Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,116,175 G→A 9.5% G305S (GGC→AGC)  PP_2732 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,116,1750GA 9.5% 113.6 / 2.6 42G305S (GGC→AGC) PP_2732hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (17/21);  new base A (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

GTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCGGGC  >  NC_002947/3116033‑3116319
                                                                                                                                              |                                                                                                                                                
gTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGc                                                                                                                                            >  5:108301/1‑149 (MQ=255)
  aCCTGTTTCATGCGCGCATCAGGGCGGCGCTCAACCTCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAACCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                          <  4:189873/149‑1 (MQ=255)
    cTGTTTCATGCGCGCATCAGTGCGGCGCACAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                          >  1:202767/1‑147 (MQ=255)
     ggTTTCATGCGCGCATCTGTGCGGCGCTCAACATCGGCCTTCTGGTTGTGCGGCGTCTGTGCGAGGACGTCGACAATGCATGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGCgaatg                                                                                                                                       <  6:328101/148‑5 (MQ=255)
       tttCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGCgaatgag                                                                                                                                     >  7:140032/1‑143 (MQ=255)
          cATGCGCGCATCAGTGCGGCGCTCAACATCNGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTAcgt                                                                                                                                  >  1:33858/1‑149 (MQ=255)
                 gcATCAGTGCGGCGCTCAACATCGGCCTTCTGGAGGTGCGGCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGc                                                                                                                           <  1:226691/149‑1 (MQ=255)
                      aGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCTACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCt                                                                                                                       <  5:179449/148‑1 (MQ=255)
                        tGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTAtt                                                                                                                    <  2:69825/149‑1 (MQ=255)
                         gcggcgCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTg                                                                                                                   >  6:66511/1‑149 (MQ=255)
                                  aaCATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGc                                                                                                                                            <  4:103267/115‑1 (MQ=255)
                                  aaCATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCCGATCAGCTGGc                                                                                                                                            >  3:103267/1‑115 (MQ=255)
                                    cATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGc                                                                                                        >  3:327656/1‑149 (MQ=255)
                                     aTCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGcc                                                                                                       >  1:243466/1‑149 (MQ=255)
                                       cGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCgg                                                                                                     >  8:303981/1‑149 (MQ=255)
                                        ggCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGCGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCgg                                                                                                     <  8:158230/148‑1 (MQ=255)
                                              cTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGc                                                                                              >  7:177272/1‑149 (MQ=255)
                                                 gATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCg                                                                                            <  7:303981/148‑1 (MQ=255)
                                                 gATGTGCGGCGTCTGTGCCACGACGCCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCg                                                                                            <  8:177272/148‑1 (MQ=255)
                                                  aTGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCggg                                                                                          <  2:33858/149‑1 (MQ=255)
                                                     tgCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCt                                                                                        <  6:108301/148‑1 (MQ=255)
                                                      gCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGc                                                                                         >  3:162544/1‑146 (MQ=255)
                                                      ccgtcgtCTGTGCCACCACGCCTCCAAACCCTGCCCCCAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCAATCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGc                                                                                         <  4:162544/145‑1 (MQ=255)
                                                               gtgCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTg                                                                              >  6:210584/1‑148 (MQ=255)
                                                                tgCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                          >  2:267594/1‑87 (MQ=255)
                                                                tgCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGt                                                                                                                                          <  1:267594/87‑1 (MQ=255)
                                                                tgCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGaa                                                                            <  2:202767/149‑1 (MQ=255)
                                                                        cgTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCAcc                                                                    <  2:243466/149‑1 (MQ=255)
                                                                          tCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCgg                                                                  <  4:327656/149‑1 (MQ=255)
                                                                            gACAAAGCCTGGCGCGAGGGGCGGGTCCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgc                                                                >  5:8007/1‑149 (MQ=255)
                                                                                       gcgcGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGaa                                                      <  6:8007/148‑1 (MQ=255)
                                                                                           gAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCGAGAATTCTa                                                 <  5:210584/149‑1 (MQ=255)
                                                                                                      gCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgc                                                                >  4:35981/1‑123 (MQ=255)
                                                                                                      gCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGgc                                                                <  3:35981/123‑1 (MQ=255)
                                                                                                                gCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCAt                            >  4:157679/1‑149 (MQ=255)
                                                                                                                            ttCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCCCCCGCATGCGCTGCATGgc                >  8:138714/1‑149 (MQ=255)
                                                                                                                            ttCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATgg                 >  4:145927/1‑148 (MQ=255)
                                                                                                                              cATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATgg                 <  3:145927/146‑1 (MQ=255)
                                                                                                                                ttCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCAGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCaa            <  5:66511/149‑1 (MQ=255)
                                                                                                                                   gCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGcc                                                                                             <  6:193634/65‑1 (MQ=255)
                                                                                                                                   gCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGcc                                                                                             >  5:193634/1‑65 (MQ=255)
                                                                                                                                           aTCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCGGGc  <  8:64359/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GTACCTGTTTCATGCGCGCATCAGTGCGGCGCTCAACATCGGCCTTCTGGATGTGCGTCGTCTGTGCGACGACGTCGACAAAGCCTGGCGCGAGGGGCGGGTGCCGCTCAATGCAGCCGAAGGCTTCATTCGCCAGCTGATCGGCTGGCGTGAGTACGTGCGTGGCATCTATTGGCAGCGCATGCCGGCATATGCCGGGCTCAATCACCTGAACAATCACCGGGCGCTGCCAGAATTCTACTGGACCGGGCGCACCCGCATGCGCTGCATGGCGCAAGCCATCGGGC  >  NC_002947/3116033‑3116319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑