Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 211,621 G→C 6.2% V5709V (GTG→GTC)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947211,6210GC 6.2% 207.5 / 3.8 64V5709V (GTG→GTC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (26/34);  new base C (2/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.99e-01

TGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCA  >  NC_002947/211477‑211766
                                                                                                                                                |                                                                                                                                                 
tGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGAcc                                                                                                                                                >  2:44194/1‑148 (MQ=255)
   ccAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAg                                                                                                                                            >  8:152791/1‑149 (MQ=255)
    cAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAgg                                                                                                                                           >  5:28746/1‑149 (MQ=255)
      gATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAtgtg                                                                                                                                         <  5:252431/149‑1 (MQ=255)
      gATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtg                                                                                                                                         <  1:183723/149‑1 (MQ=255)
       aTCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtgg                                                                                                                                        >  6:115250/1‑149 (MQ=255)
       aTCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTCACCGAAggcgt                                                                                                                                        >  8:91627/1‑146 (MQ=255)
        tCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtggt                                                                                                                                       <  7:158812/149‑1 (MQ=255)
          aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGgtggtg                                                                                                                                      <  1:9631/148‑1 (MQ=255)
          aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGgtggtg                                                                                                                                      <  3:109374/148‑1 (MQ=255)
          aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTga                                                                                                                                     <  5:274518/149‑1 (MQ=255)
          aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTga                                                                                                                                     <  3:321257/149‑1 (MQ=255)
          aGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTCACCGAAggcgtcgc                                                                                                                                     <  7:91627/149‑7 (MQ=255)
           gCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAggtggt                                                                                                                                       >  4:128364/1‑146 (MQ=255)
           gCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACACCTTCGGTCACCGAAggcgtcgcg                                                                                                                                    <  3:274869/149‑8 (MQ=255)
                ggCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATc                                                                                                                                 <  6:28746/147‑1 (MQ=255)
                ggCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATc                                                                                                                                 <  6:349893/147‑1 (MQ=255)
                    tCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTac                                                                                                                           <  7:151245/149‑1 (MQ=255)
                      aaCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTacac                                                                                                                         <  5:11392/149‑1 (MQ=255)
                      aaCTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTacac                                                                                                                         <  3:253979/149‑1 (MQ=255)
                           ggcggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATc                                                                                                                     <  1:44194/148‑1 (MQ=255)
                             cggTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCAcc                                                                                                                  <  8:319919/149‑1 (MQ=255)
                               gTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCt                                                                                                                >  5:88864/1‑149 (MQ=255)
                                tGGACGCCACTGGTGCGACCTTCGAGACCCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGAGCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCt                                                                                                                >  1:357778/1‑148 (MQ=255)
                                  gACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGAc                                                                                                             >  2:230988/1‑149 (MQ=255)
                                     gCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTga                                                                                                                                     >  7:154688/1‑122 (MQ=255)
                                     gCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTga                                                                                                                                     <  8:154688/122‑1 (MQ=255)
                                       cACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACcaac                                                                                                         >  4:24976/1‑148 (MQ=255)
                                        aCTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACcaacaa                                                                                                       <  7:74965/149‑1 (MQ=255)
                                         cTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACaaa                                                                                                      <  6:88864/149‑1 (MQ=255)
                                            gTGCGACGTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGAt                                                                                                   <  2:258136/149‑1 (MQ=255)
                                               cGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGt                                                                                                <  5:147216/149‑1 (MQ=255)
                                                   cTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCt                                                                                              <  4:105341/147‑1 (MQ=255)
                                                   cTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCt                                                                                              >  3:105341/1‑147 (MQ=255)
                                                   cTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGc                                                                                            <  7:152791/149‑1 (MQ=255)
                                                    ttCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGa                                                                                                                                                  <  2:322662/94‑1 (MQ=255)
                                                    ttCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGa                                                                                                                                                  >  1:322662/1‑94 (MQ=255)
                                                      cGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGa                                                                                         >  2:230257/1‑149 (MQ=255)
                                                                gAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCaacaac                                                                                 <  6:146450/147‑1 (MQ=255)
                                                                gAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCaacaac                                                                                 <  5:115250/147‑1 (MQ=255)
                                                                  gCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACcaca                                                                             <  3:24976/149‑1 (MQ=255)
                                                                     gggCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACca                                                                                                           <  5:75340/116‑1 (MQ=255)
                                                                     gggCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACca                                                                                                           >  6:75340/1‑116 (MQ=255)
                                                                               gCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGCTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCt                                                                 >  7:232309/1‑148 (MQ=255)
                                                                               gCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGCTGACCGAAGGTGGTGACATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCtt                                                                >  7:228326/1‑149 (MQ=255)
                                                                                   cGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACcaacaa                                                                                                       <  1:114160/106‑1 (MQ=255)
                                                                                   cGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACcaacaa                                                                                                       >  2:114160/1‑106 (MQ=255)
                                                                                      ttCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCt                                                          >  2:277387/1‑148 (MQ=255)
                                                                                                cGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGAc                                                                   <  1:317596/129‑1 (MQ=255)
                                                                                                      caccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCg                                         >  6:230039/1‑149 (MQ=255)
                                                                                                        ccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGtc                                       <  1:230257/149‑1 (MQ=255)
                                                                                                        ccGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGt                                        >  2:7933/1‑148 (MQ=255)
                                                                                                           acgaAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGtcat                                     >  6:189808/1‑148 (MQ=255)
                                                                                                                 tggtggCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTg                              >  3:101775/1‑149 (MQ=255)
                                                                                                                        cAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATc                                                                                       >  3:262319/1‑85 (MQ=255)
                                                                                                                        cAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATc                                                                                       <  4:262319/85‑1 (MQ=255)
                                                                                                                          aGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAAt                     <  5:189808/149‑1 (MQ=255)
                                                                                                                          aGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAAt                     <  1:7933/149‑1 (MQ=255)
                                                                                                                           gCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATg                    >  5:202849/1‑149 (MQ=255)
                                                                                                                                    gACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACATCGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTgg           <  2:357778/149‑1 (MQ=255)
                                                                                                                                    accaccTTCGGTCACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTgg           >  2:305453/5‑149 (MQ=255)
                                                                                                                                            cGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCgg                          <  3:245244/126‑1 (MQ=255)
                                                                                                                                            cGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCgg                          >  4:245244/1‑126 (MQ=255)
                                                                                                                                             ggTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCa  >  2:122309/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGGCCAGATCAGCCTGGGCATCAACTCGGCGGTGGACGCCACTGGTGCGACCTTCGAGAACCTCGAGCTGGGCGGTGCGGCTTCGGTTCAAGTGACCGATACCACCGACGAAGTGGTGGCCAAGCTGACCGCGACGCCTTCGGTGACCGAAGGTGGTGAGATCACCTACACCATCACCCTGACCAACAAAGATGGTCTGCCGATCAACAACCACAGTGCATTGACCTTCACCCTGAGCGACGGCAAAACCGTCATCACCGTGCCGGCTAATGGCACCGTGGGTACTGCCA  >  NC_002947/211477‑211766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑