Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,087,768 G→C 7.4% R259R (CGC→CGG) ‡ metR‑II ← homocysteine‑binding DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,087,7680GC 7.4% 168.6 / 3.6 54R259R (CGC→CGG) ‡metR‑IIhomocysteine‑binding DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (24/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

AACCGGGCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTCGGCGGG  >  NC_002947/3087621‑3087914
                                                                                                                                                   |                                                                                                                                                  
aaCCGGGCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGgc                                                                                                                                                   >  7:283173/1‑149 (MQ=255)
   cGGGCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCt                                                                                                                                                <  4:43618/149‑1 (MQ=255)
             gCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCtt                                                                                                                                               <  6:29623/140‑1 (MQ=255)
             gCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCtt                                                                                                                                               >  5:29623/1‑140 (MQ=255)
               cACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACa                                                                                                                                     >  8:33857/1‑148 (MQ=255)
               cACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACa                                                                                                                                     >  7:254922/1‑148 (MQ=255)
                aCGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATa                                                                                                                                   <  5:157381/149‑1 (MQ=255)
                    tAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCcg                                                                                                                               >  3:136522/1‑149 (MQ=255)
                        acCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCcgcgc                                                                                                                            >  2:351634/1‑148 (MQ=255)
                        acCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCCTTGGCgagc                                                                                                                                        >  1:22620/1‑133 (MQ=255)
                        acCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGCGCCTTGGCgagc                                                                                                                                        <  2:22620/136‑4 (MQ=255)
                              cAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCACCAGCATGTCACTGCGCAGTGCAGCAAAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAAcgg                                                                                                                      >  2:320620/1‑146 (MQ=255)
                                 cGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTAtt                                                                                                                  <  5:358193/149‑1 (MQ=255)
                                     gtgAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGt                                                                                                               <  6:300052/148‑1 (MQ=255)
                                          cGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACgg                                                                                                         >  3:148795/1‑149 (MQ=255)
                                                gTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGt                                                                                                   >  2:270981/1‑149 (MQ=255)
                                                   cTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTgg                                                                                                <  8:254922/149‑1 (MQ=255)
                                                              gCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGgcagca                                                                                      <  4:136522/148‑1 (MQ=255)
                                                              gCAGAAAATCACGCATGTACGGCACCTCCAGCATGCCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGgcaggcag                                                                                        >  2:106626/1‑146 (MQ=255)
                                                               cAGAAAATCACGCATGTGCGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGa                                                                                    >  5:281279/1‑149 (MQ=255)
                                                               cAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGa                                                                                    >  6:199943/1‑149 (MQ=255)
                                                                   aaaTCACGCATGTACGGCACCTCCAGCATGCCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGAcgcc                                                                                <  1:106626/149‑1 (MQ=255)
                                                                    aaTCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCCGCAGAcgccg                                                                               >  7:330932/1‑149 (MQ=255)
                                                                            aTGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACt                                                                                                                       >  8:101794/1‑101 (MQ=255)
                                                                            aTGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCgg                                                                        >  6:364300/1‑148 (MQ=255)
                                                                            aTGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTGGGCGGTTACATAGCCGCGCGAACt                                                                                                                       <  7:101794/101‑1 (MQ=255)
                                                                             tGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGaa                                                                      <  5:199943/149‑1 (MQ=255)
                                                                             tGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGaa                                                                      <  1:351634/149‑1 (MQ=255)
                                                                             tGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGaa                                                                      <  5:364300/149‑1 (MQ=255)
                                                                              gTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGaa                                                                      <  8:13055/148‑1 (MQ=255)
                                                                                      ccTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGt                                                             <  8:283173/149‑1 (MQ=255)
                                                                                                 tCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGcatcatcat                                                  <  4:148795/149‑1 (MQ=255)
                                                                                                        gcAGTGCAGCATAGAGGGTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTc                                           <  3:299087/149‑1 (MQ=255)
                                                                                                           gTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGt                                        >  7:33861/1‑149 (MQ=255)
                                                                                                            tGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCTGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGtt                                       <  6:281279/149‑1 (MQ=255)
                                                                                                                   tAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAgt                                <  1:270981/149‑1 (MQ=255)
                                                                                                                    ngagGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAgt                                >  4:315161/2‑148 (MQ=255)
                                                                                                                        gTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAATCAACCa                                                               >  3:51/1‑113 (MQ=255)
                                                                                                                        gTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCa                                                               <  4:51/113‑1 (MQ=255)
                                                                                                                         tttGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAgtacgtac                          <  7:33857/149‑1 (MQ=255)
                                                                                                                            gCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCACGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCt                        <  6:180165/148‑1 (MQ=255)
                                                                                                                               tGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTg                                    >  6:33961/1‑133 (MQ=255)
                                                                                                                               tGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTg                                    <  5:33961/133‑1 (MQ=255)
                                                                                                                               tGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGc                    >  8:284760/1‑149 (MQ=255)
                                                                                                                                gCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCg                   >  4:7713/1‑149 (MQ=255)
                                                                                                                                 cAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGggcagg                                                                                           >  6:41496/1‑76 (MQ=255)
                                                                                                                                    gCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGcatc                                                       <  7:276841/109‑1 (MQ=255)
                                                                                                                                    gCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGcatc                                                       >  8:276841/1‑109 (MQ=255)
                                                                                                                                     ccGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTc              <  3:7713/149‑1 (MQ=255)
                                                                                                                                           ccGCCAAGCGCCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGAc           <  7:50103/145‑1 (MQ=255)
                                                                                                                                           ccGCCAAGCGCCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGAc           >  8:50103/2‑146 (MQ=255)
                                                                                                                                             gCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTcgg      >  2:166346/1‑149 (MQ=255)
                                                                                                                                               cAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCt                                     <  3:220763/116‑1 (MQ=255)
                                                                                                                                               cAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCt                                     >  4:220763/1‑116 (MQ=255)
                                                                                                                                                 aGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTCGGCggg  <  3:315161/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACCGGGCCTGACGCCACGCTAACACCTGCCAGCGTGGTGAACGAGATGTCCTTGGCCGTGAGCAGAAAATCACGCATGTACGGCACCTCCAGCATGTCACTGCGCAGTGCAGCATAGAGGTTTGCCTGCAGGCCGTGCTCGCCAAGGCGCTTGGCGGTTACATAGCCGCGCGAACTGTATTCGTGCACGGCCCAGTTGGGCAGGCAGCAGACGCCGCGCCCGGAAGCAACCAGTTGCATCATCATCACGGTCAGTTCTGAAGTACGTACCTGTGCGGGCTCGACGTCGGCGGG  >  NC_002947/3087621‑3087914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑