Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,117,504 A→G 6.0% F360L (TTT→CTT)  cfa ← cyclopropane‑fatty‑acyl‑phospholipid synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,117,5040AG 6.0% 193.3 / 2.7 67F360L (TTT→CTT) cfacyclopropane‑fatty‑acyl‑phospholipid synthase
Reads supporting (aligned to +/- strand):  ref base A (30/33);  new base G (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

GCCGATCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAGTGTGC  >  NC_002947/3117371‑3117649
                                                                                                                                     |                                                                                                                                                 
gCCGATCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCt                                                                                                                                     <  6:190668/148‑1 (MQ=255)
 ccGATCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTgg                                                                                                                                   <  7:84284/149‑1 (MQ=255)
 ccGATCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTg                                                                                                                                    >  6:291528/1‑148 (MQ=255)
  cGATCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGa                                                                                                                                  <  4:81687/149‑1 (MQ=255)
   gATCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGaa                                                                                                                                 <  5:291528/149‑1 (MQ=255)
    aTCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAAc                                                                                                                                <  3:322436/149‑1 (MQ=255)
       agccagcGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACAt                                                                                                                              <  1:81527/148‑1 (MQ=255)
         ccagcGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATgtc                                                                                                                           >  1:131374/1‑149 (MQ=255)
         ccagcGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATgtc                                                                                                                           >  4:227890/1‑149 (MQ=255)
          cagcGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATgtcg                                                                                                                          <  7:92172/149‑1 (MQ=255)
           agcGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATgtcgt                                                                                                                         <  7:337393/149‑1 (MQ=255)
               ggtGGGCTTCCGGCCCCGCCTGGGATGGGCGGCCGGCGTGCCTGGGGGGCTGCCCAGTTCATGTGCGCAACATCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGTAGAGGTAAAACTCCCACAGGCGCTGGAATATGTCGTCGTa                                                                                                                     <  8:53834/147‑1 (MQ=255)
               gTTGGACATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTa                                                                                                                     <  2:171554/149‑1 (MQ=255)
                ttGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTcgtcgt                                                                                                                      <  8:143783/147‑1 (MQ=255)
                ttGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTcgtcgt                                                                                                                      >  7:143783/1‑147 (MQ=255)
                      aTCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTaaaa                                                                                                                                                   >  4:10459/1‑112 (MQ=255)
                      aTCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTaaaa                                                                                                                                                   <  3:10459/112‑1 (MQ=255)
                       tCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGt                                                                                                             <  7:184937/149‑1 (MQ=255)
                               cGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAgc                                                                                                     >  1:13785/1‑149 (MQ=255)
                                gCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGGCGTAGCCAAGGTCCGTCAgcg                                                                                                    >  2:276225/1‑149 (MQ=255)
                                              cACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGa                                                                                      <  3:227890/149‑1 (MQ=255)
                                               aCGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGAc                                                                                     >  5:161487/1‑149 (MQ=255)
                                               aCGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGAc                                                                                     >  6:266734/1‑149 (MQ=255)
                                                  gCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCa                                                                                  <  5:266734/149‑1 (MQ=255)
                                                   cGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAg                                                                                 <  1:276225/149‑1 (MQ=255)
                                                       ccTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTcgtcgt                                                                                                                      <  1:114614/108‑1 (MQ=255)
                                                       ccTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTcgtcgt                                                                                                                      >  2:114614/1‑108 (MQ=255)
                                                          ggggTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAGCTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCa                                                                          >  4:86413/1‑149 (MQ=255)
                                                          ggggTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAGCTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCa                                                                          >  8:42680/1‑149 (MQ=255)
                                                                  gcCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCa                                                                                                  >  8:209982/1‑117 (MQ=255)
                                                                   cccAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAgtg                                                                  <  2:131374/148‑1 (MQ=255)
                                                                    ccAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTc                                                                <  2:13785/149‑1 (MQ=255)
                                                                    ccAGAGCAGGTGCGCAACACCGATCGCGCGATCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAgg                                                                                <  2:300742/133‑1 (MQ=255)
                                                                    ccAGAGCAGGTGCGCAACACCGATCGCGCGATCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAgg                                                                                >  1:300742/1‑133 (MQ=255)
                                                                                    acacCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAGCTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTa                                                <  7:42680/149‑1 (MQ=255)
                                                                                    acacCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAGCTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTa                                                <  3:86413/149‑1 (MQ=255)
                                                                                      acCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGCCGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGt                                              >  8:240603/1‑149 (MQ=255)
                                                                                      acCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCACTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGt                                              >  5:318006/1‑149 (MQ=255)
                                                                                           tCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAgg                                         <  7:191073/149‑1 (MQ=255)
                                                                                                    cctcGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCt                                >  1:143173/1‑149 (MQ=255)
                                                                                                    cctcGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCt                                >  1:319753/1‑149 (MQ=255)
                                                                                                     ctcGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTc                                                                           >  4:138162/1‑105 (MQ=255)
                                                                                                     ctcGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTc                                                                           <  3:138162/105‑1 (MQ=255)
                                                                                                     ctcGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCtt                               >  6:143037/1‑149 (MQ=255)
                                                                                                        gAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCa                            >  1:37008/1‑149 (MQ=255)
                                                                                                         aaaCCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCAt                           >  5:237117/1‑149 (MQ=255)
                                                                                                         aaaCCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCAt                           >  6:167292/1‑149 (MQ=255)
                                                                                                          aaCCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCAtg                          >  8:101609/1‑149 (MQ=255)
                                                                                                            ccGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCAtgtg                        <  2:59930/149‑1 (MQ=255)
                                                                                                            ccGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCAtgtg                        >  8:283750/1‑149 (MQ=255)
                                                                                                              gCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGca                      <  6:237117/149‑1 (MQ=255)
                                                                                                                  tGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGtgtg                                                      <  5:50394/113‑1 (MQ=255)
                                                                                                                  tGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGtgtg                                                      >  6:50394/1‑113 (MQ=255)
                                                                                                                    gCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAg                  <  6:161487/147‑1 (MQ=255)
                                                                                                                    gCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGt                >  1:333593/1‑149 (MQ=255)
                                                                                                                       gTAGCAGAGGTAAAACTCCCACAGGCACTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCa             <  6:318006/149‑1 (MQ=255)
                                                                                                                        tAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAg            <  5:167292/149‑1 (MQ=255)
                                                                                                                         aGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAg            <  2:319753/148‑1 (MQ=255)
                                                                                                                          gCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGc          <  2:37008/149‑1 (MQ=255)
                                                                                                                           cAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTgg                                                     >  7:297762/1‑105 (MQ=255)
                                                                                                                           cAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTgg                                                     <  8:297762/105‑1 (MQ=255)
                                                                                                                            agagGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCa        <  5:143037/149‑1 (MQ=255)
                                                                                                                              agGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAgt      >  7:130941/1‑149 (MQ=255)
                                                                                                                              agGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAg       <  8:130941/148‑1 (MQ=255)
                                                                                                                                gTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAgtgt    >  5:14332/1‑149 (MQ=255)
                                                                                                                                gTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAgtgt    >  4:203693/1‑149 (MQ=255)
                                                                                                                                 tAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCg                                                               <  3:176869/89‑1 (MQ=255)
                                                                                                                                 tAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCg                                                               >  4:176869/1‑89 (MQ=255)
                                                                                                                                 tAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAGtgtg   >  4:282288/1‑149 (MQ=255)
                                                                                                                                   aaaCTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAGTGTGc  <  3:282288/148‑1 (MQ=255)
                                                                                                                                   aaaCTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAGTGTGc  <  1:87747/148‑1 (MQ=255)
                                                                                                                                   aaaCTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAGTGTGc  >  2:87747/1‑148 (MQ=255)
                                                                                                                                     |                                                                                                                                                 
GCCGATCAGCCAGCGGTTGGGCATCAGGCACCGCCAGGCAAGGGCGCACGGCGTGCCTGGGGTGCTGCCCAGAGCAGGTGCGCAACACCGATCGCGCGCTCCTCGAAACCGCCCTGGCAGTAGCAGAGGTAAAACTCCCACAGGCGCTGGAACATGTCGTCGTAGCCAAGGTCCGTCAGCGCAGTGCGTGCCTGACGCAGGTTTTCACGCCAGTGTCGCAGGGTGTGGGCGTAGTCCAGGCCAAAATCTTCCATGTGCACAAGGTTCAGGCCAGTGTGC  >  NC_002947/3117371‑3117649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑