Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,140,401 G→A 7.5% A319T (GCT→ACT)  sfnB → SfnB family sulfur acquisition oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,140,4010GA 7.5% 153.0 / 2.3 53A319T (GCT→ACT) sfnBSfnB family sulfur acquisition oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base A (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

CTTTGCCGAAACCCTGCGTGTGGCGCGTCAGGCACGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTGGGCGGCACG  >  NC_002947/3140256‑3140544
                                                                                                                                                 |                                                                                                                                                
cTTTGCCGAAACCCTGCGTGTGGCGCGTCAGGCACGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTg                                                                                                                                               <  7:200526/149‑1 (MQ=255)
     ccGAAACCCTGCGTGTGGCGCGTCAGGCACGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGACCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAg                                                                                                                                          <  7:239916/149‑1 (MQ=255)
           cccTGCGTGTGGCGCGTCAGGCACGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGtt                                                                                                                                    <  5:203557/149‑1 (MQ=255)
           cccTGCGTGTGGCGCGTCAGGCACGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGtt                                                                                                                                    <  2:250961/149‑1 (MQ=255)
                         cgTCAGGCACGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTAGc                                                                                                                      >  7:300912/1‑149 (MQ=255)
                                cACGCCGCTGGGTCGTCTGCGGCTTGGAGCATGGGTGGCAGGATCGAGTTGGCCAGGCACTGATGGGCGAACTGGCCTGGCGCCTGGAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCt                                                                                                               <  8:300912/149‑1 (MQ=255)
                                 aCGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGc                                                                                                                                        <  7:325884/123‑1 (MQ=255)
                                 aCGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGc                                                                                                                                        >  8:325884/1‑123 (MQ=255)
                                 aCGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGcc                                                                                                                >  1:55303/1‑147 (MQ=255)
                                   gCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGc                                                                                                            >  6:348419/1‑149 (MQ=255)
                                       cTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCgcgc                                                                                                                            <  8:120749/129‑1 (MQ=255)
                                       cTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCgcgc                                                                                                                            >  7:120749/1‑129 (MQ=255)
                                            tCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGt                                                                                                                                     >  6:121363/1‑115 (MQ=255)
                                            tCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGt                                                                                                                                     <  5:121363/115‑1 (MQ=255)
                                             cGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACg                                                                                                  <  3:201644/149‑1 (MQ=255)
                                               aCAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGtt                                                                                                >  6:29211/1‑149 (MQ=255)
                                                      cTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAg                                                                                         <  5:348419/149‑1 (MQ=255)
                                                         gcagcaTGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCt                                                                                      >  8:175991/1‑149 (MQ=255)
                                                                   tggcAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTggtggt                                                                            >  7:49471/1‑149 (MQ=255)
                                                                       aGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCATTGGTGGTCggc                                                                        >  5:21928/1‑149 (MQ=255)
                                                                       aGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCggt                                                                        <  2:55303/149‑2 (MQ=255)
                                                                           tCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCggccagg                                                                    >  7:43590/1‑149 (MQ=255)
                                                                              aCTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAg                                                                                   <  2:275708/131‑1 (MQ=255)
                                                                              aCTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAg                                                                                   >  1:275708/1‑131 (MQ=255)
                                                                               cTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCggccaggcc                                                                  >  8:329617/1‑147 (MQ=255)
                                                                                   gCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCACTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAgtgg                                                                                >  5:201880/1‑129 (MQ=255)
                                                                                   gCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCACTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAgtgg                                                                                <  6:201880/129‑1 (MQ=255)
                                                                                          aCTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCTGCCAGGCCAAGGTGCT‑GTCTa                                                    >  7:288625/1‑149 (MQ=255)
                                                                                           cTGATCGGCGAACTGGCCGGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTAc                                                   <  6:327084/149‑1 (MQ=255)
                                                                                            tGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTAcc                                                  >  2:152051/1‑149 (MQ=255)
                                                                                              aTCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCga                                                <  5:29211/149‑1 (MQ=255)
                                                                                                  gCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCACTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGAtt                                            >  6:277572/1‑149 (MQ=255)
                                                                                                       ctggcctggcGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTccc                                        >  2:223737/1‑148 (MQ=255)
                                                                                                          gcctggcGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTcc                                         <  4:34537/144‑1 (MQ=255)
                                                                                                          gcctggcGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTcc                                         >  3:34537/1‑144 (MQ=255)
                                                                                                            ctggcGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAgg                                  >  7:209474/1‑149 (MQ=255)
                                                                                                            ctggcGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCACGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAg                                                               >  4:95359/1‑121 (MQ=255)
                                                                                                            ctggcGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCACGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAg                                                               <  3:95359/121‑1 (MQ=255)
                                                                                                                   cTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTagag                                   >  2:133285/1‑141 (MQ=255)
                                                                                                                   cTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTagag                                   <  1:133285/141‑1 (MQ=255)
                                                                                                                      cAGGCGGCCGAGGGGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCTCGTCTACCGAGATTTCCCTAGAGGCGTCCAGTc                         <  1:223737/149‑1 (MQ=255)
                                                                                                                      cAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTc                         <  1:152051/148‑1 (MQ=255)
                                                                                                                         gcggcCGAGGCGATCTTGCAGCGCACTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCct                     <  5:277572/149‑1 (MQ=255)
                                                                                                                               gAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCctgctg                 >  1:240718/1‑147 (MQ=255)
                                                                                                                                aGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAg              <  8:209474/149‑1 (MQ=255)
                                                                                                                                 ggCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGc             <  7:175991/149‑1 (MQ=255)
                                                                                                                                    gATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTgg          >  6:232058/1‑149 (MQ=255)
                                                                                                                                    gATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTgg          >  8:131904/1‑149 (MQ=255)
                                                                                                                                          gCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTggg         <  8:49471/144‑1 (MQ=255)
                                                                                                                                          gCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTgg          <  7:131904/143‑1 (MQ=255)
                                                                                                                                          gCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTGGGCGGCa    >  2:67609/1‑149 (MQ=255)
                                                                                                                                           cAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTGGGCGGCAc   <  2:240718/149‑1 (MQ=255)
                                                                                                                                            aGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTGGGCGGCAcg  >  7:73492/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTTTGCCGAAACCCTGCGTGTGGCGCGTCAGGCACGCCCCTGGGTCGACAGCGGCTTGCAGCATGGCTGGCAGGATCCACTTGGCCAGGCACTGATCGGCGAACTGGCCTGGCGCCTGCAGGCGGCCGAGGCGATCTTGCAGCGCGCTGCCGAGGCCGTTGATCGCGCCGTCGCCGAGCCTAGCGAAGAGCACGTTGCTGAAGCCTCAGTGGTGGTCGGCCAGGCCAAGGTGCT‑GTCTACCGAGATTTCCCTAGAGGCGTCCAGTCGCCTGCTGGAGCTGGGCGGCACG  >  NC_002947/3140256‑3140544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑