Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,129,459 T→C 7.5% F236L (TTC→CTC)  dmoA‑I → dimethyl‑sulfide monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,129,4590TC 7.5% 148.7 / 2.7 53F236L (TTC→CTC) dmoA‑Idimethyl‑sulfide monooxygenase
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base C (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCAACCTGCAGACGGTGATCGT  >  NC_002947/3129313‑3129607
                                                                                                                                                  |                                                                                                                                                     
gcgGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTc                                                                                                                                                     <  5:191959/149‑1 (MQ=255)
gcgGCATTAGCAGCGATGCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTc                                                                                                                                                     <  6:302867/149‑1 (MQ=255)
 cgGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCATCGAGCCGTGGCAAGGACTTCg                                                                                                                                                    >  2:263634/1‑149 (MQ=255)
    cATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACCTCGCTg                                                                                                                                                 >  4:16249/1‑149 (MQ=255)
    cATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACCTCGCTg                                                                                                                                                 >  4:11412/1‑149 (MQ=255)
     aTTTTCAGCGATCCGAACAAGGTTCATGAGATCCGACACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGc                                                                                                                                                >  1:53675/1‑149 (MQ=255)
           aGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACCTCGCTGCCGGGc                                                                                                                                           <  3:11412/148‑1 (MQ=255)
           aGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACCTCGCTGCCGGGc                                                                                                                                           <  3:16249/148‑1 (MQ=255)
            gCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCAc                                                                                                                                         >  2:60340/1‑149 (MQ=255)
                  cGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGa                                                                                                                                    <  8:134779/148‑1 (MQ=255)
                               tGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTtcgtcg                                                                                                                      >  7:286421/1‑149 (MQ=255)
                                gagaTCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCg                                                                                                                               >  3:284920/1‑139 (MQ=255)
                                gagaTCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCg                                                                                                                               <  4:284920/139‑1 (MQ=255)
                                           ccaGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTcc                                                                                                           >  7:36767/1‑148 (MQ=255)
                                           ccaGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTcc                                                                                                           >  4:201710/1‑148 (MQ=255)
                                              gggCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAgg                                                                                                       <  8:229014/149‑1 (MQ=255)
                                                  aaGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGAt                                                                                                   >  4:138423/1‑149 (MQ=255)
                                                  aaGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGAt                                                                                                   >  4:143767/1‑149 (MQ=255)
                                                   aGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATc                                                                                                  <  1:60340/149‑1 (MQ=255)
                                                       cTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTga                                                                                              <  4:240444/149‑1 (MQ=255)
                                                        tttCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTg                                                                                               >  4:30192/1‑147 (MQ=255)
                                                          tCAGGGGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTgaa                                                                                             <  4:75873/147‑1 (MQ=255)
                                                             ggTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑aaaa                                                                                       >  2:21520/1‑149 (MQ=255)
                                                                    ggCAGCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGc                                                                                <  4:231844/149‑1 (MQ=255)
                                                                      catccatcTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCTAAGGTGATCCTGAAG‑AAAACCGTGGCCg                                                                              <  1:21520/149‑1 (MQ=255)
                                                                          catcTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACAt                                                                          >  3:99172/1‑149 (MQ=255)
                                                                                  cGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTc                                                                  <  8:286421/149‑1 (MQ=255)
                                                                                      ccTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCgcgc                                                              >  8:20875/1‑149 (MQ=255)
                                                                                        ttCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTg                                                            >  8:67441/1‑149 (MQ=255)
                                                                                        ttCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTg                                                            >  5:34482/1‑149 (MQ=255)
                                                                                        ttCGCCACAGCGCACGCCGGTCCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTg                                                            >  7:39886/1‑149 (MQ=255)
                                                                                           gCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCg                                                         <  7:20875/149‑1 (MQ=255)
                                                                                               caGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGc                                                     >  6:114335/1‑149 (MQ=255)
                                                                                                      cGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGt                                              <  3:143767/149‑1 (MQ=255)
                                                                                                      cGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGt                                              <  3:138423/149‑1 (MQ=255)
                                                                                                         cGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGAt                                           >  6:208774/1‑149 (MQ=255)
                                                                                                          ggTGCTCTATCAGGCGGGGGCATCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAGCAAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGAt                                           <  1:263634/149‑1 (MQ=255)
                                                                                                                 tATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGc                                     <  5:114335/147‑1 (MQ=255)
                                                                                                                   tctggCGGGGGCGTCGAGCCGTTGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTTATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAg                                  <  7:203387/145‑1 (MQ=255)
                                                                                                                   tCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGt                                              >  3:274025/1‑136 (MQ=255)
                                                                                                                   tCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGt                                              <  4:274025/136‑1 (MQ=255)
                                                                                                                      ggCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGc                              >  5:68017/1‑149 (MQ=255)
                                                                                                                      ggCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGc                              >  1:309663/1‑149 (MQ=255)
                                                                                                                       gCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACCCAAg                                  >  8:203387/1‑144 (MQ=255)
                                                                                                                              cGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTc                      <  2:309663/149‑1 (MQ=255)
                                                                                                                              cGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTc                      <  3:201710/149‑1 (MQ=255)
                                                                                                                                tCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCaa                    <  6:68017/149‑1 (MQ=255)
                                                                                                                                     ccGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCAACCTg                >  2:73856/1‑148 (MQ=255)
                                                                                                                                     ccGGGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCAACCTGc               <  2:53675/149‑1 (MQ=255)
                                                                                                                                       gTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAg                                  <  6:321243/128‑1 (MQ=255)
                                                                                                                                       gTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAg                                  >  5:321243/1‑128 (MQ=255)
                                                                                                                                            aaGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCAACCTGCAGACgg         <  2:15435/148‑1 (MQ=255)
                                                                                                                                            aaGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCAACCTGCAGACgg         >  1:15435/1‑148 (MQ=255)
                                                                                                                                                  ttCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCAACCTGCAGACGGTGAtcgt  >  5:147860/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCGGCATTTTCAGCGATCCGAACAAGGTTCATGAGATCCGCCACCAGGGCAAGCACTTTCAGGTGCCCGGCATCCATCTTTGCGAGCCTTCGCCACAGCGCACGCCGGTGCTCTATCAGGCGGGGGCGTCGAGCCGTGGCAAGGACTTCGCTGCCGGGCACGCCGAGTGCGTGTTCGTCGCCGCACCGTCCAAGGTGATCCTGAAG‑AAAACCGTGGCCGACATCCGCCGTCGCGCTGCCGAGGCCGGGCGTGATCCACGCAAGGTGCTGATCTTCAACCTGCAGACGGTGATCGT  >  NC_002947/3129313‑3129607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑