                     >  4:345857/1‑132 (MQ=255)
                                                                                   aTCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTAt                                                                    >  2:307402/1‑147 (MQ=255)
                                                                                   aTCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATgc                                                                  >  3:309815/1‑149 (MQ=255)
                                                                                   aTCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATgc                                                                  >  8:120873/1‑149 (MQ=255)
                                                                                        gAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGcgccg                                                              <  2:310096/148‑1 (MQ=255)
                                                                                           cGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAGGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCtgt                                                          <  6:82198/149‑1 (MQ=255)
                                                                                            gAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCtgtg                                                         <  2:98808/149‑1 (MQ=255)
                                                                                                   tCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGgt                                                  >  1:152933/1‑149 (MQ=255)
                                                                                                      ccTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGtgctgc                                                                                                                      <  3:202762/78‑1 (MQ=255)
                                                                                                      ccTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGtgctgc                                                                                                                      >  4:202762/1‑78 (MQ=255)
                                                                                                      ccTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGtgtg                                               >  1:72701/1‑149 (MQ=255)
                                                                                                           aaGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGac                                          >  7:92333/1‑149 (MQ=255)
                                                                                                                       gcgTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCaa                              >  4:31231/1‑149 (MQ=255)
                                                                                                                        cgTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAAc                             <  2:152933/149‑1 (MQ=255)
                                                                                                                            ccTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTgg                         >  2:77678/1‑149 (MQ=255)
                                                                                                                             cTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGacc                                         >  1:169511/1‑132 (MQ=255)
                                                                                                                             cTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGacc                                         <  2:169511/132‑1 (MQ=255)
                                                                                                                             cTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGgt                        >  4:270087/1‑149 (MQ=255)
                                                                                                                              tCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCTCCAAGGGCAACCTGgtg                       >  8:193941/1‑149 (MQ=255)
                                                                                                                               cGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGc                                              <  5:322377/125‑1 (MQ=255)
                                                                                                                               cGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGc                                              >  6:322377/1‑125 (MQ=255)
                                                                                                                                 gTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCTCCAAGGGCAACCTGGTGTTc                    <  7:193941/149‑1 (MQ=255)
                                                                                                                                     cGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACgg                <  8:92333/149‑1 (MQ=255)
                                                                                                                                            aCCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGc          <  1:307402/148‑1 (MQ=255)
                                                                                                                                              cATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGgt                        >  6:50039/1‑132 (MQ=255)
                                                                                                                                              cATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGgt                        <  5:50039/132‑1 (MQ=255)
                                                                                                                                               aTCAAGCCGCTCAATGAAGACTACATGGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCa        >  1:191184/1‑147 (MQ=255)
                                                                                                                                                tCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTg                          <  2:10043/128‑1 (MQ=255)
                                                                                                                                                tCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTg                          >  1:10043/1‑128 (MQ=255)
                                                                                                                                                 cAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAgg    <  7:120873/149‑1 (MQ=255)
                                                                                                                                                   aGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGc  >  1:3602/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCGGCGCCAAGAACTGGATGCCGATGGCCTACAACAAGGACACAGGGCTGTTCTACGTGCCGTCCAACGAGTGGGGCATGGACATCTGGAACGAAGGCATCGCCTATAAGAAAGGTGCGGCGTTCCTCGGTGCCGGCTTCACCATCAAGCCGCTCAATGAAGACTACATCGGCGTGCTGCGCGCCATCGACCCGGTCAGCGGCAAGGAAGTGTGGCGCCACAAGAACTATGCGCCGCTGTGGGGCGGTGTGCTGACCACCAAGGGCAACCTGGTGTTCACGGGCACGCCAGAGGGC  >  NC_002947/3068199‑3068494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑